G-CSF (granulocyte-colony stimulating factor) is a secreted glycoprotein that regulates the proliferation, differentiation, and activation of neutrophilic granulocyte lineage cells. It also enhances M-CSF induced monocyte differentiation and the proliferation of Th2-inducing dendritic cells. G-CSF promotes the development of T cell immune tolerance as well as tissue recovery following myocardial infarction and cerebral ischemia. It signals through G-CSF R/CD114 on monocytes, neutrophils, megakaryocytes, platelets, myeloid progenitors, trophoblasts and placenta, endothelial cells, and various tumor cell types. Mutations in G-CSF R are associated with congenital neutropenia.
Human G-CSF Magnetic Luminex® Performance Assay
R&D Systems | Catalog # LUHM214
Luminex High Performance Assays
Human G-CSF Magnetic Luminex® Performance Assay is specifically designed for multiplex detection of human G-CSF with a panel of related analytes in serum, plasma, or tissue culture supernatants. Luminex High Performance Assays go through rigorous development, validation, and quality control testing to ensure the best performance of your multiplex assay.
This assay is customizable, select some or all the analytes in the entire panel. Customize your panel with the Luminex Assay Customization Tool.
Key Product Details
Panel
Human Cytokine Panel A
Assay Type
Magnetic bead-based multiplex assay for the Luminex® platform
Format
1 x 96-well microplate and magnetic antibody-coated beads
Species
Human
Analytes Detected
G-CSF
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Product Features
Magnetic Luminex High Performance Assays are flexible bead-based multiplex assays. They allow up to 50 user-defined target analytes to be simultaneously profiled using cell culture supernates, serum, or plasma samples. Some panels are also validated with human milk, saliva, and/or urine samples.
- Simultaneously profile Human G-CSF with a panel of related analytes in one sample
- Magnetic format allows for easier wash steps
- Undergoes similar validation testing as Quantikine® ELISA Kits
- Precise and Specific
- Requires a small sample volume (<50 µL)
- Run your assay in just 3-5 hours
- Mass-calibrated standards for consistent results with every new lot of material
- Enough reagents for one 96-well plate
- Quick turn-around time: Kits are typically shipped within 2-3 days of ordering*
*Please allow additional time for international orders.
Performance Validation
R&D Systems Luminex High Performance Assays undergo our most extensive validation testing. All Luminex High Performance Assay Panels are validated for use with serum and plasma samples. Some High Performance Assays are additionally validated for cell culture supernatants, milk, saliva, or urine, as indicated on the individual product data sheets. Luminex High Performance Assays are tested for sensitivity (three-quarters the low standard), intra-assay precision, inter-assay precision, and to ensure assay linearity for validated sample types. Recovery values for individual samples in validated sample types are also tested. Validation data for each analyte can be found in the product datasheet.
Base Kit Contents
- Standard Cocktail(s)
- Bead Diluent
- Biotin Antibody Diluent
- Calibrator Diluent
- Wash Buffer
- Streptavidin-PE
- One flat-bottom 96-well Microplate
- Foil Plate Sealers (4)
- Mixing Bottle
- Standard Value Card**
**A standard curve must be generated each time an assay is run, utilizing values from the Certificate of Analysis provided with each Luminex Assay.
Bead Set Contents
- User-mixed antibody-coated Magnetic beads in individual vials
- User-mixed cocktail of biotinylated detection antibody
Precision
|
Intra-assay Precision (precision within an assay) - Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Inter-assay Precision (precision between assays) - Three samples of known concentration were tested in twenty-five separate assays to assess precision between assays. |
||||||
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 25 | 25 | 25 |
| Mean (pg/mL) | 17.1 | 31.7 | 508 | 20.1 | 240 | 440 |
| Standard deviation | 1.2 | 2.1 | 10.9 | 1.9 | 33 | 22 |
| CV (%) | 7.1 | 5.3 | 2.1 | 9.5 | 13.9 | 4.9 |
Recovery
|
Samples containing and/or spiked with human G-CSF were evaluated for recovery. |
||||||
| Sample Type | Average % Recovery | Range (%) | ||||
|---|---|---|---|---|---|---|
| Cell culture supernate | 95 | 75-110 | ||||
| Serum | 102 | 88-113 | ||||
| EDTA plasma | 91 | 92-117 | ||||
| Heparin plasma | 88 | 77-101 | ||||
Linearity
|
Samples containing and/or spiked with human G-CSF were serially diluted to evaluate assay linearity. |
|||||||||
| Cell culture supernate | Serum | EDTA plasma | Heparin plasma | ||||||
|---|---|---|---|---|---|---|---|---|---|
| 1:2 | Average % of Expected | 103 | 109 | 106 | 113 | ||||
| Range (%) | 90-113 | 100-115 | 82-118 | 105-122 | |||||
| 1:4 | Average % of Expected | 110 | 107 | 107 | 114 | ||||
| Range (%) | 84-131 | 85-120 | 70-122 | 108-119 | |||||
| 1:8 | Average % of Expected | 114 | 113 | 117 | 102 | ||||
| Range (%) | 80-138 | 85-129 | 102-129 | 111-123 | |||||
Which Luminex Assay is Right for Me?
Assays for the Luminex platform are offered as High Performance Assays or Discovery Assays.
High Performance Assays are optimized and validated as complete panels with a focused set of analytes. You can choose from Fixed Panels, Premixed options, or Configurable User Mix formats depending on your needs.
Discovery Assays deliver maximum flexibility, enabling the creation of fully customized panels tailored to your research needs. With universal reagents validated for compatibility across all analytes in the menu, they support endless customization possibilities.
Additional Information About Luminex Assays
All R&D Systems Luminex Assays are designed for use with any Luminex instrument including MAGPIX, Luminex 200, FLEXMAP 3D, and the INTELLIFLEX.
Luminex assays use color-coded, magnetic microparticles coated with analyte-specific antibodies to enable multiplex quantitation. During the assay, standards and samples are added to the wells, where target analytes bind to the capture antibodies. A biotinylated detection antibody cocktail is then introduced, followed by a Streptavidin-PE conjugate that generates a fluorescent signal proportional to the amount of analyte present.
Each microparticle is identified by a unique signature and the signal intensity is measured using a Luminex instrument. These assays are designed to deliver reliable and reproducible results with validated antibody pairs and optimized protocols. They are well suited for applications that require sensitivity, accuracy, and confidence in your data while enabling efficient multiplexing to measure multiple targets simultaneously.
Scientific Data Images for Human G-CSF Magnetic Luminex® Performance Assay
Quantist software graph of signal intensity versus concentration for G-CSF in the Human Cytokine A Luminex Panel
Preparation and Storage
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: G-CSF
Long Name
Granulocyte Colony Stimulating Factor
Alternate Names
C17orf33, CSF3, CSF3OS, Filgrastim, GCSF, Lenograstim, Pluripoietin
Gene Symbol
CSF3
Additional G-CSF Products
Product Documents for Human G-CSF Magnetic Luminex® Performance Assay
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human G-CSF Magnetic Luminex® Performance Assay
Luminex® is a registered trademark of Luminex Corporation.
For research use only
Citations for Human G-CSF Magnetic Luminex® Performance Assay
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Sample Tested: Saliva and EDTA PlasmaVerified Customer | Posted 08/23/2017The assay performed as expected with low % CV's
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