Human GAK Antibody

Catalog #: MAB6918
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB6918
MAB6918-SP

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Product Details

Citations (1)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human GAK Antibody Summary

Species Reactivity

Human

Specificity

Detects human GAK in direct ELISAs and Western blots.

Source

Monoclonal Mouse IgG_2B Clone # 706306

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human GAK
Phe258-Asp443
Accession # O14976

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human GAK by Western Blot. Western blot shows lysates of Daudi human Burkitt's lymphoma cell line and BJAB human Burkitt's lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human GAK Monoclonal Antibody (Catalog # MAB6918) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for GAK at approximately 144 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GAK

Cyclin G-associated kinase (GAK), also known as Auxilin 2, is an approximately 140 kDa widely expressed protein that associates with CDK5. GAK promotes microtubule growth during mitotic spindle assembly and is required for centrosome maturation, chromosome congression, and mitotic progression. It also regulates the assembly and intracellular trafficking of clathrin-coated vesicles. GAK contains a Ser/Thr protein kinase domain (aa 40-314), a phosphatase tensin/auxilin domain (aa 399-566), a PTEN C2 domain (aa 570-708), and a J domain (aa 1247-1311). Within aa 258-443, human GAK shares 88% aa sequence identity with mouse and rat GAK.

Long Name

Cyclin G-associated Kinase

Entrez Gene IDs

2580 (Human); 231580 (Mouse); 81659 (Rat)

Alternate Names

Auxillin 2; cyclin G associated kinase; cyclin-G-associated kinase; EC 2.7.11.1; FLJ16629; FLJ40395; GAK; MGC99654

Product Datasheets

Product Datasheet

COA

SDS

Citation for Human GAK Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

Regulation of senescence escape by the cdk4-EZH2-AP2M1 pathway in response to chemotherapy
Authors: M Le Duff, J Gouju, B Jonchère, J Guillon, B Toutain, A Boissard, C Henry, C Guette, E Lelièvre, O Coqueret
Cell Death Dis, 2018-02-07;9(2):199.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot