Human HSP90 alpha Antibody

R&D Systems | Catalog # A-440

Product is in the process of being discontinued.
R&D Systems
Discontinued Product
A-440 has been discontinued. View all HSP90 alpha products.

Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all HSP90 alpha Primary Antibodies »

Product Specifications

Immunogen

Purified, recombinant human HSP90 alpha protein
Accession # P07900

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Human HSP90 alpha Antibody

Detection of Human HSP90a antibody by Western Blot.

Detection of Human HSP90 alpha by Western Blot.

Western blot shows lysates of HEK293 human embryonic kidney cell line. PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Human HSP90a Antigen Affinity-purified Polyclonal Antibody (Catalog # A-440) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for HSP90a at approximately 85-95 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human HSP90 alpha Antibody

Application
Recommended Usage

Western Blot

0.5 µg/mL
Sample: HEK293 human embryonic kidney cell line

Formulation, Preparation, and Storage

Formulation

Supplied as a solution in PBS containing Glycerol and Sodium Azide.

Shipping

The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

Background: HSP90 alpha

The heat shock protein-90 kDa (HSP90) is a composite name for a large group of genes whose molecular weights average 90 kDa. HSP90 functions primarily as a molecular chaperone, facilitating the folding of other cellular proteins, preventing protein aggregation, or targeting improperly folded proteins to specific degradative pathways. In addition to its role as a molecular chaperone and stress response protein, HSP90 is a central component in a number of cellular processes including hormone signaling and cell cycle control. HSP90 is ubiquitously expressed, highly conserved, and accounts for 1-2% of the total cellular protein. Eukaryotic cells have two principal forms of HSP90 including the inducible HSP90 alpha (also known as HSP90AA1, HSP90A, HSPC1, HSPCA and HSP86) and the constitutively expressed HSP90 beta (also known as HSP90AB1, HSP90B, HSPCB, HSPC2, and HSP89-beta. While HSP90 alpha and HSP90 beta share 90% identity, this affinity-purified polyclonal antibody is specific for HSP90 alpha.

References

Hartl F.U. & Hayer-Hartl M. (2009) Nat. Struc. Mol. Biol. 16: 574-581 Kampinga H. H. & Craig E.A. (2010) Nat. Rev. Mol. Cell Biol. 11: 579-592

Long Name

Heat Shock Protein 90 alpha

Alternate Names

HSP86, HSP89A, HSP90A, HSP90AA1, HSP90N, HSPC1, HSPCA, HSPCAL1, HSPCAL4, HSPN, LAP2

Entrez Gene IDs

3320 (Human); 15519 (Mouse); 299331 (Rat)

Gene Symbol

HSP90AA1

UniProt

P07900

Additional HSP90 alpha Products

Product Documents for Human HSP90 alpha Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human HSP90 alpha Antibody

For research use only

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FAQs

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Associated Pathways

Apoptosis Signaling Pathway
Apoptosis Signaling Pathway Thumbnail