Human ICAM-4 APC-conjugated Antibody

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Detection of ICAM‑4 in Human Red Blood Cells by Flow Cytometry.
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Human ICAM-4 APC-conjugated Antibody Summary

Species Reactivity
Detects human ICAM-4 in direct ELISAs.
Monoclonal Mouse IgG2B Clone # 729632
Protein A or G purified from hybridoma culture supernatant
Chinese hamster ovary cell line CHO-derived recombinant human ICAM-4
Accession # Q14773
Supplied in a saline solution containing BSA and Sodium Azide.
Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)


Recommended Concentration
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of ICAM‑4 antibody in Human Red Blood Cells antibody by Flow Cytometry. View Larger

Detection of ICAM‑4 in Human Red Blood Cells by Flow Cytometry. Human red blood cells were stained with Mouse Anti-Human ICAM-4 APC-conjugated Monoclonal Antibody (Catalog # FAB8397A, filled histogram) or isotype control antibody (Catalog # IC0041A, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: ICAM-4

ICAM-4 (Intercellular Adhesion Molecule 4), also known as Landsteiner-Wiener Glycoprotein and CD242, is a 42 kDa member of the ICAM family, Ig superfamily of proteins. It is expressed on erythrocytes and erythroblasts, and serves as a receptor for LFA-1, Mac-1, and CD11c/CD18, plus alpha 4 beta 1 and alpha-V containing integrins. ICAM-4 is suggested to bind to Mac-1 on macrophages, allowing for its phagocytosis in senescence. Mature human ICAM-4 is a 249 amino acid (aa) type I transmembrane glycoprotein. It possesses a 218 aa extracellular region (aa 23-240) that contains two C2-type Ig-like domains (aa 62-124 and 146-217), and a 10 aa C-terminal cytoplasmic tail. ICAM-4 may form 85 kDa homodimers. There are three potential isoform variants. One shows a five aa substitution for aa 233-271, a second contains a 15 aa substitution for aa 14-29, and a third possesses a 141 aa substitution for aa 132-271. Over aa 31-240, human ICAM-4 shares 71% aa identity with mouse ICAM-4.

Long Name
Intercellular Adhesion Molecule 4
Entrez Gene IDs
3386 (Human); 78369 (Mouse)
Alternate Names
CD242 antigen; CD242; ICAM4; ICAM-4; intercellular adhesion molecule 4 (Landsteiner-Wiener blood group); intercellular adhesion molecule 4 (LW blood group); intercellular adhesion molecule 4; intercellular adhesion molecule 4, Landsteiner-Wiener blood group; Landsteiner-Wiener blood group antigen a; Landsteiner-Wiener blood group glycoprotein; Landsteiner-Wiener blood group; LWLW blood group protein

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