Human ICAM-4 Alexa Fluor® 594-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB8397T-025
Detection  of ICAM‑4 in Human Red Blood Cells by Flow Cytometry.
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Human ICAM-4 Alexa Fluor® 594-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human ICAM-4 in direct ELISAs.
Source
Monoclonal Mouse IgG2B Clone # 729632
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human ICAM-4
Ala23-Ala240
Accession # Q14773
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 594

Applications

Recommended Concentration
Sample
Flow Cytometry
5 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection  of ICAM‑4 in Human Red Blood Cells by Flow Cytometry. View Larger

Detection of ICAM‑4 in Human Red Blood Cells by Flow Cytometry. Human red blood cells were stained with Mouse Anti-Human ICAM‑4 Alexa Fluor® 594‑conjugated Monoclonal Antibody (Catalog # FAB8397T, filled histogram) or isotype control antibody (Catalog # IC0041T, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: ICAM-4

ICAM-4 (Intercellular Adhesion Molecule 4), also known as Landsteiner-Wiener Glycoprotein and CD242, is a 42 kDa member of the ICAM family, Ig superfamily of proteins. It is expressed on erythrocytes and erythroblasts, and serves as a receptor for LFA-1, Mac-1, and CD11c/CD18, plus alpha 4 beta 1 and alpha-V containing integrins. ICAM-4 is suggested to bind to Mac-1 on macrophages, allowing for its phagocytosis in senescence. Mature human ICAM-4 is a 249 amino acid (aa) type I transmembrane glycoprotein. It possesses a 218 aa extracellular region (aa 23-240) that contains two C2-type Ig-like domains (aa 62-124 and 146-217), and a 10 aa C-terminal cytoplasmic tail. ICAM-4 may form 85 kDa homodimers. There are three potential isoform variants. One shows a five aa substitution for aa 233-271, a second contains a 15 aa substitution for aa 14-29, and a third possesses a 141 aa substitution for aa 132-271. Over aa 31-240, human ICAM-4 shares 71% aa identity with mouse ICAM-4.

Long Name
Intercellular Adhesion Molecule 4
Entrez Gene IDs
3386 (Human); 78369 (Mouse)
Alternate Names
CD242 antigen; CD242; ICAM4; ICAM-4; intercellular adhesion molecule 4 (Landsteiner-Wiener blood group); intercellular adhesion molecule 4 (LW blood group); intercellular adhesion molecule 4; intercellular adhesion molecule 4, Landsteiner-Wiener blood group; Landsteiner-Wiener blood group antigen a; Landsteiner-Wiener blood group glycoprotein; Landsteiner-Wiener blood group; LWLW blood group protein

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