Interferon-alpha (IFN-alpha), also known as leukocyte interferon, represents a group of related but distinct proteins that share over 95% amino acid sequence homology. They are members of the type I interferon family which share a common cell surface receptor composed of two subunits, a 100 kDa ligand-binding subunit (IFN-alpha R2) and a 125 kDa ligand binding and signal transduction subunit (IFN-alpha R1) that is involved both in ligand binding and signal transduction. IFN-alpha has both anti-viral and immunomodulatory activities on target cells.
Human IFN-alpha 2 XL Luminex® Performance Assay
R&D Systems | Catalog # LUXLM9345B
Luminex High Performance Assays
Human IFN-alpha 2 XL Luminex® Performance Assay is specifically designed for multiplex detection of human IFN-alpha 2/IFNA2 with a panel of related analytes in serum, plasma, or tissue culture supernatants. Luminex High Performance Assays go through rigorous development, validation, and quality control testing to ensure the best performance of your multiplex assay.
This assay is customizable, select some or all the analytes in the entire panel. Customize your panel with the Luminex Assay Customization Tool.
Key Product Details
Panel
Human XL Cytokine Panel
Assay Type
Magnetic bead-based multiplex assay for the Luminex® platform
Format
1 x 96-well microplate and magnetic antibody-coated beads
Species
Human
Analytes Detected
IFN-alpha 2/IFNA2
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Product Features
Magnetic Luminex High Performance Assays are flexible bead-based multiplex assays. They allow up to 50 user-defined target analytes to be simultaneously profiled using cell culture supernates, serum, or plasma samples. Some panels are also validated with human milk, saliva, and/or urine samples.
- Simultaneously profile Human IFN-alpha 2/IFNA2 with a panel of related analytes in one sample
- Magnetic format allows for easier wash steps
- Undergoes similar validation testing as Quantikine® ELISA Kits
- Precise and Specific
- Requires a small sample volume (<50 µL)
- Run your assay in just 3-5 hours
- Mass-calibrated standards for consistent results with every new lot of material
- Enough reagents for one 96-well plate
- Quick turn-around time: Kits are typically shipped within 2-3 days of ordering*
*Please allow additional time for international orders.
Performance Validation
R&D Systems Luminex High Performance Assays undergo our most extensive validation testing. All Luminex High Performance Assay Panels are validated for use with serum and plasma samples. Some High Performance Assays are additionally validated for cell culture supernatants, milk, saliva, or urine, as indicated on the individual product data sheets. Luminex High Performance Assays are tested for sensitivity (three-quarters the low standard), intra-assay precision, inter-assay precision, and to ensure assay linearity for validated sample types. Recovery values for individual samples in validated sample types are also tested. Validation data for each analyte can be found in the product datasheet.
Base Kit Contents
- Premixed cocktail of biotinylated detection antibody
- Standard Cocktail(s)
- Bead Diluent
- Biotin Antibody Diluent
- Calibrator Diluent
- Wash Buffer
- Streptavidin-PE
- One flat-bottom 96-well Microplate
- Foil Plate Sealers (4)
- Mixing Bottle
- Standard Value Card**
- High and low controls with Control Mean Value Card
**A standard curve must be generated each time an assay is run, utilizing values from the Certificate of Analysis provided with each Luminex Assay.
Bead Set Contents
- User-mixed antibody-coated Magnetic beads in individual vials
Recommended Sample Types: Cell culture supernates, serum, EDTA plasma, and heparin plasma
Components: Human IFN-α Magnetic Microparticles (Part 898842) is supplied as a 100X concentrated stock (0.075 mL) with preservatives. Other Supplies Required: Magnetic Luminex® Performance Assay Human XL Discovery Base Kit (R&D Systems®, Catalog # LUXLM000).
Sensitivity: The Minimum Detectable Dose (MDD) was determined by adding two standard deviations to the mean MFI of twenty zero standard replicates and calculating the corresponding concentration. Six assays were evaluated, and the MDD of human IFN-α ranged from 0.198-0.721 pg/mL. The mean MDD was 0.291 pg/mL.
Specificity: This assay recognizes natural and recombinant human IFN-α.Recombinant human IFN-α/β R2 interferes at concentrations > 7.41 ng/mL.
Bead Region: 39
Precision
|
Intra-assay Precision (precision within an assay) - Two samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Inter-assay Precision (precision between assays) - Two samples of known concentration were tested in thirty separate assays to assess precision between assays. |
||||
| Intra-Assay Precision | Inter-Assay Precision | |||
|---|---|---|---|---|
| Sample | 1 | 2 | 1 | 2 |
| n | 20 | 20 | 30 | 30 |
| Mean (pg/mL) | 46.2 | 446 | 47.2 | 449 |
| Standard deviation | 1.56 | 16.6 | 6.17 | 31.3 |
| CV (%) | 37.6 | 3.7 | 13.0 | 97.4 |
Recovery
|
Samples containing and/or spiked with human IFN-alpha were evaluated for recovery. |
||||||
| Sample Type | Average % Recovery | Range (%) | ||||
|---|---|---|---|---|---|---|
| Cell culture supernate | 113 | 106-123 | ||||
| Serum | 87.3 | 81.6-97.6 | ||||
| EDTA plasma | 87.9 | 77.3-97.1 | ||||
| Heparin plasma | 89.3 | 82.6-95.3 | ||||
| Plaelet-poor EDTA | 91.3 | 80.4-101 | ||||
| Platelet-poor Heparin | 93.4 | 78.1-108 | ||||
Linearity
|
Samples containing and/or spiked with human IFN-alpha were serially diluted to evaluate assay linearity. |
||||||||||||
| Cell culture supernate | Serum | EDTA plasma | Heparin plasma | Platelet-poor EDTA | Platelet-poor Heparin | |||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 1:2 | Average % of Expected | 96.6 | 108 | 113 | 112 | 110 | 106 | |||||
| Range (%) | 91.4-100 | 97.0-115 | 104-119 | 105-122 | 104-115 | 98.4-114 | ||||||
| 1:4 | Average % of Expected | 91.3 | 103 | 111 | 105 | 111 | 108 | |||||
| Range (%) | 87.0-93.7 | 94.6-108 | 97.0-122 | 95.9-110 | 106-115 | 100-119 | ||||||
| 1:8 | Average % of Expected | 92.8 | 106 | 114 | 107 | 119 | 106 | |||||
| Range (%) | 87.3-96.6 | 97.4-116 | 97.2-124 | 95.2-116 | 109-138 | 95.5-121 | ||||||
| 1:16 | Average % of Expected | 92.4 | 103 | 109 | 101 | 117 | 101 | |||||
| Range (%) | 86.7-96.4 | 92.9-109 | 97.7-118 | 90.5-110 | 109-121 | 89.4-112 | ||||||
Which Luminex Assay is Right for Me?
Assays for the Luminex platform are offered as High Performance Assays or Discovery Assays.
High Performance Assays are optimized and validated as complete panels with a focused set of analytes. You can choose from Fixed Panels, Premixed options, or Configurable User Mix formats depending on your needs.
Discovery Assays deliver maximum flexibility, enabling the creation of fully customized panels tailored to your research needs. With universal reagents validated for compatibility across all analytes in the menu, they support endless customization possibilities.
Additional Information About Luminex Assays
All R&D Systems Luminex Assays are designed for use with any Luminex instrument including MAGPIX, Luminex 200, FLEXMAP 3D, and the INTELLIFLEX.
Luminex assays use color-coded, magnetic microparticles coated with analyte-specific antibodies to enable multiplex quantitation. During the assay, standards and samples are added to the wells, where target analytes bind to the capture antibodies. A biotinylated detection antibody cocktail is then introduced, followed by a Streptavidin-PE conjugate that generates a fluorescent signal proportional to the amount of analyte present.
Each microparticle is identified by a unique signature and the signal intensity is measured using a Luminex instrument. These assays are designed to deliver reliable and reproducible results with validated antibody pairs and optimized protocols. They are well suited for applications that require sensitivity, accuracy, and confidence in your data while enabling efficient multiplexing to measure multiple targets simultaneously.
Scientific Data Images for Human IFN-alpha 2 XL Luminex® Performance Assay
IFN-alpha 2 XL Cytokine Luminex Panel Standard Curves
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: IFN-alpha 2/IFNA2
Long Name
Interferon alpha 2
Alternate Names
Ifa2, IFNA2, IFNA2a, IFNA2b, IFNA2c, IFNAA, IFNalpha 2
Gene Symbol
IFNA2
Additional IFN-alpha 2/IFNA2 Products
Product Documents for Human IFN-alpha 2 XL Luminex® Performance Assay
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human IFN-alpha 2 XL Luminex® Performance Assay
Luminex® is a registered trademark of Luminex Corporation.
For research use only
Related Research Areas
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Associated Pathways
NOD-like Receptor Signaling Pathways
Toll-Like Receptor Signaling Pathways
