Human IL-17 RD/SEF Fluorescein-conjugated Antibody

FAB2275F has been discontinued. View all IL-17 RD/SEF products.
    
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  • Species Reactivity
    Human
  • Specificity
    Detects human IL‑17 RD/SEF in direct ELISAs and Western blots. In direct ELISAs, approximately 40% cross-reactivity with recombinant mouse (rm) IL-17 RD is observed and less than 10% cross-reactivity with recombinant human (rh) IL-17 RC, rmIL-17B R, and rhIL-17 R is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human IL‑17 RD/SEF
    Ala27-Arg299
    Accession # AAM77571
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Fluorescein
Product Datasheets

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Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Example
Detection of IL‑17 RD/SEF in K562 Human Cell Line by Flow Cytometry. K562 human chronic myelogenous leukemia cell line was stained with Goat Anti-Human
IL-17 RD/SEF Fluorescein‑conjugated Anti­gen Affinity-purified Polyclonal Anti­body (Catalog # FAB2275F, filled histogram) or isotype control antibody (Catalog # IC108F, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: IL-17 RD/SEF

Interleukin-17 receptor D (IL-17 RD), also known as SEF (similar expression to FGFs), is a type I transmembrane protein that is found in both the cytoplasm and plasma membrane (1-5). The gene for this protein belongs to a synexpression group originally identified in zebrafish where SEF is expressed along with FGF-3, -8, sprouty-2 (SPRY2) and SPRY4 (6, 7). By alternate splicing, two transcript variants, potentially encoding three protein isoforms, exist. One is a full-length long form, one a shortened form that uses an alternate start site, and one an alternate splice form that removes the classic signal sequence (1-4). These isoforms have different expression patterns, subcellular localization, and function. The membrane-bound long form of human IL-17RD is synthesized as a 739 amino acid (aa) precursor protein with a putative 27 aa signal peptide, a 272 aa extracellular domain, a 20 aa transmembrane segment and a 420 aa cytoplastic domain. The extracellular domain contains one Ig-like domain and a fibronectin type III motif. The cytoplasmic domain shares homology with the intracellular domains of IL-17 receptor family members and shows one TIR (Toll/IL-1 Receptor) domain and a putative TRAF6-binding motif (2). Natural IL-17 RD has been shown to form homomultimeric complexes (3). Unlike the alternate splice form of IL-17 RD that has a restricted pattern of expression, the full-length IL-17 RD isoform is expressed in most adult tissues and during embryonic development (3, 5). Functionally, IL-17 RD has been shown to be an inhibitor of FGF signaling. The molecule’s extracellular domain does not seem to be involved. There is an interaction between the intracellular domains of FGFR1/2 and IL-17 RD that blocks ERK dissociation from MEK, thereby interfering with downstream ERK activation of nuclear Elk-1 (8). IL-17 RD has also been reported to interact with TAK1 and induce JNK activation and apoptosis (9). Ligands that interact with the extracellular domain of IL-17 RD have not been identified.

  • References:
    1. Furthauer, M. et al. (2002) Nat. Cell Biol. 4:170.
    2. Xiong, S. et al. (2003) J. Biol. Chem. 278:50273.
    3. Yang, R-B. et al. (2003) J. Biol. Chem. 278:33232.
    4. Preger, E. et al. (2003) Proc. Natl. Acad. Sci. USA 101:1229.
    5. Lin, W. et al. (2002) Mech. Dev. 113:163.
    6. Tsang, M. et al. (2002) Nat. Cell Biol. 4:165.
    7. Kovalenko, D. et al. (2003) J. Biol. Chem. 278:14087.
    8. Torii, S. et al. (2004) Dev. Cell 7:33.
    9. Yang, X. et al. (2004) J. Biol. Chem. 279:38099.
  • Long Name:
    Interleukin 17 Receptor D, Transcript Variant 3
  • Entrez Gene IDs:
    54756 (Human); 171463 (Mouse)
  • Alternate Names:
    FLJ35755; IL-17 RD; IL17RD; IL-17RD; IL-17RDIL-17 receptor D; IL17Rhom; IL17RLM; IL17RLMDKFZp434N1928; interleukin 17 receptor D; interleukin-17 receptor D; Interleukin-17 receptor-like protein; MGC133309; Sef homolog; SEF; SEFhSef
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