Detects human Integrin beta 8 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human Integrins beta 1, beta 2, beta 3, beta 4, beta 5, beta 6 beta 7, or recombinant mouse Integrin beta 8 is observed.
Monoclonal Mouse IgG2B Clone # 416922
Protein A or G purified from hybridoma culture supernatant
Mouse myeloma cell line NS0-derived recombinant human Integrin beta 8 Glu43-Arg684 (predicted) Accession # P26012
Supplied in a saline solution containing BSA and Sodium Azide.
10 µL/106 cells
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Integrin beta 8 in U‑87 MG Human Cell Line by Flow Cytometry.
U‑87 MG human glioblastoma/astrocytoma cell line was stained with Mouse Anti-Human Integrin beta 8 APC‑conjugated Monoclonal Antibody (Catalog # FAB4775A, filled histogram) or isotype control antibody (Catalog # IC0041A, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
Background: Integrin beta 8
Integrin beta 8 (Integrin beta 8) is a 90 kDa type I transmembrane glycoprotein of the Integrin family of adhesion molecules. It associates with Integrin alpha V to form a receptor for vitronectin, fibrin, and the latency associated peptide (LAP). Binding to LAP promotes the proteolytic release of active TGF-beta from LAP. Integrin alpha V beta 8 is required for vascular morphogenesis in the embryonic brain and yolk sac. Within the extracellular domain, human Integrin beta 8 shares 87% aa sequence identity with mouse and rat Integrin beta 8.
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