Integrin beta 8 (Integrin beta 8) is a 90 kDa type I transmembrane glycoprotein of the Integrin family of adhesion molecules. It associates with Integrin alpha V to form a receptor for vitronectin, fibrin, and the latency associated peptide (LAP). Binding to LAP promotes the proteolytic release of active TGF-beta from LAP. Integrin alpha V beta 8 is required for vascular morphogenesis in the embryonic brain and yolk sac. Within the extracellular domain, human Integrin beta 8 shares 87% aa sequence identity with mouse and rat Integrin beta 8.
Human Integrin beta 8 APC‑conjugated Antibody
R&D Systems | Catalog # FAB4775A
Key Product Details
Species Reactivity
Human
Applications
Flow Cytometry
Label
Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)
Antibody Source
Monoclonal Mouse IgG2B Clone # 416922
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Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Integrin beta 8
Glu43-Arg684 (predicted)
Accession # P26012
Glu43-Arg684 (predicted)
Accession # P26012
Specificity
Detects human Integrin beta 8 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human Integrins beta 1, beta 2, beta 3, beta 4, beta 5, beta 6 beta 7, or recombinant mouse Integrin beta 8 is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human Integrin beta 8 APC‑conjugated Antibody
Detection of Integrin beta 8 in WM-115 Human Cell Line by Flow Cytometry.
WM-115 human melanoma cell line was stained with Mouse Anti-Human Integrin beta 8 APC-conjugated Monoclonal Antibody (Catalog # FAB4775A, filled histogram) or isotype control antibody (Catalog # IC0041A, open histogram). View our protocol for Staining Membrane-associated Proteins.Applications for Human Integrin beta 8 APC‑conjugated Antibody
Application
Recommended Usage
Flow Cytometry
10 µL/106 cells
Sample: WM-115 human melanoma cell line
Sample: WM-115 human melanoma cell line
Spectra Viewer
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
Spectra Viewer
Flow Cytometry Panel Builder
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Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.
Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: Integrin beta 8
Alternate Names
ITGB8
Gene Symbol
ITGB8
UniProt
Additional Integrin beta 8 Products
Product Documents for Human Integrin beta 8 APC‑conjugated Antibody
Certificate of Analysis
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Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human Integrin beta 8 APC‑conjugated Antibody
For research use only
Related Research Areas
Citations for Human Integrin beta 8 APC‑conjugated Antibody
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- 7-Amino Actinomycin D (7-AAD) Cell Viability Flow Cytometry Protocol
- Extracellular Membrane Flow Cytometry Protocol
- Flow Cytometry Protocol for Cell Surface Markers
- Flow Cytometry Protocol for Staining Membrane Associated Proteins
- Flow Cytometry Staining Protocols
- Flow Cytometry Troubleshooting Guide
- Intracellular Flow Cytometry Protocol Using Alcohol (Methanol)
- Intracellular Flow Cytometry Protocol Using Detergents
- Intracellular Nuclear Staining Flow Cytometry Protocol Using Detergents
- Intracellular Staining Flow Cytometry Protocol Using Alcohol Permeabilization
- Intracellular Staining Flow Cytometry Protocol Using Detergents to Permeabilize Cells
- Propidium Iodide Cell Viability Flow Cytometry Protocol
- Protocol for Liperfluo
- Protocol for the Characterization of Human Th22 Cells
- Protocol for the Characterization of Human Th9 Cells
- Protocol: Annexin V and PI Staining by Flow Cytometry
- Protocol: Annexin V and PI Staining for Apoptosis by Flow Cytometry
- Troubleshooting Guide: Fluorokine Flow Cytometry Kits
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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Associated Pathways
Blood-Brain Barrier Pathway: Anatomy
