Human Integrin  beta 8 Antibody

Catalog # Availability Size / Price Qty
MAB4775
MAB4775-SP

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Detection of Integrin  beta 8 in A172 Human Cell Line by Flow Cytometry.
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Citations (2)
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Human Integrin  beta 8 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Integrin beta 8 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human Integrins beta 1, beta 2, beta 3, beta 4, beta 5, beta 6, beta 7, or recombinant mouse Integrin beta 8 is observed.
Source
Monoclonal Mouse IgG2B Clone # 416922
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Integrin beta 8
Glu43-Arg684 (predicted)
Accession # P26012
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of Integrin  beta 8 antibody in A172 Human Cell Line antibody by Flow Cytometry. View Larger

Detection of Integrin beta 8 in A172 Human Cell Line by Flow Cytometry. WM-115 human melanoma cell line was stained with Human Integrin beta 8 Monoclonal Antibody (Catalog # MAB4775, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by APC-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Integrin beta 8

Integrin beta 8 (Integrin beta 8) is a 90 kDa type I transmembrane glycoprotein of the Integrin family of adhesion molecules. It associates with Integrin alpha V to form a receptor for vitronectin, fibrin, and the latency associated peptide (LAP). Binding to LAP promotes the proteolytic release of active TGF-beta from LAP. Integrin alpha V beta 8 is required for vascular morphogenesis in the embryonic brain and yolk sac. Within the extracellular domain, human Integrin  beta 8 shares 87% aa sequence identity with mouse and rat Integrin  beta 8.

Entrez Gene IDs
3696 (Human); 320910 (Mouse); 362800 (Rat)
Alternate Names
Integrin beta 8; integrin beta-8; integrin, beta 8; ITGB8

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Citations for Human Integrin  beta 8 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Integrin-alphaV-mediated activation of TGF-beta regulates anti-tumour CD8 T cell immunity and response to PD-1 blockade
    Authors: I Malenica, J Adam, S Corgnac, L Mezquita, E Auclin, I Damei, L Grynszpan, G Gros, V de Montpré, D Planchard, N Théret, B Besse, F Mami-Choua
    Nature Communications, 2021-09-01;12(1):5209.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Highly invasive melanoma cells activate the vascular endothelium via an MMP-2/integrin alphavbeta5-induced secretion of VEGF-A.
    Am. J. Pathol., 2012-05-31;181(2):693-705.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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