Human ISG15/UCRP Antibody

R&D Systems | Catalog # A-830

Product is in the process of being discontinued.
R&D Systems
Discontinued Product
A-830 has been discontinued. View all ISG15/UCRP products.

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, Immunoprecipitation

Cited:

Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 851701
View all ISG15/UCRP Primary Antibodies »

Product Specifications

Immunogen

Full-length recombinant human ISG15/UCRP
Accession # P05161

Specificity

This antibody will recover ISGylated proteins in immunoprecipitation applications. This antibody detects ISGylated proteins in Western blots.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Scientific Data Images for Human ISG15/UCRP Antibody

Immunoprecipitation of Human ISG15/UCRP.

Immunoprecipitation of Human ISG15/UCRP.

A549 cells transfected with HA-ISG15 were treated briefly with IFN gamma or carrier only. 500 µg of cell lysates were subjected to immunoprecipitation (IP) with 20 µg A-830 using Protein G Sepharose. IP’d material was resolved on reducing SDS-PAGE gels and Western Blots were developed using PVDF membranes and anti-HA epitope (R&D Systems MAB060) at 0.5 µg/ml followed by HRP-labeled anti-mouse (R&D Systems # HAF007) secondary antibody at 1:2000 dilution. Free (unconjugated) HA-ISG15 was visualized in both treated and untreated cells, while ISGylated proteins were clearly recovered (IP’d) from the interferon-treated cell lysates.

Applications for Human ISG15/UCRP Antibody

Application
Recommended Usage

Immunoprecipitation

Recommended IP conditions: use 10-20 µg of A-830 to recover ISGylated proteins from 0.5 – 1 mg of total protein in 1 ml of cellular lysate. Recommended concentration for Western Blot is 0.1 - 0.5 µg/ml.

Western Blot

0.1-0.5 µg/mL
Sample: A549 cells transfected with HA-ISG15 treated with IFN-gamma

Reviewed Applications

Read 2 reviews rated 5 using A-830 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied as a solution in PBS containing Glycerol and Sodium Azide.

Shipping

The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at -20 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Storage below -20 °C is not recommended. Do not use past expiration date.

Background: ISG15/UCRP

Interferon­stimulated Gene 15 (ISG15), also known as Ubiquitin Cross­reacting Protein (UCRP), is a Ubiquitin­like protein that is covalently coupled to target proteins in a process termed ISGylation. It is a 165 amino acid (aa) polypeptide with a predicted molecular weight of 18 kDa. ISG15/UCRP exhibits 66% aa sequence identity with its mouse ortholog. Structurally, ISG15/UCRP consists of two tandem Ubiquitin­like domains that share a similar 3­dimensional structure with Ubiquitin and other Ubiquitin­like modifiers including NEDD8 and SUMO1. Modification of targets by ISG15/UCRP occurs in a stepwise enzymatic process similar to that of Ubiquitin. Enzymes regulating ISGylation include the activating (E1) enzyme UBE1L, the conjugating (E2) enzyme UbcH8, and ligases (E3) such as HERC5. Removal of ISG15/UCRP is catalyzed by the deconjugating enzyme UBP43/USP18. Functionally, ISG15/UCRP has putative roles in the immune response and tumorigenesis. This is reflected by intracellular ISG15/UCRP targets that include Cyclin D1, tumor suppressor p63, IRF3, and a range of viral proteins. It is induced by type 1 interferons and microbial infection, and knockout mice exhibit an increased sensitivity to infection by some viruses. ISG15/UCRP can also be secreted by cells of the immune system and may act in a cytokine­like manner. For instance, it is produced by human granulocytes in response to mycobacterium exposure, and natural killer cells and T cells respond to extracellular ISG15/UCRP with IFN­gamma production. Further supporting a role in immune function, ISG15/UCRP mutations are associated with MSMD, an inherited disorder characterized by increased susceptibility to mycobacterial infection.

Long Name

ISG15 Ubiquitin-like Modifier

Alternate Names

G1P2, IFI15, IP17, UCRP

Entrez Gene IDs

9636 (Human); 100038882 (Mouse); 298693 (Rat)

Gene Symbol

ISG15

UniProt

P05161 (Human)

Additional ISG15/UCRP Products

Product Documents for Human ISG15/UCRP Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human ISG15/UCRP Antibody

* Contains <0.1% Sodium Azide, which is not hazardous at this concentration according to GHS classifications. Refer to SDS for additional information and handling instructions.

For research use only

Citations for Human ISG15/UCRP Antibody

Customer Reviews for Human ISG15/UCRP Antibody (2)

5 out of 5
2 Customer Ratings
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1 Stars
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  • Human ISG15/UCRP Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: Human embryonic kidney 293T cells
    Species: Human
    Verified Customer | Posted 09/30/2021
    Human ISG15/UCRP Antibody A-830
  • Human ISG15/UCRP Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: HT1080 human fibrosarcoma cell line
    Species: Human
    Verified Customer | Posted 08/05/2021
    Human ISG15/UCRP Antibody A-830

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