Human ITFG1 APC-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB89001A-025
Detection  of ITFG1 in K562 Human Cell Line by Flow Cytometry.
1 Image
Product Details
FAQs
Supplemental Products
Reviews

Human ITFG1 APC-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human ITFG1 in direct ELISAs.
Source
Monoclonal Mouse IgG2B Clone # 936213
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Human embryonic kidney cell line HEK293-derived human ITFG1
Met1-Ile566
Accession # Q8TB96
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Allophycocyanin

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection  of ITFG1 in K562 Human Cell Line by Flow Cytometry. View Larger

Detection of ITFG1 in K562 Human Cell Line by Flow Cytometry. K562 human chronic myelogenous leukemia cell line was stained with Mouse Anti-Human ITFG1 APC‑conjugated Monoclonal Antibody (Catalog # FAB89001A, filled histogram) or isotype control antibody (Catalog # IC0041A, open histogram). View our protocol for Staining Membrane-associated Proteins

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: ITFG1

Integrin-alpha FG-GAP repeat-containing protein 1 (ITFG1), also known as T cell immunomodulatory protein (TIP), was initially identified using bioinformatics and high-throughput cell-based screening assays to isolate novel factors involved in T cell biology.  Ubiquitously expressed as a 96-100 kDa glycoprotein (it is absent on T cells, B cells, and monocytes), ITFG1 would appear to be a type I transmembrane protein that contains a 534 amino acids (aa) N-terminal ECD coupled to a 45 aa C-terminal transmembrane and cytoplasmic domain. Both soluble and transmembrane forms of the ITFG1 appear to exist. Human and mouse T cells treated with ITFG1 in vitro secreted the cytokines IFN-gamma, TNF-alpha and IL-10, while in vivo, ITFG1 was protective in a mouse acute graft-versus-host disease (GVHD) model. Based on invertebrate models, human ITFG1 likely serves as a cell adhesion molecule under certain circumstances. Over aa 1-566, human ITFG1 shares 89% and 88% aa sequence identity with mouse and rat ITFG1, respectively.

Long Name
Integrin Alpha FG-GAP Repeat Containing 1
Entrez Gene IDs
81533 (Human); 71927 (Mouse); 171083 (Rat)
Alternate Names
CDA08; integrin alpha FG-GAP repeat containing 1,2310047C21Rik; Integrin-alpha FG-GAP repeat-containing protein 1; ITFG1; Protein TIP; T-cell immunomodulatory protein; TIP; TIPT cell immunomodulatory protein

Product Datasheets

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Reviews for Human ITFG1 APC-conjugated Antibody

There are currently no reviews for this product. Be the first to review Human ITFG1 APC-conjugated Antibody and earn rewards!

Have you used Human ITFG1 APC-conjugated Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review