Detection of ITFG1 in K562 Human Cell Line by Flow Cytometry. K562 human chronic myelogenous leukemia cell line was stained with Mouse Anti-Human ITFG1 APC‑conjugated Monoclonal Antibody (Catalog # FAB89001A, filled histogram) or isotype control antibody (Catalog # IC0041A, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
Integrin-alpha FG-GAP repeat-containing protein 1 (ITFG1), also known as T cell immunomodulatory protein (TIP), was initially identified using bioinformatics and high-throughput cell-based screening assays to isolate novel factors involved in T cell biology. Ubiquitously expressed as a 96-100 kDa glycoprotein (it is absent on T cells, B cells, and monocytes), ITFG1 would appear to be a type I transmembrane protein that contains a 534 amino acids (aa) N-terminal ECD coupled to a 45 aa C-terminal transmembrane and cytoplasmic domain. Both soluble and transmembrane forms of the ITFG1 appear to exist. Human and mouse T cells treated with ITFG1 in vitro secreted the cytokines IFN-gamma, TNF-alpha and IL-10, while in vivo, ITFG1 was protective in a mouse acute graft-versus-host disease (GVHD) model. Based on invertebrate models, human ITFG1 likely serves as a cell adhesion molecule under certain circumstances. Over aa 1-566, human ITFG1 shares 89% and 88% aa sequence identity with mouse and rat ITFG1, respectively.
Integrin Alpha FG-GAP Repeat Containing 1
Entrez Gene IDs:
81533 (Human); 71927 (Mouse); 171083 (Rat)
CDA08; integrin alpha FG-GAP repeat containing 1,2310047C21Rik; Integrin-alpha FG-GAP repeat-containing protein 1; Protein TIP; T-cell immunomodulatory protein; TIP; TIPT cell immunomodulatory protein
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