Human ITFG1 APC-conjugated Antibody

Catalog # Availability Size / Price Qty
  Detection  of ITFG1 in K562 Human Cell Line by Flow Cytometry.
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Product Details
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Human ITFG1 APC-conjugated Antibody Summary

Species Reactivity
Detects human ITFG1 in direct ELISAs.
Monoclonal Mouse IgG2B Clone # 936213
Protein A or G purified from hybridoma culture supernatant
Human embryonic kidney cell line HEK293-derived human ITFG1
Accession # Q8TB96
Supplied in a saline solution containing BSA and Sodium Azide.


Recommended Concentration
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection  of ITFG1 in K562 Human Cell Line by Flow Cytometry. View Larger

Detection of ITFG1 in K562 Human Cell Line by Flow Cytometry. K562 human chronic myelogenous leukemia cell line was stained with Mouse Anti-Human ITFG1 APC‑conjugated Monoclonal Antibody (Catalog # FAB89001A, filled histogram) or isotype control antibody (Catalog # IC0041A, open histogram). View our protocol for Staining Membrane-associated Proteins

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Preparation and Storage

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: ITFG1

Integrin-alpha FG-GAP repeat-containing protein 1 (ITFG1), also known as T cell immunomodulatory protein (TIP), was initially identified using bioinformatics and high-throughput cell-based screening assays to isolate novel factors involved in T cell biology.  Ubiquitously expressed as a 96-100 kDa glycoprotein (it is absent on T cells, B cells, and monocytes), ITFG1 would appear to be a type I transmembrane protein that contains a 534 amino acids (aa) N-terminal ECD coupled to a 45 aa C-terminal transmembrane and cytoplasmic domain. Both soluble and transmembrane forms of the ITFG1 appear to exist. Human and mouse T cells treated with ITFG1 in vitro secreted the cytokines IFN-gamma, TNF-alpha and IL-10, while in vivo, ITFG1 was protective in a mouse acute graft-versus-host disease (GVHD) model. Based on invertebrate models, human ITFG1 likely serves as a cell adhesion molecule under certain circumstances. Over aa 1-566, human ITFG1 shares 89% and 88% aa sequence identity with mouse and rat ITFG1, respectively.

Long Name
Integrin Alpha FG-GAP Repeat Containing 1
Entrez Gene IDs
81533 (Human); 71927 (Mouse); 171083 (Rat)
Alternate Names
CDA08; integrin alpha FG-GAP repeat containing 1,2310047C21Rik; Integrin-alpha FG-GAP repeat-containing protein 1; ITFG1; Protein TIP; T-cell immunomodulatory protein; TIP; TIPT cell immunomodulatory protein

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