Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Neutralization, Immunoprecipitation

Cited:

Neutralization

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG1 Clone # 369910
View all Kallikrein 8/Neuropsin Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Kallikrein 8/Neuropsin
Gln29-Gly260 (predicted)
Accession # O60259

Specificity

Detects human Kallikrein 8/Neuropsin in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) UPA‑1, rhKallikrein 1, 2, 3, 4, 5, 7, 9, 10, 11, 12, 13, 14, 15, rhKallikrein B1, rhHGFA, rhFactor VII, rhFactor X, rhFactor XI, rhProc, or rhThrombin is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG1

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Applications for Human Kallikrein 8/Neuropsin Antibody

Application
Recommended Usage

Immunoprecipitation

25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human Kallikrein 8/Neuropsin (Catalog # 2025-SE), see our available Western blot detection antibodies

Neutralization

Measured by its ability to neutralize Recombinant Human Kallikrein 8/Neuropsin (0.5 µg/mL, Catalog # 2025-SE) cleavage of the fluorogenic peptide substrate Boc-VPR-Amc (0.1 mM, Catalog # ES011). The Neutralization Dose (ND50) is typically 0.7 µg/mL.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Kallikrein 8/Neuropsin

Kallikrein 8 (KLK8), also known as neuropsin or ovasin, is a member of the human tissue kallikrein family (1). Two alternatively spliced forms exist, resulting in 260 (isoform 1) and 305 (isoform 2) amino acid (aa) sequences, respectively (2). Isoform 1 consists of a signal peptide (residues 1 to 28), a short pro peptide (aa 29‑32) and the mature chain (aa 33-260). Isoform 2 is identical to isoform 1, except that a 45 aa segment is inserted in isoform 2 between aa 23 and 24 in isoform 1. Isoform 1 is predominantly expressed in pancreas whereas isoform 2 is preferentially expressed in adult brain and hippocampus, although both forms are expressed in fetal brain and placenta in comparable levels. The brain function of KLK8 seems evident in neuropsin knockout mice that showed abnormalities of synapses and neurons and predisposation to global seizure activity (3, 4). KLK8 is a novel marker for ovarian and cervical cancer carcinomas (5, 6). Recombinant human KLK8, after being activated by lysyl endopeptidase, can cleave fibronectin and several small peptide substrates (7, 8).

References

  1. Yousef, G.M. and E.P. Diamandis (2001) Endocrine Rev. 22:184.
  2. Mitsui, S. et al. (1999) Eur. J. Biochem. 260:627.
  3. Hirata, A. et al. (2001) Mol. Cell. Neurosci. 17:600.
  4. Davies, B. et al. (2001) J. Neurosci. 21:6993.
  5. Kishi, T. et al. (2003) Cancer Res. 63:2771.
  6. Cane, S. et al. (2004) Am. J. Obstet. Gynecol. 190:60.
  7. Oka, T. et al. (2002) J. Biol. Chem. 277:14724.
  8. Shimizu, C. et al. (1998) J. Biol. Chem. 273:11189.

Alternate Names

KLK8, Neuropsin, Ovasin, PRSS19

Entrez Gene IDs

11202 (Human)

Gene Symbol

KLK8

UniProt

O60259

Additional Kallikrein 8/Neuropsin Products

Product Documents for Human Kallikrein 8/Neuropsin Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Kallikrein 8/Neuropsin Antibody

For research use only

Citations for Human Kallikrein 8/Neuropsin Antibody

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Protocols

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