Goat Anti-Rat IgG Antibody

Catalog # Availability Size / Price Qty
AF005
Product Details
Citations (2)
FAQs
Supplemental Products
Reviews (1)

Goat Anti-Rat IgG Antibody Summary

Species Reactivity
Rat
Specificity
Detects rat IgG1, IgG2A, IgG2B heavy and light chains.  In direct ELISA, less than 10% cross-reactivity with mouse IgG and less than 1% cross-reactivity with human IgG and rabbit IgG is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Rat IgG
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot

Use at a 1:5000 dilution in conjunction with an appropriate tertiary detection antibody

 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IgG

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name
Immunoglobulin G
Alternate Names
IgG; immunoglobulin heavy constant gamma 1 (G1m marker)

Product Datasheets

Citations for Goat Anti-Rat IgG Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Intravascular hemolysis activates complement via cell-free heme and heme-loaded microvesicles
    Authors: NS Merle, A Grunenwald, H Rajaratnam, V Gnemmi, M Frimat, ML Figueres, S Knockaert, S Bouzekri, D Charue, R Noe, T Robe-Rybki, M Le-Hoang, N Brinkman, T Gentinetta, M Edler, S Petrillo, E Tolosano, S Miescher, S Le Jeune, P Houillier, S Chauvet, M Rabant, JD Dimitrov, V Fremeaux-B, OP Blanc-Brud, LT Roumenina
    JCI Insight, 2018;3(12):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  2. Comparison of the roles of IL-1, IL-6, and TNFalpha in cell culture and murine models of aseptic loosening.
    Authors: Taki N, Tatro JM, Lowe R, Goldberg VM, Greenfield EM
    Bone, 2007;40(5):1276-83.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Ctrl

FAQs

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Isotype Controls

Secondary Antibodies

Reviews for Goat Anti-Rat IgG Antibody

Average Rating: 4 (Based on 1 Review)

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Goat Anti-Rat IgG Antibody
By Anonymous on 05/01/2018
Application: IHC Sample Tested: Cancer Tissue Species: Rat