hh Rat IgG1 Isotype Control MAB005: R&D Systems

Rat IgG1 Isotype Control

(37 citations)   
  • Specificity
    Selected for use as rat IgG1 isotype control. In direct ELISAs, no cross-react with recombinant human (rh) 6Ckine, recombinant mouse (rm) 6Ckine, rhACT II (rhMIP-1 beta ), rhBLC/BCA-1, rmC10, recombinant rat (rr) CINC-1, rrCINC-2 alpha, rrCINC-2 beta, rmCRG-2, rhENA-78, rhEotaxin, rmEotaxin, rhEotaxin 2, rhFractalkine, rmFractalkine, rhGCP-2, rmGCP-2, rhGRO alpha, rhGRO beta, rhGRO gamma, rhHCC-1, rhHCC-4, rhI-309, rhIL-8, rhIL-16, rhIP-10, rmJE, rmKC, rmLymphotactin, rmMARC, rhMCP-1, rhMCP-1 R, rhMCP-2, rhMCP-3, rhMCP-4, rmMCP-5, rhMDC, rmMDC, rhMIG, rmMIG, rhMIP-1 alpha, rmMIP-1 alpha, rmMIP-1 beta, rmMIP-1 gamma, rhMIP-1δ, rmMIP-2, rhMIP-3 alpha, rrMIP-3 alpha, rhMIP-3 beta, rmMIP-3 beta, recombinant viral (rv) MIP-I, rvMIP-II, rvMIP-III, rhMPIF-1, rhNAP-2, rhParc, rhRANTES, rmRANTES, rhSDF-1 alpha, rmSDF-1 alpha, rhSDF-1 beta, rhTarc, rmTCA-3, rhTeck, or rmTeck.
  • Source
    Monoclonal Rat IgG1 Clone # 43414
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    KLH
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Control
    Negative control for use in conjunction with R&D Systems antibodies. Use at the same concentration as the detection antibody.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Rat IgGControl by Flow Cytometry Mouse platelets were stained with Rat Anti-Mouse GPVIMonoclonal Antibody (Catalog # MAB6758,filled histogram) or Rat IgG Isotype Control Antibody (Catalog # MAB005,open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG SecondaryAntibody (Catalog # F0113).
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IgG1

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

  • Long Name:
    Immunoglobulin G1
  • Entrez Gene IDs:
    101867415 (Cynomolgus Monkey)
  • Alternate Names:
    IgG1
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

37 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. Granulocyte colony-stimulating factor blockade enables dexamethasone to inhibit lipopolysaccharide-induced murine lung neutrophils
    Authors: J Banuelos, Y Cao, SC Shin, BS Bochner, P Avila, S Li, X Jiang, MW Lingen, RP Schleimer, NZ Lu
    PLoS ONE, 2017;12(5):e0177884.
    Species: Mouse
    Sample Type: In Vivo
    Application: Neut
  2. Cooperation of Gastric Mononuclear Phagocytes with Helicobacter pylori during Colonization
    Authors: M Viladomiu, J Bassaganya, N Tubau-Juni, B Kronsteine, A Leber, CW Philipson, V Zoccoli-Ro, R Hontecilla
    J. Immunol, 2017;0(0):.
    Species: Mouse
    Sample Type: In Vivo
    Application: In Vivo
  3. Induction of necroptotic cell death by viral activation of the RIG-I or STING pathway
    Authors: SN Schock, NV Chandra, Y Sun, T Irie, Y Kitagawa, B Gotoh, L Coscoy, A Winoto
    Cell Death Differ, 2017;0(0):.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  4. IL6 Blockade Reprograms the Lung Tumor Microenvironment to Limit the Development and Progression of K-ras-Mutant Lung Cancer
    Authors: MS Caetano, H Zhang, AM Cumpian, L Gong, N Unver, EJ Ostrin, S Daliri, SH Chang, CE Ochoa, S Hanash, C Behrens, II Wistuba, C Sternberg, H Kadara, CG Ferreira, SS Watowich, SJ Moghaddam
    Cancer Res., 2016;76(11):3189-99.
    Species: Mouse
    Sample Type: In Vivo
    Application: Neut
  5. Role of G-CSF in monophosphoryl lipid A-mediated augmentation of neutrophil functions after burn injury.
    Authors: Bohannon J, Luan L, Hernandez A, Afzal A, Guo Y, Patil N, Fensterheim B, Sherwood E
    J Leukoc Biol, 2016;99(4):629-40.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  6. Presentation of Cryptic Peptides by MHC Class I Is Enhanced by Inflammatory Stimuli
    J Immunol, 2016;0(0):.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Neut
  7. Coexistent ARID1A-PIK3CA mutations promote ovarian clear-cell tumorigenesis through pro-tumorigenic inflammatory cytokine signalling.
    Authors: Chandler, Ronald L, Damrauer, Jeffrey, Raab, Jesse R, Schisler, Jonathan, Wilkerson, Matthew, Didion, John P, Starmer, Joshua, Serber, Daniel, Yee, Della, Xiong, Jessie, Darr, David B, Pardo-Manuel de Villena, Fernando, Kim, William, Magnuson, Terry
    Nat Commun, 2015;6(0):6118.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Neut Ctrl
  8. Neuropathic pain is constitutively suppressed in early life by anti-inflammatory neuroimmune regulation.
    Authors: McKelvey R, Berta T, Old E, Ji R, Fitzgerald M
    J Neurosci, 2015;35(2):457-66.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  9. G-CSF Promotes Neuroblastoma Tumorigenicity and Metastasis via STAT3-Dependent Cancer Stem Cell Activation.
    Authors: Agarwal S, Lakoma A, Chen Z, Hicks J, Metelitsa L, Kim E, Shohet J
    Cancer Res, 2015;75(12):2566-79.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  10. Mechanisms of Hypoxic Up-Regulation of Versican Gene Expression in Macrophages.
    Authors: Sotoodehnejadnematalahi F, Staples K, Chrysanthou E, Pearson H, Ziegler-Heitbrock L, Burke B
    PLoS ONE, 2015;10(6):e0125799.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  11. Critical role for IL-18 in spontaneous lung inflammation caused by autophagy deficiency.
    Authors: Abdel Fattah E, Bhattacharya A, Herron A, Safdar Z, Eissa N
    J Immunol, 2015;194(11):5407-16.
    Species: Mouse
    Sample Type: In Vivo
    Application: Neut Ctrl
  12. Cessation of CCL2 inhibition accelerates breast cancer metastasis by promoting angiogenesis.
    Authors: Bonapace L, Coissieux M, Wyckoff J, Mertz K, Varga Z, Junt T, Bentires-Alj M
    Nature, 2015;515(7525):130-3.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  13. Apolipoprotein E promotes subretinal mononuclear phagocyte survival and chronic inflammation in age-related macular degeneration.
    Authors: Levy O, Calippe B, Lavalette S, Hu S, Raoul W, Dominguez E, Housset M, Paques M, Sahel J, Bemelmans A, Combadiere C, Guillonneau X, Sennlaub F
    EMBO Mol Med, 2015;7(2):211-26.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo injection
  14. Coordinate expansion of murine hematopoietic and mesenchymal stem cell compartments by SHIPi.
    Authors: Brooks R, Iyer S, Akada H, Neelam S, Russo C, Chisholm J, Kerr W
    Stem Cells, 2015;33(3):848-58.
    Species: Mouse
    Sample Type: In Vivo
    Application: Neut Ctrl
  15. Netrin-1 promotes adipose tissue macrophage retention and insulin resistance in obesity.
    Authors: Ramkhelawon B, Hennessy E, Menager M, Ray T, Sheedy F, Hutchison S, Wanschel A, Oldebeken S, Geoffrion M, Spiro W, Miller G, McPherson R, Rayner K, Moore K
    Nat Med, 2014;20(4):377-84.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay - Ctrl
  16. Catecholamine stress alters neutrophil trafficking and impairs wound healing by beta2-adrenergic receptor-mediated upregulation of IL-6.
    Authors: Kim M, Gorouhi F, Ramirez S, Granick J, Byrne B, Soulika A, Simon S, Isseroff R
    J Invest Dermatol, 2014;134(3):809-17.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  17. Targeting DNGR-1 (CLEC9A) with antibody/MUC1 peptide conjugates as a vaccine for carcinomas.
    Authors: Picco G, Beatson R, Taylor-Papadimitriou J, Burchell J
    Eur J Immunol, 2014;44(7):1947-55.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  18. Retinoic acid prevents mesenteric lymph node dendritic cells from inducing IL-13-producing inflammatory Th2 cells.
    Authors: Yokota-Nakatsuma A, Takeuchi H, Ohoka Y, Kato C, Song S, Hoshino T, Yagita H, Ohteki T, Iwata M
    Mucosal Immunol, 2014;7(4):786-801.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Isotype control
  19. Leukocyte attraction by CCL20 and its receptor CCR6 in humans and mice with pneumococcal meningitis.
    Authors: Klein M, Brouwer M, Angele B, Geldhoff M, Marquez G, Varona R, Hacker G, Schmetzer H, Hacker H, Hammerschmidt S, van der Ende A, Pfister H, van de Beek D, Koedel U
    PLoS ONE, 2014;9(4):e93057.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  20. G-CSF drives a posttraumatic immune program that protects the host from infection.
    Authors: Gardner J, Noel J, Nikolaidis N, Karns R, Aronow B, Ogle C, McCormack F
    J Immunol, 2014;192(5):2405-17.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  21. The role of host and microbial factors in the pathogenesis of pneumococcal bacteraemia arising from a single bacterial cell bottleneck.
    Authors: Gerlini A, Colomba L, Furi L, Braccini T, Manso A, Pammolli A, Wang B, Vivi A, Tassini M, Van Rooijen N, Pozzi G, Ricci S, Andrew P, Koedel U, Moxon E, Oggioni M
    PLoS Pathog, 2014;10(3):e1004026.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  22. Syndecan-1 in the mouse parietal peritoneum microcirculation in inflammation.
    Authors: Kowalewska P, Patrick A, Fox-Robichaud A
    PLoS ONE, 2014;9(9):e104537.
    Species: Mouse
    Sample Type: In Vivo
    Application: IHC Fresh
  23. Acute lung injury and acute kidney injury are established by four hours in experimental sepsis and are improved with pre, but not post, sepsis administration of TNF-alpha antibodies.
    Authors: Bhargava R, Altmann C, Andres-Hernando A, Webb R, Okamura K, Yang Y, Falk S, Schmidt E, Faubel S
    PLoS ONE, 2013;8(11):e79037.
    Species: Mouse
    Sample Type: In Vivo
    Application: Isotype control
  24. CCR6 is required for epidermal trafficking of gammadelta-T cells in an IL-23-induced model of psoriasiform dermatitis.
    Authors: Mabuchi T, Singh T, Takekoshi T, Jia G, Wu X, Kao M, Weiss I, Farber J, Hwang S
    J Invest Dermatol, 2013;133(1):164-71.
    Species: Mouse
    Sample Type: In Vivo
    Application: Neut Ctrl
  25. Canonical and noncanonical Hedgehog pathway in the pathogenesis of multiple myeloma.
    Authors: Blotta, Simona, Jakubikova, Jana, Calimeri, Teresa, Roccaro, Aldo M, Amodio, Nicola, Azab, Abdel Ka, Foresta, Umberto, Mitsiades, Constant, Rossi, Marco, Todoerti, Katia, Molica, Stefano, Morabito, Fortunat, Neri, Antonino, Tagliaferri, Piersand, Tassone, Pierfran, Anderson, Kenneth, Munshi, Nikhil C
    Blood, 2012;120(25):5002-13.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow control
  26. Tumor-derived G-CSF facilitates neoplastic growth through a granulocytic myeloid-derived suppressor cell-dependent mechanism.
    Authors: Waight JD, Hu Q, Miller A, Liu S, Abrams SI
    PLoS ONE, 2011;6(11):e27690.
    Species: Mouse
    Sample Type: In Vivo
    Application: Ctrl
  27. GDF-15 is an inhibitor of leukocyte integrin activation required for survival after myocardial infarction in mice.
    Authors: Kempf T, Zarbock A, Widera C, Butz S, Stadtmann A, Rossaint J, Bolomini-Vittori M, Korf-Klingebiel M, Napp LC, Hansen B, Kanwischer A, Bavendiek U, Beutel G, Hapke M, Sauer MG, Laudanna C, Hogg N, Vestweber D, Wollert KC
    Nat. Med., 2011;17(5):581-8.
    Species: Mouse
    Sample Type: In Vivo
    Application: Ctrl
  28. Homeostatic imbalance of regulatory and effector T cells due to IL-2 deprivation amplifies murine lupus.
    Authors: Humrich JY, Morbach H, Undeutsch R, Enghard P, Rosenberger S, Weigert O, Kloke L, Heimann J, Gaber T, Brandenburg S, Scheffold A, Huehn J, Radbruch A, Burmester GR, Riemekasten G
    Proc. Natl. Acad. Sci. U.S.A., 2010;107(1):204-9.
    Species: Mouse
    Sample Type: In Vivo
    Application: Ctrl
  29. G-CSF-initiated myeloid cell mobilization and angiogenesis mediate tumor refractoriness to anti-VEGF therapy in mouse models.
    Authors: Shojaei F, Wu X, Qu X, Kowanetz M, Yu L, Tan M, Meng YG, Ferrara N
    Proc. Natl. Acad. Sci. U.S.A., 2009;106(16):6742-7.
    Species: Mouse
    Sample Type: In Vivo
    Application: Ctrl
  30. Rapid dendritic cell mobilization to the large intestinal epithelium is associated with resistance to Trichuris muris infection.
    Authors: Cruickshank SM, Deschoolmeester ML, Svensson M, Howell G, Bazakou A, Logunova L, Little MC, English N, Mack M, Grencis RK, Else KJ, Carding SR
    J. Immunol., 2009;182(5):3055-62.
    Species: Mouse
    Sample Type: In Vivo
    Application: Ctrl
  31. Tr1 regulatory T cells induced by ConA pretreatment prevent mice from ConA-induced hepatitis.
    Authors: Ye F, Yan S, Xu L, Jiang Z, Liu N, Xiong S, Wang Y, Chu Y
    Immunol. Lett., 2009;122(2):198-207.
    Species: Mouse
    Sample Type: In Vivo
    Application: Ctrl
  32. IL-23 is required for neutrophil homeostasis in normal and neutrophilic mice.
    Authors: Smith E, Zarbock A, Stark MA, Burcin TL, Bruce AC, Foley P, Ley K
    J. Immunol., 2007;179(12):8274-9.
    Species: Mouse
    Sample Type: In Vivo
    Application: Ctrl
  33. Anti-type II collagen antibody accelerates arthritis via CXCR2-expressing cells in IL-1 receptor antagonist-deficient mice.
    Authors: Kagari T, Tanaka D, Doi H, Iwakura Y, Shimozato T
    Eur. J. Immunol., 2007;37(10):2753-63.
    Species: Mouse
    Sample Type: In Vivo
    Application: Ctrl
  34. Junctional adhesion molecules (JAM)-B and -C contribute to leukocyte extravasation to the skin and mediate cutaneous inflammation.
    Authors: Ludwig RJ, Zollner TM, Santoso S, Hardt K, Gille J, Baatz H, Johann PS, Pfeffer J, Radeke HH, Schon MP, Kaufmann R, Boehncke WH, Podda M
    J. Invest. Dermatol., 2005;125(5):969-76.
    Species: Mouse
    Sample Type: In Vivo
    Application: Ctrl
  35. MIP-1 gamma promotes receptor-activator-of-NF-kappa-B-ligand-induced osteoclast formation and survival.
    Authors: Okamatsu Y, Battaglino R, Spate U, Stashenko P
    J. Immunol., 2004;173(3):2084-90.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Neut
  36. MIP-1 gamma promotes receptor-activator-of-NF-kappa-B-ligand-induced osteoclast formation and survival.
    Authors: Okamatsu Y, Battaglino R, Spate U, Stashenko P
    J. Immunol., 2004;173(3):2084-90.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Ctrl
  37. Granulocyte colony-stimulating factor antibody abrogates radioprotective efficacy of gamma-tocotrienol, a promising radiation countermeasure.
    Authors: Kulkarni, Shilpa, Singh, Pankaj K, Ghosh, Sanchita, Posarac, Ana, Singh, Vijay K
    Cytokine, 213;62(2):278-85.
    Species: Mouse
    Sample Type: In Vivo
Expand to show all 37 Citations
Isotype Controls
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Rat IgG1 PE-conjugated Antibody

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Rat IgG1 APC-conjugated Isotype Control

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Rat IgG1 Alexa Fluor® 647-conjugated Antibody

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Rat IgG1 Alexa Fluor® 488-conjugated Isotype Control

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Rat IgG1 Alexa Fluor® 594-conjugated Antibody

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Rat IgG1 Alexa Fluor® 700-conjugated Isotype Control

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Rat IgG1 Alexa Fluor® 405-conjugated Antibody

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Rat IgG1 Alexa Fluor® 750-conjugated Antibody

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Rat IgG1 Isotype Control

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