Rat IgG1 Isotype Control

Catalog # Availability Size / Price Qty
MAB005
IgG1 Isotype Control Control
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Product Details
Citations (43)
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Rat IgG1 Isotype Control Summary

Specificity
Selected for use as rat IgG1 isotype control. In direct ELISAs, no cross-react with recombinant human (rh) 6Ckine, recombinant mouse (rm) 6Ckine, rhACT II (rhMIP-1 beta ), rhBLC/BCA-1, rmC10, recombinant rat (rr) CINC-1, rrCINC-2 alpha, rrCINC-2 beta, rmCRG-2, rhENA-78, rhEotaxin, rmEotaxin, rhEotaxin 2, rhFractalkine, rmFractalkine, rhGCP-2, rmGCP-2, rhGRO alpha, rhGRO beta, rhGRO gamma, rhHCC-1, rhHCC-4, rhI-309, rhIL-8, rhIL-16, rhIP-10, rmJE, rmKC, rmLymphotactin, rmMARC, rhMCP-1, rhMCP-1 R, rhMCP-2, rhMCP-3, rhMCP-4, rmMCP-5, rhMDC, rmMDC, rhMIG, rmMIG, rhMIP-1 alpha, rmMIP-1 alpha, rmMIP-1 beta, rmMIP-1 gamma, rhMIP-1δ, rmMIP-2, rhMIP-3 alpha, rrMIP-3 alpha, rhMIP-3 beta, rmMIP-3 beta, recombinant viral (rv) MIP-I, rvMIP-II, rvMIP-III, rhMPIF-1, rhNAP-2, rhParc, rhRANTES, rmRANTES, rhSDF-1 alpha, rmSDF-1 alpha, rhSDF-1 beta, rhTarc, rmTCA-3, rhTeck, or rmTeck.
Source
Monoclonal Rat IgG1 Clone # 43414
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
KLH
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Control
Negative control for use in conjunction with R&D Systems antibodies. Use at the same concentration as the detection antibody.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Detection of Rat IgGControl by Flow Cytometry Mouse platelets were stained with Rat Anti-Mouse GPVIMonoclonal Antibody (Catalog # MAB6758,filled histogram) or Rat IgG Isotype Control Antibody (Catalog # MAB005,open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG SecondaryAntibody (Catalog # F0113).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IgG1

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name
Immunoglobulin G1
Alternate Names
IgG1

Product Datasheets

Citations for Rat IgG1 Isotype Control

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

43 Citations: Showing 1 - 10
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  1. Regulation of the bi-directional cross-talk between ovarian cancer cells and adipocytes by SPARC
    Authors: B John, C Naczki, C Patel, A Ghoneum, S Qasem, Z Salih, N Said
    Oncogene, 2019;0(0):.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Isotype Control
  2. Modulating bone marrow hematopoietic lineage potential to prevent bone metastasis in breast cancer
    Authors: JM Ubellacker, N Baryawno, N Severe, MJ DeCristo, J Sceneay, JN Hutchinson, MT Haider, CS Rhee, Y Qin, WM Gregory, AC Garrido-Ca, I Holen, JE Brown, RE Coleman, DT Scadden, SS McAllister
    Cancer Res., 2018;0(0):.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  3. Estrogen Deficiency Promotes Cerebral Aneurysm Rupture by Upregulation of Th17 Cells and Interleukin-17A Which Downregulates E-Cadherin
    Authors: BL Hoh, K Rojas, L Lin, HZ Fazal, S Hourani, KW Nowicki, MB Schneider, K Hosaka
    J Am Heart Assoc, 2018;7(8):.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  4. Induction of necroptotic cell death by viral activation of the RIG-I or STING pathway
    Authors: SN Schock, NV Chandra, Y Sun, T Irie, Y Kitagawa, B Gotoh, L Coscoy, A Winoto
    Cell Death Differ, 2017;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  5. Blockade of interleukin-6 receptor in the periphery promotes rapid and sustained antidepressant actions: a possible role of gut-microbiota-brain axis
    Authors: JC Zhang, W Yao, C Dong, C Yang, Q Ren, M Ma, K Hashimoto
    Transl Psychiatry, 2017;7(5):e1138.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Isotype Control
  6. Selenoprotein P-neutralizing antibodies improve insulin secretion and glucose sensitivity in type 2 diabetes mouse models
    Authors: Y Mita, K Nakayama, S Inari, Y Nishito, Y Yoshioka, N Sakai, K Sotani, T Nagamura, Y Kuzuhara, K Inagaki, M Iwasaki, H Misu, M Ikegawa, T Takamura, N Noguchi, Y Saito
    Nat Commun, 2017;8(1):1658.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In vivo Control
  7. Cooperation of Gastric Mononuclear Phagocytes with Helicobacter pylori during Colonization
    Authors: M Viladomiu, J Bassaganya, N Tubau-Juni, B Kronsteine, A Leber, CW Philipson, V Zoccoli-Ro, R Hontecilla
    J. Immunol, 2017;0(0):.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  8. Granulocyte colony-stimulating factor blockade enables dexamethasone to inhibit lipopolysaccharide-induced murine lung neutrophils
    Authors: J Banuelos, Y Cao, SC Shin, BS Bochner, P Avila, S Li, X Jiang, MW Lingen, RP Schleimer, NZ Lu
    PLoS ONE, 2017;12(5):e0177884.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  9. Autoimmune Renal Disease Is Exacerbated by S1P-Receptor-1-Dependent Intestinal Th17 Cell Migration to the Kidney
    Authors: Ulf Panzer
    Immunity, 2016;45(5):1078-1092.
    Species: Mouse
    Sample Types:
    Applications: Neutralization
  10. Presentation of Cryptic Peptides by MHC Class I Is Enhanced by Inflammatory Stimuli
    J Immunol, 2016;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  11. Role of G-CSF in monophosphoryl lipid A-mediated augmentation of neutrophil functions after burn injury.
    Authors: Bohannon J, Luan L, Hernandez A, Afzal A, Guo Y, Patil N, Fensterheim B, Sherwood E
    J Leukoc Biol, 2016;99(4):629-40.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  12. IL6 Blockade Reprograms the Lung Tumor Microenvironment to Limit the Development and Progression of K-ras-Mutant Lung Cancer
    Authors: MS Caetano, H Zhang, AM Cumpian, L Gong, N Unver, EJ Ostrin, S Daliri, SH Chang, CE Ochoa, S Hanash, C Behrens, II Wistuba, C Sternberg, H Kadara, CG Ferreira, SS Watowich, SJ Moghaddam
    Cancer Res., 2016;76(11):3189-99.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  13. Cessation of CCL2 inhibition accelerates breast cancer metastasis by promoting angiogenesis.
    Authors: Bonapace L, Coissieux M, Wyckoff J, Mertz K, Varga Z, Junt T, Bentires-Alj M
    Nature, 2015;515(7525):130-3.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  14. G-CSF Promotes Neuroblastoma Tumorigenicity and Metastasis via STAT3-Dependent Cancer Stem Cell Activation.
    Authors: Agarwal S, Lakoma A, Chen Z, Hicks J, Metelitsa L, Kim E, Shohet J
    Cancer Res, 2015;75(12):2566-79.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  15. Mechanisms of Hypoxic Up-Regulation of Versican Gene Expression in Macrophages.
    Authors: Sotoodehnejadnematalahi F, Staples K, Chrysanthou E, Pearson H, Ziegler-Heitbrock L, Burke B
    PLoS ONE, 2015;10(6):e0125799.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  16. Neuropathic pain is constitutively suppressed in early life by anti-inflammatory neuroimmune regulation.
    Authors: McKelvey R, Berta T, Old E, Ji R, Fitzgerald M
    J Neurosci, 2015;35(2):457-66.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  17. Apolipoprotein E promotes subretinal mononuclear phagocyte survival and chronic inflammation in age-related macular degeneration.
    Authors: Levy O, Calippe B, Lavalette S, Hu S, Raoul W, Dominguez E, Housset M, Paques M, Sahel J, Bemelmans A, Combadiere C, Guillonneau X, Sennlaub F
    EMBO Mol Med, 2015;7(2):211-26.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  18. Coordinate expansion of murine hematopoietic and mesenchymal stem cell compartments by SHIPi.
    Authors: Brooks R, Iyer S, Akada H, Neelam S, Russo C, Chisholm J, Kerr W
    Stem Cells, 2015;33(3):848-58.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization Control
  19. Critical role for IL-18 in spontaneous lung inflammation caused by autophagy deficiency.
    Authors: Abdel Fattah E, Bhattacharya A, Herron A, Safdar Z, Eissa N
    J Immunol, 2015;194(11):5407-16.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization Control
  20. Coexistent ARID1A-PIK3CA mutations promote ovarian clear-cell tumorigenesis through pro-tumorigenic inflammatory cytokine signalling.
    Authors: Chandler, Ronald L, Damrauer, Jeffrey, Raab, Jesse R, Schisler, Jonathan, Wilkerson, Matthew, Didion, John P, Starmer, Joshua, Serber, Daniel, Yee, Della, Xiong, Jessie, Darr, David B, Pardo-Manuel de Villena, Fernando, Kim, William, Magnuson, Terry
    Nat Commun, 2015;6(0):6118.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization Control
  21. Targeting DNGR-1 (CLEC9A) with antibody/MUC1 peptide conjugates as a vaccine for carcinomas.
    Authors: Picco G, Beatson R, Taylor-Papadimitriou J, Burchell J
    Eur J Immunol, 2014;44(7):1947-55.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  22. Syndecan-1 in the mouse parietal peritoneum microcirculation in inflammation.
    Authors: Kowalewska P, Patrick A, Fox-Robichaud A
    PLoS ONE, 2014;9(9):e104537.
    Species: Mouse
    Sample Types: In Vivo
    Applications: IHC-Fr
  23. Catecholamine stress alters neutrophil trafficking and impairs wound healing by beta2-adrenergic receptor-mediated upregulation of IL-6.
    Authors: Kim M, Gorouhi F, Ramirez S, Granick J, Byrne B, Soulika A, Simon S, Isseroff R
    J Invest Dermatol, 2014;134(3):809-17.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  24. Retinoic acid prevents mesenteric lymph node dendritic cells from inducing IL-13-producing inflammatory Th2 cells.
    Authors: Yokota-Nakatsuma A, Takeuchi H, Ohoka Y, Kato C, Song S, Hoshino T, Yagita H, Ohteki T, Iwata M
    Mucosal Immunol, 2014;7(4):786-801.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Isotype Control
  25. Leukocyte attraction by CCL20 and its receptor CCR6 in humans and mice with pneumococcal meningitis.
    Authors: Klein M, Brouwer M, Angele B, Geldhoff M, Marquez G, Varona R, Hacker G, Schmetzer H, Hacker H, Hammerschmidt S, van der Ende A, Pfister H, van de Beek D, Koedel U
    PLoS ONE, 2014;9(4):e93057.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  26. The role of host and microbial factors in the pathogenesis of pneumococcal bacteraemia arising from a single bacterial cell bottleneck.
    Authors: Gerlini A, Colomba L, Furi L, Braccini T, Manso A, Pammolli A, Wang B, Vivi A, Tassini M, Van Rooijen N, Pozzi G, Ricci S, Andrew P, Koedel U, Moxon E, Oggioni M
    PLoS Pathog, 2014;10(3):e1004026.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  27. Netrin-1 promotes adipose tissue macrophage retention and insulin resistance in obesity.
    Authors: Ramkhelawon B, Hennessy E, Menager M, Ray T, Sheedy F, Hutchison S, Wanschel A, Oldebeken S, Geoffrion M, Spiro W, Miller G, McPherson R, Rayner K, Moore K
    Nat Med, 2014;20(4):377-84.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  28. G-CSF drives a posttraumatic immune program that protects the host from infection.
    Authors: Gardner J, Noel J, Nikolaidis N, Karns R, Aronow B, Ogle C, McCormack F
    J Immunol, 2014;192(5):2405-17.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  29. CCR6 is required for epidermal trafficking of gammadelta-T cells in an IL-23-induced model of psoriasiform dermatitis.
    Authors: Mabuchi T, Singh T, Takekoshi T, Jia G, Wu X, Kao M, Weiss I, Farber J, Hwang S
    J Invest Dermatol, 2013;133(1):164-71.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization Control
  30. Acute lung injury and acute kidney injury are established by four hours in experimental sepsis and are improved with pre, but not post, sepsis administration of TNF-alpha antibodies.
    Authors: Bhargava R, Altmann C, Andres-Hernando A, Webb R, Okamura K, Yang Y, Falk S, Schmidt E, Faubel S
    PLoS ONE, 2013;8(11):e79037.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Isotype Control
  31. Canonical and noncanonical Hedgehog pathway in the pathogenesis of multiple myeloma.
    Authors: Blotta, Simona, Jakubikova, Jana, Calimeri, Teresa, Roccaro, Aldo M, Amodio, Nicola, Azab, Abdel Ka, Foresta, Umberto, Mitsiades, Constant, Rossi, Marco, Todoerti, Katia, Molica, Stefano, Morabito, Fortunat, Neri, Antonino, Tagliaferri, Piersand, Tassone, Pierfran, Anderson, Kenneth, Munshi, Nikhil C
    Blood, 2012;120(25):5002-13.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry Control
  32. GDF-15 is an inhibitor of leukocyte integrin activation required for survival after myocardial infarction in mice.
    Authors: Kempf T, Zarbock A, Widera C, Butz S, Stadtmann A, Rossaint J, Bolomini-Vittori M, Korf-Klingebiel M, Napp LC, Hansen B, Kanwischer A, Bavendiek U, Beutel G, Hapke M, Sauer MG, Laudanna C, Hogg N, Vestweber D, Wollert KC
    Nat. Med., 2011;17(5):581-8.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Control
  33. Tumor-derived G-CSF facilitates neoplastic growth through a granulocytic myeloid-derived suppressor cell-dependent mechanism.
    Authors: Waight JD, Hu Q, Miller A, Liu S, Abrams SI
    PLoS ONE, 2011;6(11):e27690.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Control
  34. Homeostatic imbalance of regulatory and effector T cells due to IL-2 deprivation amplifies murine lupus.
    Authors: Humrich JY, Morbach H, Undeutsch R, Enghard P, Rosenberger S, Weigert O, Kloke L, Heimann J, Gaber T, Brandenburg S, Scheffold A, Huehn J, Radbruch A, Burmester GR, Riemekasten G
    Proc. Natl. Acad. Sci. U.S.A., 2010;107(1):204-9.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Control
  35. G-CSF-initiated myeloid cell mobilization and angiogenesis mediate tumor refractoriness to anti-VEGF therapy in mouse models.
    Authors: Shojaei F, Wu X, Qu X, Kowanetz M, Yu L, Tan M, Meng YG, Ferrara N
    Proc. Natl. Acad. Sci. U.S.A., 2009;106(16):6742-7.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Control
  36. Tr1 regulatory T cells induced by ConA pretreatment prevent mice from ConA-induced hepatitis.
    Authors: Ye F, Yan S, Xu L, Jiang Z, Liu N, Xiong S, Wang Y, Chu Y
    Immunol. Lett., 2009;122(2):198-207.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Control
  37. Rapid dendritic cell mobilization to the large intestinal epithelium is associated with resistance to Trichuris muris infection.
    Authors: Cruickshank SM, Deschoolmeester ML, Svensson M, Howell G, Bazakou A, Logunova L, Little MC, English N, Mack M, Grencis RK, Else KJ, Carding SR
    J. Immunol., 2009;182(5):3055-62.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Control
  38. Anti-type II collagen antibody accelerates arthritis via CXCR2-expressing cells in IL-1 receptor antagonist-deficient mice.
    Authors: Kagari T, Tanaka D, Doi H, Iwakura Y, Shimozato T
    Eur. J. Immunol., 2007;37(10):2753-63.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Control
  39. IL-23 is required for neutrophil homeostasis in normal and neutrophilic mice.
    Authors: Smith E, Zarbock A, Stark MA, Burcin TL, Bruce AC, Foley P, Ley K
    J. Immunol., 2007;179(12):8274-9.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Control
  40. Junctional adhesion molecules (JAM)-B and -C contribute to leukocyte extravasation to the skin and mediate cutaneous inflammation.
    Authors: Ludwig RJ, Zollner TM, Santoso S, Hardt K, Gille J, Baatz H, Johann PS, Pfeffer J, Radeke HH, Schon MP, Kaufmann R, Boehncke WH, Podda M
    J. Invest. Dermatol., 2005;125(5):969-76.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Control
  41. MIP-1 gamma promotes receptor-activator-of-NF-kappa-B-ligand-induced osteoclast formation and survival.
    Authors: Okamatsu Y, Battaglino R, Spate U, Stashenko P
    J. Immunol., 2004;173(3):2084-90.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Control
  42. MIP-1 gamma promotes receptor-activator-of-NF-kappa-B-ligand-induced osteoclast formation and survival.
    Authors: Okamatsu Y, Battaglino R, Spate U, Stashenko P
    J. Immunol., 2004;173(3):2084-90.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  43. Granulocyte colony-stimulating factor antibody abrogates radioprotective efficacy of gamma-tocotrienol, a promising radiation countermeasure.
    Authors: Kulkarni, Shilpa, Singh, Pankaj K, Ghosh, Sanchita, Posarac, Ana, Singh, Vijay K
    Cytokine, 213;62(2):278-85.
    Species: Mouse
    Sample Types: In Vivo

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