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Human LYAR Alexa Fluor® 405-conjugated Antibody

Catalog #: AF6748V Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF6748V-100UG

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Product Details
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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human LYAR Alexa Fluor® 405-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human LYAR in direct ELISAs and Western blots.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human LYAR
Lys288-Lys379
Accession # Q9NX58
Formulation
Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide
Label
Alexa Fluor 405 (Excitation= 405 nm, Emission= 421 nm)

Applications

Recommended Concentration
Sample
Western Blot
Optimal dilution of this antibody should be experimentally determined.
 
Immunocytochemistry
Optimal dilution of this antibody should be experimentally determined.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: LYAR

LYAR (Ly-1/CD5 antibody reactive clone) is a 45-50 kDa nucleolar protein that was named for the ability of its antibody to cross-react with Ly-1. Its function is unclear; it is known to associate with MYCN and RRP1B, the latter association giving rise to the suggestion that LYAR is involved with RNA metabolism. Human LYAR is 379 amino acids (aa) in length. It contains two C2H2-type Zn finger regions (aa 6-25 and 33-51) followed by one coiled-coil region (aa 175-219) and an NLS (aa 217-222). There are multiple Zn-binding sites and three utilized phosphorylation sites at Ser244, Ser258 and Ser276. Over aa 288-379, human LYAR shares 75% aa identity with mouse LYAR.

Long Name
Ly1 Antibody Reactive Homolog
Entrez Gene IDs
55646 (Human); 17089 (Mouse); 289707 (Rat)
Alternate Names
cell growth-regulating nucleolar protein; FLJ20425; Ly1 reactive homolog (mouse); LYAR; PNAS-5; ZLYAR

Product Datasheets

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