Human Melanocortin-5 R/MC5R PE-conjugated Antibody

FAB8205P has been discontinued. View all Melanocortin-5 R/MC5R products.
  
  • Species Reactivity
    Human
  • Specificity
    Stains human Melanocortin-5 R/MC5R transfected cells but not irrelevant transfectants in flow cytometry.
  • Source
    Monoclonal Mouse IgG2A Clone # 821012
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    NS0 mouse myeloma cell line transfected with human Melanocortin-5 R/MC5R
    Met1-Asp325
    Accession # P33032
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Phycoerythrin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Melanocortin-5 R/MC5R in Human Blood Monocytes by Flow Cytometry. Human peripheral blood monocytes were stained with Mouse Anti-Human Melanocortin-5 R/MC5R PE‑conjugated Monoclonal Antibody (Catalog # FAB8205P, filled histogram) or isotype control antibody (Catalog # IC003P, open histogram). View our protocol for Staining Membrane-associated Proteins.
Detection of Melanocortin-5 R/MC5R in HEK293 Human Cell Line Transfected with Human Melanocortin-5/MC5R and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with human Melanocortin-5/MC5R and eGFP (top) or irrelevant transfectant (bottom) was stained with Mouse Anti-Human Melanocortin-5 R/MC5R PE‑conjugated Monoclonal Antibody (Catalog # FAB8205P). Quadrant markers were set based on control antibody staining (Catalog # IC003P). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: Melanocortin-5 R/MC5R
Melanocortin-5 R (MC5R) is a member of the melanocortin receptor family. The 325 amino acid G-protein-coupled 7-transmembrane glycoprotein is expressed in skeletal muscle, where engagement by a-melanocyte stimulating hormone increases glucose uptake and stimulates a thermogenic response. Expression is also found in sebaceous glands and other skin structures, and on selected hematopoietic cell lines. Human MC5R shares 80-81% amino acid sequence identity with mouse and rat MC5R.
  • Long Name:
    Melanocortin 5 Receptor
  • Entrez Gene IDs:
    4161 (Human); 17203 (Mouse); 25726 (Rat)
  • Alternate Names:
    MC2; MC-2; MC5; MC5R; MC5-R; melanocortin 5 receptor; melanocortin receptor 5; Melanocortin-5 R; melanocortin-5 receptor; Melanocortin5R; Melanocortin-5R
Related Research Areas

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