Detection of Melanocortin-5 R/MC5R in Human Blood Monocytes by Flow Cytometry. Human peripheral blood monocytes were stained with Mouse Anti-Human Melanocortin-5 R/MC5R PE‑conjugated Monoclonal Antibody (Catalog # FAB8205P, filled histogram) or isotype control antibody (Catalog # IC003P, open histogram). View our protocol for Staining Membrane-associated Proteins.
Detection of Melanocortin-5 R/MC5R in HEK293 Human Cell Line Transfected with Human Melanocortin-5/MC5R and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with human Melanocortin-5/MC5R and eGFP (top) or irrelevant transfectant (bottom) was stained with Mouse Anti-Human Melanocortin-5 R/MC5R PE‑conjugated Monoclonal Antibody (Catalog # FAB8205P). Quadrant markers were set based on control antibody staining (Catalog # IC003P). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
Background: Melanocortin-5 R/MC5R
Melanocortin-5 R (MC5R) is a member of the melanocortin receptor family. The 325 amino acid G-protein-coupled 7-transmembrane glycoprotein is expressed in skeletal muscle, where engagement by a-melanocyte stimulating hormone increases glucose uptake and stimulates a thermogenic response. Expression is also found in sebaceous glands and other skin structures, and on selected hematopoietic cell lines. Human MC5R shares 80-81% amino acid sequence identity with mouse and rat MC5R.
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