Human Mesothelin Alexa Fluor® 594-conjugated Antibody

Catalog #: AF3265T Datasheet / COA / SDS

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AF3265T-100UG

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All Mesothelin Antibodies

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Human Mesothelin Alexa Fluor® 594-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human Mesothelin in direct ELISAs and Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Mesothelin isoform 2
Glu296-Gly580
Accession # ABW03459

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 594 (Excitation= 590 nm, Emission= 617 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: Mesothelin

Mesothelin is a 40 kDa, 292 amino acid (aa) GPI-linked glycoprotein found on normal mesothelial cells. It is also highly expressed in pancreatic, gastric and ovarian carcinomas. Mesothelin is synthesized as a preproprotein with a carboxy-terminal hydrophobic proregion that is removed when a GPI-anchor is added. The N-terminal prosegment is cleaved by furin to release a 33 kDa soluble protein that is also known as megakaryocyte potentiating factor (MPF). The membrane bound portion of Mesothelin can also be shed and is detectable in sera. Within the region used as the immunogen, human Mesothelin shares approximately 58% amino acid sequence identity with the mouse or rat protein.

Entrez Gene IDs

10232 (Human); 56047 (Mouse); 60333 (Rat); 102130460 (Cynomolgus Monkey)

Alternate Names

CAK1 antigen; CAK1; megakaryocyte potentiating factor; Mesothelin; MPF; MPFSMRP; MSLN; Pre-pro-megakaryocyte-potentiating factor; SMR; soluble MPF mesothelin related protein