Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Biotin

Antibody Source

Polyclonal Goat IgG
View all Mesothelin Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Mesothelin isoform 1
Glu296-Gly580
Accession # ABW03459

Specificity

Detects human Mesothelin in Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Human Mesothelin Biotinylated Antibody

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Human Mesothelin

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Mesothelin

Mesothelin is a 40 kDa, 292 amino acid (aa) GPI-linked glycoprotein found on normal mesothelial cells. It is also highly expressed in pancreatic, gastric and ovarian carcinomas. Mesothelin is synthesized as a preproprotein with a carboxy-terminal hydrophobic proregion that is removed when a GPI-anchor is added. The N-terminal prosegment is cleaved by furin to release a 33 kDa soluble protein that is also known as megakaryocyte potentiating factor (MPF). The membrane bound portion of Mesothelin can also be shed and is detectable in sera. Within the region used as the immunogen, human Mesothelin shares approximately 58% amino acid sequence identity with the mouse or rat protein.

Alternate Names

CAK1, MPF, MSLN, SMR

Entrez Gene IDs

10232 (Human); 56047 (Mouse); 60333 (Rat); 102130460 (Cynomolgus Monkey)

Gene Symbol

MSLN

UniProt

ABW03459

Additional Mesothelin Products

Product Documents for Human Mesothelin Biotinylated Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Mesothelin Biotinylated Antibody

For research use only

Related Research Areas

Cancer Biomarkers

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