Detection of Human Mesothelin by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Human Mesothelin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3265) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Mesothelin at approximately 69 kDa (as indicated). This experiment was conducted under reducing conditions and Immunoblot Buffer Group 8.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Mesothelin is a 40 kDa, 292 amino acid (aa) GPI-linked glycoprotein
found on normal mesothelial cells. It is also highly expressed in
pancreatic, gastric and ovarian carcinomas. Mesothelin is synthesized
as a preproprotein with a carboxy-terminal hydrophobic proregion that
is removed when a GPI-anchor is added. The N-terminal prosegment is
cleaved by furin to release a 33 kDa soluble protein that is also known
as megakaryocyte potentiating factor (MPF). The membrane bound portion
of Mesothelin can also be shed and is detectable in sera. Within the
region used as the immunogen, human Mesothelin shares approximately 58%
amino acid sequence identity with the mouse or rat protein.
Entrez Gene IDs:
10232 (Human); 56047 (Mouse); 60333 (Rat)
CAK1 antigen; CAK1; megakaryocyte potentiating factor; mesothelin; MPF; MPFSMRP; MSLN; Pre-pro-megakaryocyte-potentiating factor; SMR; soluble MPF mesothelin related protein
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