Human Mesothelin Antibody Summary
Accession # ABW03459
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human Mesothelin by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Human Mesothelin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3265) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Mesothelin at approximately 69 kDa (as indicated). This experiment was conducted under reducing conditions and Immunoblot Buffer Group 8.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Mesothelin is a 40 kDa, 292 amino acid (aa) GPI-linked glycoprotein found on normal mesothelial cells. It is also highly expressed in pancreatic, gastric and ovarian carcinomas. Mesothelin is synthesized as a preproprotein with a carboxy-terminal hydrophobic proregion that is removed when a GPI-anchor is added. The N-terminal prosegment is cleaved by furin to release a 33 kDa soluble protein that is also known as megakaryocyte potentiating factor (MPF). The membrane bound portion of Mesothelin can also be shed and is detectable in sera. Within the region used as the immunogen, human Mesothelin shares approximately 58% amino acid sequence identity with the mouse or rat protein.
No product specific FAQs exist for this product, however you mayView all Antibody FAQs
Reviews for Human Mesothelin Antibody
There are currently no reviews for this product. Be the first to review Human Mesothelin Antibody and earn rewards!
Have you used Human Mesothelin Antibody?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image