Human Mesothelin PerCP-conjugated Antibody

FAB32652C has been discontinued. View all Mesothelin products.
  
  • Species Reactivity
    Human
  • Specificity
    Detects human Mesothelin in direct ELISAs.
  • Source
    Monoclonal Rat IgG2A Clone # 420411
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human Mesothelin
    Glu296-Gly580
    Accession # AAH09272
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    PerCP (Peridinin-chlorophyll Protein Complex)
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Mesothelin in HeLa Human Cell Line by Flow Cytometry. HeLa human cervical epithelial carcinoma cell line was stained with Rat Anti-Human Mesothelin PerCP‑conjugated Monoclonal Antibody (Catalog # FAB32652C, filled histogram) or isotype control antibody (Catalog # IC006C, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: Mesothelin

Mesothelin is a 40 kDa glycosylphosphatidylinositol-anchored glycoprotein that is expressed on mesothelial cells in the pleura, pericardium and peritoneum and overexpressed in mesotheliomas and ovarian or pancreatic adenocarcinoma. Mesothelin is a product of the CAK-1 gene, which also encodes megakaryocyte-potentiating factor (MPF). Mature human mesothelin shares 60% amino acid identity with either mouse or rat mesothelin. Two variant forms exist; variant 1 has an eight amino acid (1 kDa) insertion and is rarely expressed, while variant 2 is a truncated form secreted in the majority of ovarian cancers but rarely found in normal individuals.

  • Entrez Gene IDs:
    10232 (Human); 56047 (Mouse); 60333 (Rat)
  • Alternate Names:
    CAK1 antigen; CAK1; megakaryocyte potentiating factor; Mesothelin; MPF; MPFSMRP; MSLN; Pre-pro-megakaryocyte-potentiating factor; SMR; soluble MPF mesothelin related protein
Related Research Areas

Have you used Human Mesothelin PerCP-conjugated Antibody?

Submit a review and receive a $25US/€18/£15/$25CAN amazon gift card if you include an image - $10US/€7/£6/$10CAN Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.

Order Details

Contact Information

Calculators

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷
R&D Systems Guarantee

Monoclonal
Antibody
Panels For
Screening

Learn More
Monoclonal Antibodies

Feedback

Customer Information