Human MICA Alexa Fluor® 405-conjugated Antibody

FAB1300V Datasheet / COA / SDS

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FAB1300V-100UG

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Human MICA Alexa Fluor® 405-conjugated Antibody in Flow Cytometry

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Human MICA Alexa Fluor® 405-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human MICA in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human MICB is observed.

Source

Monoclonal Mouse IgG_2B Clone # 159227

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

BaF3 mouse pro-B cell line transfected with human MICA

Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Label

Alexa Fluor 405 (Excitation= 405 nm, Emission= 421 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25-1 µg/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry

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Detection of MICA in K562 Human Cell Line by Flow Cytometry. K562 human chronic myelogenous leukemia cell line was stained with Mouse Anti-Human MICA Alexa Fluor® 405-conjugated Monoclonal Antibody (Catalog # FAB1300V, filled histogram) or isotype control antibody (Catalog # IC0041V, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: MICA

MICA (MHC class I chain-related gene A) is a transmembrane glycoprotein that functions as a ligand for human NKG2D. A closely related protein, MICB, shares 85% amino acid identity with MICA. These proteins are distantly related to the MHC class I proteins. They possess three extracellular Ig-like domains, but they have no capacity to bind peptide or interact with beta 2-microglobulin. The genes encoding these proteins are found within the Major Histocompatibility Complex on human chromosome 6. The MICA locus is highly polymorphic with more than 50 recognized human alleles. MICA is absent from most cells but is frequently expressed in epithelial tumors and can be induced by bacterial and viral infections. MICA is a ligand for human NKG2D, an activating receptor expressed on NK cells, NKT cells, gamma δ T cells, and CD8⁺ alpha beta T cells. Recognition of MICA by NKG2D results in the activation of cytolytic activity and/or cytokine production by these effector cells. MICA recognition is involved in tumor surveillance, viral infections, and autoimmune diseases.

References