Human MICA Alexa Fluor® 488-conjugated Antibody

  • Species Reactivity
  • Specificity
    Detects human MICA in direct ELISAs and Western blots. In direct ELISAs, this antibody does not cross-react with recombinant human MICB.
  • Source
    Monoclonal Mouse IgG2B Clone # 159227
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    BaF3 mouse pro-B cell line transfected with human MICA
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Alexa Fluor 488
  • Flow Cytometry
    5 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of MICA in K562 Human Cell Line by Flow Cytometry. K562 human chronic myelogenous leukemia cell line was stained with Mouse Anti-Human MICA Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # FAB1300G, filled histogram) or isotype control antibody (Catalog # IC0041G, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: MICA

MICA (MHC class I chain-related gene A) is a transmembrane glycoprotein that functions as a ligand for human NKG2D. A closely related protein, MICB, shares 85% amino acid identity with MICA. These proteins are distantly related to the MHC class I proteins. They possess three extracellular Ig-like domains, but they have no capacity to bind peptide or interact with beta 2-microglobulin. The genes encoding these proteins are found within the Major Histocompatibility Complex on human chromosome 6. The MICA locus is highly polymorphic with more than 50 recognized human alleles. MICA is absent from most cells but is frequently expressed in epithelial tumors and can be induced by bacterial and viral infections. MICA is a ligand for human NKG2D, an activating receptor expressed on NK cells, NKT cells, gamma δ T cells, and CD8+ alpha beta T cells. Recognition of MICA by NKG2D results in the activation of cytolytic activity and/or cytokine production by these effector cells. MICA recognition is involved in tumor surveillance, viral infections, and autoimmune diseases.

  • References:
    1. Groh, V. et al. (2001) Nature Immunol. 2:255.
    2. Stephens, H. (2001) Trends Immunol. 22:378.
    3. Bauer, S. et al. (1999) Science 285:727.
    4. Groh, V. et al. (2002) Nature 419:734.
    5. Steinle, A. et al. (2001) Immunogenetics 53:279.
    6. Pende, D. et al. (2002) Cancer Res. 62:6178.
    7. NKG2D and its Ligands (2002)
  • Long Name:
    MHC Class I-related Protein A
  • Entrez Gene IDs:
    100507436 (Human)
  • Alternate Names:
    FLJ60820; MGC111087; PERB11.1
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG2B Alexa Fluor® 488-conjugated Isotype Control

Ctrl IC0041G  
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Staining Reagents
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Flow Cytometry Staining Buffer (1X)

Flow FC001 3
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 1
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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