Human MICA PE-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB1300P-025
FAB1300P-100
Human MICA PE-conjugated Antibody in Flow Cytometry
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Product Details
Citations (9)
FAQs
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Human MICA PE-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human MICA in direct ELISAs and Western blots. In direct ELISAs, this antibody does not cross-react with recombinant human MICB.
Source
Monoclonal Mouse IgG2B Clone # 159227
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
BaF3 mouse pro-B cell line transfected with human MICA
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below
Knockout Validated
MICA is specifically detected in K562 myelogenous leukemia parental cell line but is not detectable in MICA knockout K562 cell line.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Flow Cytometry Detection of MICA in K562 Human Cell Line by Flow Cytometry. View Larger

Detection of MICA in K562 Human Cell Line by Flow Cytometry. K562 human chronic myelogenous leukemia cell line was stained with Mouse Anti-Human MICA PE-conjugated Monoclonal Antibody (Catalog # FAB1300P, filled histogram) or isotype control antibody (Catalog # IC0041P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Knockout Validated MICA Specificity is Shown by Flow Cytometry in Knockout Cell Line. View Larger

MICA Specificity is Shown by Flow Cytometry in Knockout Cell Line. MICA knockout K562 human myelogenous leukemia cell line was stained with Mouse Anti-Human MICA PE-conjugated Monoclonal Antibody (Catalog # FAB1300P, filled histogram) or isotype control antibody (Catalog # IC0041P, open histogram). No staining in the MICA knockout K562 cell line was observed. View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: MICA

MICA (MHC class I chain-related gene A) is a transmembrane glycoprotein that functions as a ligand for human NKG2D. A closely related protein, MICB, shares 85% amino acid identity with MICA. These proteins are distantly related to the MHC class I proteins. They possess three extracellular Ig-like domains, but they have no capacity to bind peptide or interact with beta 2-microglobulin. The genes encoding these proteins are found within the Major Histocompatibility Complex on human chromosome 6. The MICA locus is highly polymorphic with more than 50 recognized human alleles. MICA is absent from most cells but is frequently expressed in epithelial tumors and can be induced by bacterial and viral infections. MICA is a ligand for human NKG2D, an activating receptor expressed on NK cells, NKT cells, gamma δ T cells, and CD8+ alpha beta T cells. Recognition of MICA by NKG2D results in the activation of cytolytic activity and/or cytokine production by these effector cells. MICA recognition is involved in tumor surveillance, viral infections, and autoimmune diseases.

References
  1. Groh, V. et al. (2001) Nature Immunol. 2:255.
  2. Stephens, H. (2001) Trends Immunol. 22:378.
  3. Bauer, S. et al. (1999) Science 285:727.
  4. Groh, V. et al. (2002) Nature 419:734.
  5. Steinle, A. et al. (2001) Immunogenetics 53:279.
  6. Pende, D. et al. (2002) Cancer Res. 62:6178.
  7. NKG2D and its Ligands (2002) http://www.RnDSystems.com
Long Name
MHC Class I-related Protein A
Entrez Gene IDs
100507436 (Human)
Alternate Names
FLJ60820; MGC111087; MICA; PERB11.1

Product Datasheets

Citations for Human MICA PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

9 Citations: Showing 1 - 9
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  1. Impaired NK cell recognition of vemurafenib-treated melanoma cells is overcome by simultaneous application of histone deacetylase inhibitors
    Authors: S López-Cobo, N Pieper, C Campos-Sil, EM García-Cue, HT Reyburn, A Paschen, M Valés-Góme
    Oncoimmunology, 2018;7(2):e1392426.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Low-dose bortezomib increases the expression of NKG2D and DNAM-1 ligands and enhances induced NK and ?? T cell-mediated lysis in multiple myeloma
    Authors: C Niu, H Jin, M Li, S Zhu, L Zhou, F Jin, Y Zhou, D Xu, J Xu, L Zhao, S Hao, W Li, J Cui
    Oncotarget, 2017;8(4):5954-5964.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. All-trans retinoic acid enhances cytotoxicity of CIK cells against human lung adenocarcinoma by upregulating MICA and IL-2 secretion
    Authors: XY Fan, PY Wang, C Zhang, YL Zhang, Y Fu, C Zhang, QX Li, JN Zhou, BE Shan, DW He
    Sci Rep, 2017;7(1):16481.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. SEP enhanced the antitumor activity of 5-fluorouracil by up-regulating NKG2D/MICA and reversed immune suppression via inhibiting ROS and caspase-3 in mice
    Authors: M Ke, H Wang, Y Zhou, J Li, Y Liu, M Zhang, J Dou, T Xi, B Shen, C Zhou
    Oncotarget, 2016;7(31):49509-49526.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. A polysaccharide virulence factor of a human fungal pathogen induces neutrophil apoptosis via NK cells.
    Authors: Robinet P, Baychelier F, Fontaine T, Picard C, Debre P, Vieillard V, Latge J, Elbim C
    J Immunol, 2014;192(11):5332-42.
    Species: Human
    Sample Types: Whole Blood
    Applications: Flow Cytometry
  6. Increase of IFN-gamma and TNF-alpha production in CD107a + NK-92 cells co-cultured with cervical cancer cell lines pre-treated with the HO-1 inhibitor.
    Authors: Gomez-Lomeli P, Bravo-Cuellar A, Hernandez-Flores G, Jave-Suarez L, Aguilar-Lemarroy A, Lerma-Diaz J, Dominguez-Rodriguez J, Sanchez-Reyes K, Ortiz-Lazareno P
    Cancer Cell Int, 2014;14(1):100.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  7. HER2/HER3 signaling regulates NK cell-mediated cytotoxicity via MHC class I chain-related molecule A and B expression in human breast cancer cell lines.
    Authors: Okita R, Mougiakakos D, Ando T, Mao Y, Sarhan D, Wennerberg E, Seliger B, Lundqvist A, Mimura K, Kiessling R
    J. Immunol., 2012;188(5):2136-45.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  8. The MHC class Ib protein ULBP1 is a nonredundant determinant of leukemia/lymphoma susceptibility to gammadelta T-cell cytotoxicity.
    Authors: Lanca T, Correia DV, Moita CF, Raquel H, Neves-Costa A, Ferreira C, Ramalho JS, Barata JT, Moita LF, Gomes AQ, Silva-Santos B
    Blood, 2010;115(12):2407-11.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  9. Expression of WSX1 in tumors sensitizes IL-27 signaling-independent natural killer cell surveillance.
    Authors: Dibra D, Cutrera JJ, Xia X, Birkenbach MP, Li S
    Cancer Res., 2009;69(13):5505-13.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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