Intracellular Staining by Flow Cytometry
|Detection of MINA in Jurkat Human Cell Line by Flow Cytometry. Jurkat human acute T cell leukemia cell line was stained with Mouse Anti-Human MINA Allophycocyanin‑conjugated Monoclonal Antibody (Catalog # IC7476A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.|
MINA (Myc-induced Nuclear Antigen), also known as Mina53, is a 52-54 kDa member of both the MINA53/NO66 and Jumonji C family of proteins. It expression is associated with proliferating cells, and it has been found in cytoplasm, nucleus and particularly nucleoli. MINA appears to be induced by c-myc, and synthesized by spermatogonia, select epithelium, naïve T cells and select cancer cells. When expressed, MINA is reported to regulate expression of genes such as HGF, EGF-R, IL-4, and Relm beta, the latter through the influence of TGF-beta. It may exert its regulatory activity through an intrinsic histone demethylase function. Human MINA is 465 amino acids (aa) in length. It possesses one cupin (or enzyme‑associated) region (aa 52-364) that contains a JmjC domain (aa 139-271). There are three potential isoform variants that show either a seven aa substitution for aa 255‑261, an 18 aa substitution for aa 263-465, or a deletion of Glu297. Over aa 2-192, human MINA shares 82% aa sequence identity with mouse MINA.