Human/Mouse 4EBP1 Alexa Fluor® 594-conjugated Antibody

Catalog #: AF3227T Datasheet / COA / SDS

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AF3227T-100UG

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All 4EBP1 Antibodies

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Human/Mouse 4EBP1 Alexa Fluor® 594-conjugated Antibody Summary

Species Reactivity

Human, Mouse

Specificity

Detects human and mouse 4EBP1.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human 4EBP1
Ser2-Ile118
Accession # Q13541

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 594 (Excitation= 590 nm, Emission= 617 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Knockout Validated

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: 4EBP1

The eukaryotic initiation factor 4E (eIF-4E) binding protein 1 (4EBP1) binds to eIF-4E to prevent translation initiation. Upon phosphorylation by the target of rapamycin (TOR) kinase, 4EBP1 releases eIF-4E and translation initiation ensues. 4EBP1 is also known as PHAS-1 (phosphorylated heat and acid stable protein regulated by insulin 1).

Long Name

Eukaryotic Translation Initiation Factor 4E Binding Protein 1

Entrez Gene IDs

1978 (Human); 13685 (Mouse); 116636 (Rat)

Alternate Names

4EBP1; 4E-BP1; eIF4E-binding protein 1; EIF4EBP1; eukaryotic translation initiation factor 4E binding protein 1,4EBP1,4E-BP1BP-1; eukaryotic translation initiation factor 4E-binding protein 1; PHAS-I; PHAS-IMGC4316; Phosphorylated heat- and acid-stable protein regulated by insulin 1