Human/Mouse DCBLD2/ESDN PE-conjugated Antibody

Applications

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Preparation and Storage

Background: DCBLD2/ESDN

DCBLD2 (Discoidin, CUB and CLLC Domain-containing 2), also known as ESDN and CLCP1, is a novel, 95-130 kDa protein that bears strong resemblance to the neuropilin family of proteins. It is a type I transmembrane glycoprotein whose ORF contains an extremely long signal sequence of 66 amino acid (aa) length. Mature human DCBLD2 is 709 aa in length, and contains a 462 aa extracellular region that is characterized by the presence of one CUB and F5/8 (or discoidin) domain (and thus its resemblance to Neuropilin), plus one 93 aa LCCL domain. Over aa 67-528, human and mouse DCBLD2 share 87% aa sequence identity. Although DCBLD2 expression is widespread (if not ubiquitous), cell types specifically reported to contain DCBLD2 include skeletal muscle cells, vascular endothelial and smooth muscle cells, and various primary tumor cells. The large variation in native molecular weight is attributable to a variable glycosylation state. It would appear that the higher MW forms are more sensitive to ligation than the lower MW forms, and one possible ligand for DCBLD2 has been suggested to be SEMA4B. The exact nature of the DCBLD2 signaling complex is unclear; it has been proposed that multiple interacting partners exist in-cis, and may include InsRb, VEGFR2, and PEGFR beta. Notably, DCBLD2 appears to regulate the ubiquitination state of its receptor partners, and may either upregulate or downregulate receptor activity in a cell-specific manner.

Product Datasheets

Related Research Areas

• Adhesive Interactions in Allergy and Asthma
• Cancer Biomarkers
• Endothelial Cell Markers
• Semaphorins, Plexin Receptors, and Related Molecules