Human/Mouse DCBLD2/ESDN PE-conjugated Antibody

FAB6269P has been discontinued. View all DCBLD2/ESDN products.
  
  • Species Reactivity
    Human, Mouse
  • Specificity
    Detects human and mouse DCBLD2/ESDN in Western blots and human DCBLD2/ESDN in direct ELISAs.
  • Source
    Polyclonal Sheep IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human DCBLD2/ESDN
    Gln67-Ala528
    Accession # Q96PD2
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Phycoerythrin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of DCBLD2/ESDN in NCI‑H460 Human Cell Line and bEnd.3 Mouse Cell Line by Flow Cytometry. NCI‑H460 human large cell lung carcinoma cell line (panel A) and bEnd.3 mouse endothelioma cell line (panel B) were stained with Sheep Anti-Human/Mouse DCBLD2/ESDN PE‑conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB6269P, filled histogram) or isotype control antibody (Catalog # IC016P, open histogram). View our protocol for Staining Membrane-associated Proteins
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: DCBLD2/ESDN
DCBLD2 (Discoidin, CUB and CLLC Domain-containing 2), also known as ESDN and CLCP1, is a novel, 95-130 kDa protein that bears strong resemblance to the neuropilin family of proteins. It is a type I transmembrane glycoprotein whose ORF contains an extremely long signal sequence of 66 amino acid (aa) length. Mature human DCBLD2 is 709 aa in length, and contains a 462 aa extracellular region that is characterized by the presence of one CUB and F5/8 (or discoidin) domain (and thus its resemblance to Neuropilin), plus one 93 aa LCCL domain. Over aa 67-528, human and mouse DCBLD2 share 87% aa sequence identity. Although DCBLD2 expression is widespread (if not ubiquitous), cell types specifically reported to contain DCBLD2 include skeletal muscle cells, vascular endothelial and smooth muscle cells, and various primary tumor cells. The large variation in native molecular weight is attributable to a variable glycosylation state. It would appear that the higher MW forms are more sensitive to ligation than the lower MW forms, and one possible ligand for DCBLD2 has been suggested to be SEMA4B. The exact nature of the DCBLD2 signaling complex is unclear; it has been proposed that multiple interacting partners exist in-cis, and may include InsRb, VEGFR2, and PEGFR beta. Notably, DCBLD2 appears to regulate the ubiquitination state of its receptor partners, and may either upregulate or downregulate receptor activity in a cell-specific manner.
  • Long Name:
    Discoidin, CUB and LCCL Domain Containing 2/Endothelial and Smooth Muscle Cell-derived Neuropilin-li
  • Entrez Gene IDs:
    131566 (Human); 73379 (Mouse); 155696 (Rat)
  • Alternate Names:
    CLCP1; CLCP11700055P21Rik; CUB, LCCL and coagulation factor V/VIII-homology domains protein 1; DCBLD2; discoidin, CUB and LCCL domain containing 2; discoidin, CUB and LCCL domain-containing protein 2; Endothelial and smooth muscle cell-derived neuropilin-like protein; ESDN; ESDNcoagulation factor V/VIII-homology domains protein 1
Related Research Areas

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