Detection of DCBLD2/ESDN in NCI‑H460 Human Cell Line and bEnd.3 Mouse Cell Line by Flow Cytometry. NCI‑H460 human large cell lung carcinoma cell line (panel A) and bEnd.3 mouse endothelioma cell line (panel B) were stained with Sheep Anti-Human/Mouse DCBLD2/ESDN PE‑conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB6269P, filled histogram) or isotype control antibody (Catalog # IC016P, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
DCBLD2 (Discoidin, CUB and CLLC Domain-containing
2), also known as ESDN and CLCP1, is a novel, 95-130 kDa protein that bears strong
resemblance to the neuropilin family of proteins. It is a type I transmembrane glycoprotein
whose ORF contains an extremely long signal sequence of 66 amino acid (aa)
length. Mature human DCBLD2 is 709 aa in
length, and contains a 462 aa extracellular region that is characterized by the
presence of one CUB and F5/8 (or discoidin) domain (and thus its resemblance to
Neuropilin), plus one 93 aa LCCL domain. Over aa 67-528, human and mouse DCBLD2
share 87% aa sequence identity. Although
DCBLD2 expression is widespread (if not ubiquitous), cell types specifically
reported to contain DCBLD2 include skeletal muscle cells, vascular endothelial
and smooth muscle cells, and various primary tumor cells. The large variation in native molecular
weight is attributable to a variable glycosylation state. It would appear that the higher MW forms are
more sensitive to ligation than the lower MW forms, and one possible ligand for
DCBLD2 has been suggested to be SEMA4B.
The exact nature of the DCBLD2 signaling complex is unclear; it has been
proposed that multiple interacting partners exist in-cis, and may include InsRb, VEGFR2, and PEGFR beta. Notably, DCBLD2 appears to regulate the
ubiquitination state of its receptor partners, and may either upregulate or
downregulate receptor activity in a cell-specific manner.
Discoidin, CUB and LCCL Domain Containing 2/Endothelial and Smooth Muscle Cell-derived Neuropilin-li
Entrez Gene IDs:
131566 (Human); 73379 (Mouse); 155696 (Rat)
CLCP1; CLCP11700055P21Rik; CUB, LCCL and coagulation factor V/VIII-homology domains protein 1; discoidin, CUB and LCCL domain containing 2; discoidin, CUB and LCCL domain-containing protein 2; Endothelial and smooth muscle cell-derived neuropilin-like protein; ESDN; ESDNcoagulation factor V/VIII-homology domains protein 1
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