Human/Mouse DCBLD2/ESDN PE‑conjugated Antibody

R&D Systems | Catalog # FAB6269P

R&D Systems
Discontinued Product
FAB6269P has been discontinued. View all DCBLD2/ESDN products.

Key Product Details

Species Reactivity

Human, Mouse

Applications

Flow Cytometry

Label

Phycoerythrin (Excitation = 488 nm, Emission = 565-605 nm)

Antibody Source

Polyclonal Sheep IgG
View all DCBLD2/ESDN Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human DCBLD2/ESDN
Gln67-Ala528
Accession # Q96PD2

Specificity

Detects human and mouse DCBLD2/ESDN in Western blots and human DCBLD2/ESDN in direct ELISAs.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human/Mouse DCBLD2/ESDN PE‑conjugated Antibody

Detection of DCBLD2/ESDN antibody in NCI-H460 Human Cell Line and bEnd.3 Mouse Cell Line antibody by Flow Cytometry.

Detection of DCBLD2/ESDN in NCI‑H460 Human Cell Line and bEnd.3 Mouse Cell Line by Flow Cytometry.

NCI-H460 human large cell lung carcinoma cell line (panel A) and bEnd.3 mouse endothelioma cell line (panel B) were stained with Sheep Anti-Human/Mouse DCBLD2/ESDN PE-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB6269P, filled histogram) or isotype control antibody (Catalog # IC016P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Applications for Human/Mouse DCBLD2/ESDN PE‑conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: NCI‑H460 human large cell lung carcinoma cell line and bEnd.3 mouse endothelioma cell line

Spectra Viewer

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: DCBLD2/ESDN

DCBLD2 (Discoidin, CUB and CLLC Domain-containing 2), also known as ESDN and CLCP1, is a novel, 95-130 kDa protein that bears strong resemblance to the neuropilin family of proteins. It is a type I transmembrane glycoprotein whose ORF contains an extremely long signal sequence of 66 amino acid (aa) length. Mature human DCBLD2 is 709 aa in length, and contains a 462 aa extracellular region that is characterized by the presence of one CUB and F5/8 (or discoidin) domain (and thus its resemblance to Neuropilin), plus one 93 aa LCCL domain. Over aa 67-528, human and mouse DCBLD2 share 87% aa sequence identity. Although DCBLD2 expression is widespread (if not ubiquitous), cell types specifically reported to contain DCBLD2 include skeletal muscle cells, vascular endothelial and smooth muscle cells, and various primary tumor cells. The large variation in native molecular weight is attributable to a variable glycosylation state. It would appear that the higher MW forms are more sensitive to ligation than the lower MW forms, and one possible ligand for DCBLD2 has been suggested to be SEMA4B. The exact nature of the DCBLD2 signaling complex is unclear; it has been proposed that multiple interacting partners exist in-cis, and may include InsRb, VEGFR2, and PEGFR beta. Notably, DCBLD2 appears to regulate the ubiquitination state of its receptor partners, and may either upregulate or downregulate receptor activity in a cell-specific manner.

Long Name

Discoidin, CUB and LCCL Domain Containing 2/Endothelial and Smooth Muscle Cell-derived Neuropilin-li

Alternate Names

CLCP1, ESDN

Entrez Gene IDs

131566 (Human); 73379 (Mouse); 155696 (Rat)

Gene Symbol

DCBLD2

UniProt

Q96PD2 (Human)

Additional DCBLD2/ESDN Products

Product Documents for Human/Mouse DCBLD2/ESDN PE‑conjugated Antibody

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Product Specific Notices for Human/Mouse DCBLD2/ESDN PE‑conjugated Antibody

For research use only

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