Human/Mouse DR3/TNFRSF25 Alexa Fluor® 647-conjugated Antibody

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R&D Systems Antibodies
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Human/Mouse DR3/TNFRSF25 Alexa Fluor® 647-conjugated Antibody Summary

Species Reactivity
Human, Mouse
Detects human DR3/TNFRSF25 in direct ELISAs and Western blots. Shows 100% cross-reactivity with recombinant mouse (rm) DR3/TNFRSF25 and no cross-reactivity with recombinant human (rh) 4-1BB, rhCD27, rhCD30, rhCD40, rhDR6, rhBAFF R, rhFas, rhGITR, rhLTR beta, rhNGF R, rhOPG, rmOX40, rhRANK, rhTAJ, rhTNF RI, rhTNF RII, or rhHVEM.
Monoclonal Mouse IgG1 Clone # 59204
Mouse myeloma cell line NS0-derived recombinant human DR3 isoform 1
Accession # Q93038
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Alexa Fluor 647 (Excitation= 650 nm, Emission= 668 nm)


Recommended Concentration
Flow Cytometry
0.25-1 µg/106 cells
Human peripheral blood mononuclear cells (PBMCs) treated with PMA and Calcium Ionomycin and mouse splenocytes treated with PMA and Calcium Ionomycin

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: DR3/TNFRSF25

Death receptor 3 (DR3), also known as lymphocyte-associated receptor of death (LARD), WSL-1, APO3, TRAMP and TR3, is a glycoprotein belonging to the TNF receptor superfamily (TNFRSF) (1 - 5). DR3 was formerly designated TNFRSF12 when it was thought to be a receptor for TWEAK/TNFSF12 (6). However, work disavowed the DR3:TWEAK interaction and DR3 is now designated TNFRSF 25 (7). By alternative splicing, at least 11 distinct human DR3 transcripts encoding secreted or type I membrane proteins exist (7). The human DR3 isoform 1 cDNA encodes a 417 amino acid residue (aa) transmembrane precursor with a 24 aa signal peptide, a 175 aa extracellular domain containing four cysteine-rich repeats and two potential N-glycosylation sites, a 21 aa transmembrane region and a 195 aa cytoplasmic region with one death domain. DR3 is one of six within the TNF R superfamily that contains a death domain in its cytooplasmic region. It is most closely related to TNF R1 and FAS/CD95, sharing 29% and 23% aa sequence identity, respectively. DR3 is expressed primarily in tissues enriched in lymphocytes. Whereas naïve B and T cells express multiple truncated DR3 isoforms but not the transmembrane isoform 1, upon T cell activation, expression of the transmembrane DR3 isoform 1 predominates. TL1A/VEGI, a TNF superfamily ligand, has been shown to bind and activate DR3 (8). Depending on the cell context, ligation of DR3 by TL1A can trigger one of two signaling pathways. On primary T cells, TL1A induces NF-kappa-B activation and a costimulatory signal to increase IL-2 responsiveness and the secretion of proinflammatory cytokines. However, in a tumor cell line, TF-1, TL1A has been shown to induce caspase activity and apoptosis. In DR3-null mice, an impairment of negative selection and anti-CD3-mediated thymocyte apoptosis is observed.

  1. Chinnaiyan, A.M. et al. (1996) Science 274:990.
  2. Kitson, J. et al. (1996) Nature 384:372.
  3. Bodmer, J-L. et al. (1997) Immunity 6:79.
  4. Screaton, G.R. et al. (1997) Proc. Nat. Acad. Sci. USA 94:4615.
  5. Marsters, S.A. et al. (1996) Curr. Biol. 6:1669.
  6. Marsters, S.A. et al. (1998) Curr. Biol. 8:525.
  7. Kaptein, A. et al. (2000) FEBS Lett. 485:135.
  8. Migone, T-S. et al. (2002) Immunity 16:479.
Long Name
Death Receptor 3
Entrez Gene IDs
8718 (Human); 85030 (Mouse)
Alternate Names
APO3; Apo-3; Apoptosis-inducing receptor AIR; Apoptosis-mediating receptor DR3; Apoptosis-mediating receptor TRAMP; Death receptor 3; DR3; DR3member 12 (translocatingchain-association membrane protein); LARD; Lymphocyte-associated receptor of death; Protein WSL; Protein WSL-1; TNFRSF25; TRAMP; tumor necrosis factor receptor superfamily, member 25; WSL; WSL1; WSL-1

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Product Specific Notices

This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.


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