Human/Mouse Frizzled-7 Antibody

Catalog # Availability Size / Price Qty
MAB1981-050
MAB1981-100
MAB1981-SP
Frizzled‑7 in HEK293 Human Cell Line.
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Product Details
Citations (5)
FAQs
Supplemental Products
Reviews (3)

Human/Mouse Frizzled-7 Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects human and mouse Frizzled-7. In direct ELISAs, no cross-reactivity with recombinant mouse Frizzled-1, -3, -4 or -8 is observed.
Source
Monoclonal Rat IgG2A Clone # 151143
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Frizzled-7
Gln33-Leu185
Accession # Q61090
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
2.5 µg/106 cells
Retinoic acid‑treated D3 mouse embryonic stem cell line
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry Frizzled-7 antibody in HEK293 Human Cell Line by Immunocytochemistry (ICC). View Larger

Frizzled‑7 in HEK293 Human Cell Line. Frizzled-7 was detected in immersion fixed HEK293 human embryonic kidney cell line using Rat Anti-Human/Mouse Frizzled-7 Monoclonal Antibody (Catalog # MAB1981) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI(blue). Specific staining was localized to the cell surface. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Frizzled-7

Frizzled-7 is a member of the Frizzled family of unconventional G-protein-coupled glycoprotein receptors for the Wnt signaling pathway (1‑3). The Wnt genes encode a large family of glycoproteins that are essential in development and tissue maintenance (1, 2). Like other Frizzled family members, Frizzled-7 contains a divergent N‑terminal signal peptide (amino acid (aa) 1‑32), a highly conserved extracellular cysteine-rich domain (CRD, aa 44‑169), a variable-length linker region (aa 170‑256), a seven-pass transmembrane region (aa 257‑549), and a variable-length C-terminal cytoplasmic domain (aa 550‑574) (1‑3). The CRD, which comprises the binding site for Wnts and other ligands such as Syndecan 4 and fibronectin, spans about 130 amino acid residues and contains ten invariant cysteine residues (2, 3). Expressed alone, it can compete with native Frizzled to inhibit Wnt canonical signaling (4). Within aa 33‑185, human Frizzled-7 shares ~99% aa identity with human, rat, canine and bovine Frizzled-7. Mature Frizzled-7 also shares 80% aa identity with Frizzled-1 and Frizzled-2. Roles for Frizzled-7 have been determined in both canonical Wnt/ beta ‑Catenin-mediated signaling and non‑canonical planar cell polarity and calcium pathways (1, 2, 4). During development, Frizzled-7 is expressed during gastrulation and in the fetal gut, kidney and lung where it is thought to influence tissue morphogenesis via non-canonical signaling pathways (3‑5). In the adult, Frizzled-7 is expressed in skeletal muscle, especially in satellite cells that mediate muscle regeneration in response to Wnt-7a (3, 6). It is expressed in embryonic stem cells (ES), contributing to self-renewal signaling (7). It has been implicated in mesenchymal-to-epithelial transition in colorectal cancer (2, 8). Frizzled-7 mRNA has also been detected in adult heart and placenta (3).

References
  1. Schulte, G. and V. Bryja (2007) Trends Pharmacol. Sci. 28:518.
  2. Vincan, E. et al. (2007) Front. Biosci. 12:4558.
  3. Sagara, N. et al. (1998) Biochem. Biophys. Res. Commun. 252:117.
  4. Medina, A. et al. (2000) Mech. Dev. 92:227.
  5. Kemp, C.R. et al. (2007) Dev. Dyn. 236:2011.
  6. Le Grand, F. et al. (2009) Cell Stem Cell 4:535.
  7. Melchior, K. et al. (2008) Biol. Chem. 389:897.
  8. Vincan, E. et al. (2007) Oncogene 26:2340.
Entrez Gene IDs
8324 (Human); 14369 (Mouse)
Alternate Names
frizzled (Drosophila) homolog 7; frizzled homolog 7 (Drosophila); Frizzled, drosophila, homolog of, 7; Frizzled7; Frizzled-7; fz-7; FZD7; FzE3frizzled-7; hFz7

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Citations for Human/Mouse Frizzled-7 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. Mutant APC reshapes Wnt signaling plasma membrane nanodomains by altering cholesterol levels via oncogenic beta -catenin
    Authors: Alfredo Erazo-Oliveras, Mónica Muñoz-Vega, Mohamed Mlih, Venkataramana Thiriveedi, Michael L. Salinas, Jaileen M. Rivera-Rodríguez et al.
    Nature Communications
  2. Canonical and non-canonical Wnt signaling are simultaneously activated by Wnts in colon cancer cells
    Authors: E Flores-Her, DM Velázquez, MC Castañeda-, G Fuentes-Ga, G Fonseca-Ca, JK Yamamoto-F, MT Romero-Avi, JA García-Sái, M Robles-Flo
    Cell. Signal., 2020-04-10;72(0):109636.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Antibody-directed lentiviral gene transduction for live-cell monitoring and selection of human iPS and hES cells.
    Authors: Wu DT, Seita Y, Zhang X
    PLoS ONE, 2012-04-20;7(4):e34778.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. Noncanonical Wnt signaling orchestrates early developmental events toward hematopoietic cell fate from human embryonic stem cells.
    Authors: Vijayaragavan K, Szabo E, Bosse M, Ramos-Mejia V, Moon RT, Bhatia M
    Cell Stem Cell, 2009-03-06;4(3):248-62.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. Cross-talk between Wnt signaling pathways in human mesenchymal stem cells leads to functional antagonism during osteogenic differentiation.
    Authors: Baksh D, Boland GM, Tuan RS
    J. Cell. Biochem., 2007-08-01;101(5):1109-24.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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Reviews for Human/Mouse Frizzled-7 Antibody

Average Rating: 5 (Based on 3 Reviews)

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Human/Mouse Frizzled-7 Antibody
By Anonymous on 08/17/2021
Application: WB Sample Tested: HEK293 human embryonic kidney cell line Species: Human and human eyes

1:1000 dilution worked very well


Human/Mouse Frizzled-7 Antibody
By Mike van Nijmegen on 08/06/2021
Application: IHC Sample Tested: mouse eye Species: Mouse

dilution 1:100-1:200


Human/Mouse Frizzled-7 Antibody
By Anonymous on 07/21/2021
Application: Immunocytochemistry/Immunofluorescence Sample Tested: limbal stem cells Species: Human and Mouse

It works very well with 1:200 dilution.