Frizzled-7 Antibody (151143)

R&D Systems | Catalog # MAB1981

R&D Systems
Discontinued Product
MAB1981 has been discontinued. View all Frizzled-7 products.

Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Transgenic Mouse

Applications

Validated:

Flow Cytometry, Immunocytochemistry, CyTOF-ready

Cited:

Immunohistochemistry, Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 151143
View all Frizzled-7 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Frizzled-7
Gln33-Leu185
Accession # Q61090

Specificity

Detects human and mouse Frizzled-7. In direct ELISAs, no cross-reactivity with recombinant mouse Frizzled-1, -3, -4 or -8 is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Scientific Data Images for Frizzled-7 Antibody (151143)

Frizzled-7 antibody in HEK293 Human Cell Line by Immunocytochemistry (ICC).

Frizzled‑7 in HEK293 Human Cell Line.

Frizzled-7 was detected in immersion fixed HEK293 human embryonic kidney cell line using Rat Anti-Human/Mouse Frizzled-7 Monoclonal Antibody (Catalog # MAB1981) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI(blue). Specific staining was localized to the cell surface. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Frizzled-7 Antibody (151143)

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

2.5 µg/106 cells
Sample: Retinoic acid‑treated D3 mouse embryonic stem cell line

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed HEK293 human embryonic kidney cell line

Reviewed Applications

Read 3 reviews rated 5 using MAB1981 in the following applications:

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Frizzled-7

Frizzled-7 is a member of the Frizzled family of unconventional G-protein-coupled glycoprotein receptors for the Wnt signaling pathway (1‑3). The Wnt genes encode a large family of glycoproteins that are essential in development and tissue maintenance (1, 2). Like other Frizzled family members, Frizzled-7 contains a divergent N‑terminal signal peptide (amino acid (aa) 1‑32), a highly conserved extracellular cysteine-rich domain (CRD, aa 44‑169), a variable-length linker region (aa 170‑256), a seven-pass transmembrane region (aa 257‑549), and a variable-length C-terminal cytoplasmic domain (aa 550‑574) (1‑3). The CRD, which comprises the binding site for Wnts and other ligands such as Syndecan 4 and fibronectin, spans about 130 amino acid residues and contains ten invariant cysteine residues (2, 3). Expressed alone, it can compete with native Frizzled to inhibit Wnt canonical signaling (4). Within aa 33‑185, human Frizzled-7 shares ~99% aa identity with human, rat, canine and bovine Frizzled-7. Mature Frizzled-7 also shares 80% aa identity with Frizzled-1 and Frizzled-2. Roles for Frizzled-7 have been determined in both canonical Wnt/ beta ‑Catenin-mediated signaling and non‑canonical planar cell polarity and calcium pathways (1, 2, 4). During development, Frizzled-7 is expressed during gastrulation and in the fetal gut, kidney and lung where it is thought to influence tissue morphogenesis via non-canonical signaling pathways (3‑5). In the adult, Frizzled-7 is expressed in skeletal muscle, especially in satellite cells that mediate muscle regeneration in response to Wnt-7a (3, 6). It is expressed in embryonic stem cells (ES), contributing to self-renewal signaling (7). It has been implicated in mesenchymal-to-epithelial transition in colorectal cancer (2, 8). Frizzled-7 mRNA has also been detected in adult heart and placenta (3).

References

  1. Schulte, G. and V. Bryja (2007) Trends Pharmacol. Sci. 28:518.
  2. Vincan, E. et al. (2007) Front. Biosci. 12:4558.
  3. Sagara, N. et al. (1998) Biochem. Biophys. Res. Commun. 252:117.
  4. Medina, A. et al. (2000) Mech. Dev. 92:227.
  5. Kemp, C.R. et al. (2007) Dev. Dyn. 236:2011.
  6. Le Grand, F. et al. (2009) Cell Stem Cell 4:535.
  7. Melchior, K. et al. (2008) Biol. Chem. 389:897.
  8. Vincan, E. et al. (2007) Oncogene 26:2340.

Alternate Names

Frizzled7, FZD7

Entrez Gene IDs

8324 (Human); 14369 (Mouse)

Gene Symbol

FZD7

UniProt

Q61090

Additional Frizzled-7 Products

Product Documents for Frizzled-7 Antibody (151143)

Certificate of Analysis

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Product Specific Notices for Frizzled-7 Antibody (151143)

For research use only

Citations for Frizzled-7 Antibody (151143)

Customer Reviews for Frizzled-7 Antibody (151143) (3)

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3 Customer Ratings
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Customer Images

Showing  1 - 3 of 3 reviews Showing All
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  • Human/Mouse Frizzled-7 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: HEK293 human embryonic kidney cell line
    Species: human eyes and Human
    Verified Customer | Posted 08/17/2021
    1:1000 dilution worked very well
    Frizzled-7 Antibody (151143) MAB1981
  • Human/Mouse Frizzled-7 Antibody
    Name: Mike van Nijmegen
    Application: Immunohistochemistry
    Sample Tested: mouse eye
    Species: Mouse
    Verified Customer | Posted 08/06/2021
    Dilution 1:100-1:200
    Frizzled 7 was detected in the limbus.
    Frizzled-7 Antibody (151143) MAB1981
  • Human/Mouse Frizzled-7 Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: limbal stem cells
    Species: Human and Mouse
    Verified Customer | Posted 07/21/2021
    It works very well with 1:200 dilution.
    Frizzled-7 Antibody (151143) MAB1981

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