Human/Mouse Frizzled-7 Antibody

(3 citations)   
  • Species Reactivity
    Human, Mouse
  • Specificity
    Detects human and mouse Frizzled-7. In direct ELISAs, no cross-reactivity with recombinant mouse Frizzled-1, -3, -4 or -8 is observed.
  • Source
    Monoclonal Rat IgG2A Clone # 151143
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse Frizzled-7
    Gln33-Leu185
    Accession # Q61090
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    2.5 µg/106 cells
    Retinoic acid‑treated D3 mouse embryonic stem cell line
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
  • Immunocytochemistry
    8-25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunocytochemistry
Frizzled‑7 in HEK293 Human Cell Line. Frizzled‑7 was detected in immersion fixed HEK293 human embryonic kidney cell line using Rat Anti-Human/Mouse Frizzled‑7 Monoclonal Antibody (Catalog # MAB1981) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI(blue). Specific staining was localized to the cell surface. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Frizzled-7
Frizzled-7 is a member of the Frizzled family of unconventional G-protein-coupled glycoprotein receptors for the Wnt signaling pathway (1‑3). The Wnt genes encode a large family of glycoproteins that are essential in development and tissue maintenance (1, 2). Like other Frizzled family members, Frizzled-7 contains a divergent N‑terminal signal peptide (amino acid (aa) 1‑32), a highly conserved extracellular cysteine-rich domain (CRD, aa 44‑169), a variable-length linker region (aa 170‑256), a seven-pass transmembrane region (aa 257‑549), and a variable-length C-terminal cytoplasmic domain (aa 550‑574) (1‑3). The CRD, which comprises the binding site for Wnts and other ligands such as Syndecan 4 and fibronectin, spans about 130 amino acid residues and contains ten invariant cysteine residues (2, 3). Expressed alone, it can compete with native Frizzled to inhibit Wnt canonical signaling (4). Within aa 33‑185, human Frizzled-7 shares ~99% aa identity with human, rat, canine and bovine Frizzled-7. Mature Frizzled-7 also shares 80% aa identity with Frizzled-1 and Frizzled-2. Roles for Frizzled-7 have been determined in both canonical Wnt/ beta ‑Catenin-mediated signaling and non‑canonical planar cell polarity and calcium pathways (1, 2, 4). During development, Frizzled-7 is expressed during gastrulation and in the fetal gut, kidney and lung where it is thought to influence tissue morphogenesis via non-canonical signaling pathways (3‑5). In the adult, Frizzled-7 is expressed in skeletal muscle, especially in satellite cells that mediate muscle regeneration in response to Wnt-7a (3, 6). It is expressed in embryonic stem cells (ES), contributing to self-renewal signaling (7). It has been implicated in mesenchymal-to-epithelial transition in colorectal cancer (2, 8). Frizzled-7 mRNA has also been detected in adult heart and placenta (3).
  • References:
    1. Schulte, G. and V. Bryja (2007) Trends Pharmacol. Sci. 28:518.
    2. Vincan, E. et al. (2007) Front. Biosci. 12:4558.
    3. Sagara, N. et al. (1998) Biochem. Biophys. Res. Commun. 252:117.
    4. Medina, A. et al. (2000) Mech. Dev. 92:227.
    5. Kemp, C.R. et al. (2007) Dev. Dyn. 236:2011.
    6. Le Grand, F. et al. (2009) Cell Stem Cell 4:535.
    7. Melchior, K. et al. (2008) Biol. Chem. 389:897.
    8. Vincan, E. et al. (2007) Oncogene 26:2340.
  • Entrez Gene IDs:
    8324 (Human); 14369 (Mouse)
  • Alternate Names:
    frizzled (Drosophila) homolog 7; frizzled homolog 7 (Drosophila); Frizzled, drosophila, homolog of, 7; Frizzled7; Frizzled-7; fz-7; FZD7; FzE3frizzled-7; hFz7
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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Species
Applications
Sample Type
  1. Antibody-directed lentiviral gene transduction for live-cell monitoring and selection of human iPS and hES cells.
    Authors: Wu DT, Seita Y, Zhang X
    PLoS ONE, 2012;7(4):e34778.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  2. Noncanonical Wnt signaling orchestrates early developmental events toward hematopoietic cell fate from human embryonic stem cells.
    Authors: Vijayaragavan K, Szabo E, Bosse M, Ramos-Mejia V, Moon RT, Bhatia M
    Cell Stem Cell, 2009;4(3):248-62.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  3. Cross-talk between Wnt signaling pathways in human mesenchymal stem cells leads to functional antagonism during osteogenic differentiation.
    Authors: Baksh D, Boland GM, Tuan RS
    J. Cell. Biochem., 2007;101(5):1109-24.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
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