Human/Mouse Glucokinase/GCK Alexa Fluor™ Plus 555‑conjugated Antibody

R&D Systems | Catalog # FAB7840AFP555

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Key Product Details

Species Reactivity

Human, Mouse

Applications

Western Blot

Label

Alexa Fluor Plus 555 (Excitation = 558 nm, Emission = 572 nm)

Antibody Source

Monoclonal Mouse IgG1 Clone # 849520
View all Glucokinase/GCK Primary Antibodies »

Product Specifications

Specificity

Detects human Glucokinase/GCK in ELISAs. In direct ELISAs, no cross-reactivity with recombinant human PRF-1 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Applications for Human/Mouse Glucokinase/GCK Alexa Fluor™ Plus 555‑conjugated Antibody

Application
Recommended Usage

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: Glucokinase/GCK

Hexokinases phosphorylate hexose to form hexose 6‑phosphate, the first step for hexose metabolism. There are four mammalian hexokinases (I, II, III and IV) and hexokinase IV is commonly known as glucose kinase (GCK). Unlike hexokinase I, II and III, which have high affinity for glucose and are strongly inhibited by the product, glucose‑6‑phosphate (1), GCK has much lower affinity for glucose and is not inhibited by the product (2, 3). Consequently, GCK has a Km for glucose of approximately 7 mM (4), which is 100 times higher than that of hexokinase I, II, and III. This unique enzymatic property of GCK allows it to respond to blood glucose levels and contribute to the maintenance of blood glucose levels within the normal physiological range of 4 mM to 6 mM. In the pancreatic islets, GCK serves as a glucose sensor to control insulin release in the beta cells, and to control glucagon release in the alpha cells (5). In hepatocytes, GCK responds to changes of ambient glucose levels by increasing or reducing glycogen synthesis. Mutations in GCK have been associated with non‑insulin‑dependent diabetes mellitus (6, 7), maturity‑onset diabetes of the young type 2 (8), and hyperinsulinemia of infancy (9). The enzyme activity was measured using a phosphatase coupled kinase assay (4).

References

  1. Aleshin, A.E. et al.(1998) Structure 6:39.
  2. Takeda, J. et al. (1993) J. Biol. Chem. 268:15200.
  3. Lange, A.J. et al. (1991) Biochem. J. 277:159.
  4. Wu, Z.L. (2011) PLoS ONE 6(8):e23172.
  5. Xu, L.Z. et al. (1995) J. Biol. Chem. 270:9939.
  6. Gidh-Jain, M. et al. (1993) Proc. Natl. Acad. Sci. U. S. A. 90:1932.
  7. Stoffel, M. et al. (1992) Proc. Natl. Acad. Sci. U.S.A. 89:7698.
  8. Hattersley, A.T. et al. (1992) Lancet 339:1307.
  9. Gloyn, A.L. et al. (2003) Diabetes 52:2433.

Long Name

Glucose Kinase

Alternate Names

FGQTL3, GCK, GLK, Hexokinase-4, Hexokinase-D, HHF3, HK4, HXKP, LGLK

Entrez Gene IDs

2645 (Human); 103988 (Mouse); 24385 (Rat)

Gene Symbol

GCK

UniProt

P35557 (Human)

Additional Glucokinase/GCK Products

Product Documents for Human/Mouse Glucokinase/GCK Alexa Fluor™ Plus 555‑conjugated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human/Mouse Glucokinase/GCK Alexa Fluor™ Plus 555‑conjugated Antibody


This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

For research use only

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