Detection of Jagged 1/Jagged 2 in HUVEC Human Cells by Flow Cytometry.
HUVEC human umbilical vein endothelial cells were stained with Mouse Anti-Human/Mouse Jagged 1/Jagged 2 PE‑conjugated Monoclonal Antibody (Catalog # FAB1726P, filled histogram) or isotype control antibody (Catalog # IC0041P, open histogram). View our protocol for Staining Membrane-associated Proteins.
Detection of Jagged 1/Jagged 2 in D3 Mouse Cell Line by Flow Cytometry.
D3 mouse embryonic stem cell line was stained with Mouse Anti-Human/Mouse Jagged 1/Jagged 2 PE‑conjugated Monoclonal Antibody (Catalog # FAB1726P, filled histogram) or isotype control antibody (Catalog # IC0041P, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
Background: Jagged 1/Jagged 2
Human Jagged 2 is a 131 kDa (predicted) member of the Delta-Serrate-Lag-2 (DSL) family of ligands. This family activates LIN12/Notch proteins and thereby regulates cell fate determination during development (1-5). It is a type 1 transmembrane protein that is synthesized as a 1238 amino acid (aa) precursor. It contains a 23 aa signal sequence, a large 1057 aa extracellular region, a 21 aa transmembrane region, and a short 137 aa cytoplasmic region. The extracellular region contains four potential N-linked glycosylation sites, a DSL domain, 16 EGF-like repeats (many of which are also sites of calcium binding), a von Willebrand factor (vWF) type C domain, and a cysteine-rich region just proximal to the transmembrane segment (2). There are two isoforms for human Jagged 2, named long and short. The short form lacks a splicing variant region (aa 421-461) that is present in the long form of the protein. Human Jagged 2 shares 90% and 87% aa sequence identity with mouse and rat Jagged 2, respectively. During murine embryonic development, Jagged 2 is expressed highest in fetal thymus, epidermis, foregut, dorsal root ganglia, and inner ear (2). In 2 week old mice, the Jagged 2 transcript is most abundant in heart, lung, thymus, skeletal muscle, brain, and testis (2). Functionally, it is suggested that Jagged 2 engages the Notch1 pathway of signal transduction (2). It is involved in the development of the mammalian limb, branchial arches, central and peripheral nervous systems, T cell lineage differentiation, natural killer cells, and the establishment of functional natural killer cell lines (3, 5, 6).
Shawber, C. et al. (1996) Dev. Biol. 180:370.
Luo, B. et al. (1997) Mol. Cell. Biol. 17:6057.
Valsecchi, V. et al. (1997) Mech. Dev. 69:203.
Schickwann, T. et al. (2000) Blood 96:950.
DeHart, S. et al. (2005) Blood 105:3521.
de La Coste, A. and A.A. Freitas (2006) Immunol. Lett. 102:1.
Have you used Human/Mouse Jagged 1/Jagged 2 PE-conjugated Antibody?
Submit a review and receive a $25US/€18/£15/$25CAN amazon gift card if you include an image - $10US/€7/£6/$10CAN Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.