Human/Mouse/Rat Androgen R/NR3C4 PE‑conjugated Antibody

R&D Systems | Catalog # IC5876P

R&D Systems
Discontinued Product
IC5876P has been discontinued. View all Androgen R/NR3C4 products.

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human

Applications

Validated:

Intracellular Staining by Flow Cytometry

Cited:

Immunocytochemistry

Label

Phycoerythrin (Excitation = 488 nm, Emission = 565-605 nm)

Antibody Source

Monoclonal Mouse IgG2B Clone # 523339
View all Androgen R/NR3C4 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Androgen R/NR3C4
Thr660-Gln919
Accession # P10275

Specificity

Detects human Androgen R/NR3C4 in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human/Mouse/Rat Androgen R/NR3C4 PE‑conjugated Antibody

Detection of Androgen R/NR3C4 antibody in LNCaP Human Cell Line antibody by Flow Cytometry.

Detection of Androgen R/NR3C4 in LNCaP Human Cell Line by Flow Cytometry.

LNCaP human prostate cancer cell line was stained with Mouse Anti-Human/Mouse/Rat Androgen R/NR3C4 PE-conjugated Monoclonal Antibody (Catalog # IC5876P, filled histogram) or isotype control antibody (Catalog # IC0041P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Applications for Human/Mouse/Rat Androgen R/NR3C4 PE‑conjugated Antibody

Application
Recommended Usage

Intracellular Staining by Flow Cytometry

10 µL/106 cells
Sample: LNCaP human prostate cancer cell line fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: Androgen R/NR3C4

AR (Androgen Receptor), also known as NR3C4, is a 99 kDa (predicted) member of the NR3 subfamily, nuclear hormone receptor family of proteins. Due to a high number of Gln and Pro residues, it runs anomalously at 100‑120 kDa in SDS-PAGE. It is widely expressed, being found in neurons, endothelial cells, osteoblasts, chrondrocytes, macrophages, adipocytes, and prostate epithelium. Human AR is 919 amino acids (aa) in length. It contains three discrete domains: a "modulating" N‑terminus (aa 1‑553) that is rich in Gln, Pro and Gly, a Zn-finger DNA-binding region (aa 554‑635), and a ligand-binding domain (aa 672‑917). AR is highly polymorphic at the N‑terminus, with total Gln and Gly residues differing by seven or more residues among individuals. Multiple potential splice forms exist, including an alternative start site at Met189 and a seven aa substitution for aa 1‑538 that generates a 45 kDa isoform. AR does homodimerize, apparently with multiple isotypes. The ligand binding domain of human AR (aa 661-920) shares 100% aa sequence identity with mouse and rat AR.

Long Name

Androgen Receptor

Alternate Names

AIS, AndrogenR, AR, DHTR, NR3C4, SBMA, SMAX1

Entrez Gene IDs

367 (Human)

Gene Symbol

AR

UniProt

P10275

Additional Androgen R/NR3C4 Products

Product Documents for Human/Mouse/Rat Androgen R/NR3C4 PE‑conjugated Antibody

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Product Specific Notices for Human/Mouse/Rat Androgen R/NR3C4 PE‑conjugated Antibody

For research use only

Citations for Human/Mouse/Rat Androgen R/NR3C4 PE‑conjugated Antibody

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Associated Pathways

TGF-beta Signaling Pathways TGF-beta Signaling Pathway Thumbnail