Human/Mouse/Rat/Chicken A2B5 Alexa Fluor® 488-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB1416G-100UG
Detection  of A2B5 in NTera-2 Human Cell Line by Flow Cytometry.
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Human/Mouse/Rat/Chicken A2B5 Alexa Fluor® 488-conjugated Antibody Summary

Species Reactivity
Human, Mouse, Rat, Chicken
Specificity
Recognizes the A2B5 cell surface ganglioside epitope found on type II astrocytes, cells involved in gliogenesis, and cells committed to the oligodendrocyte lineage (1‑4).
Source
Monoclonal Mouse IgM Clone # 105
Purification
IgM-specific Affinity-purified from hybridoma culture supernatant
Immunogen
Embryonic chicken retinal cells
Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 488

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25-1 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection  of A2B5 in NTera-2 Human Cell Line by Flow Cytometry. View Larger

Detection of A2B5 in NTera-2 Human Cell Line by Flow Cytometry. NTera‑2 human testicular embryonic carcinoma cell line either treated with 10 μM retinoic acid for 4 days (filled histogram) or untreated (open histogram) was stained with Mouse Anti-Human/Mouse/Rat/Chicken A2B5 Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # FAB1416G). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: A2B5

A2B5 is a cell surface ganglioside epitope expressed in developing thymic epithelial cells, oligodendrocyte progenitors, and neuroendocrine cells (1).

References
  1. Eisenbarth, G.S. et al. (1979) Proc. Natl. Acad. Sci. USA 76:4913.
  2. Bottenstein, J.E. et al. (1988) J. Neurosci. Res. 20:291.
  3. Suzumura, A. and D.H. Silberberg (1989) Brain Res. 480:51.
  4. Dubois-Dalcq, M. et al. (1990) Cellular and Molecular Biology of Myelination, Springer-Verlag, pp. 3.
Alternate Names
A2B5

Product Datasheets

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