Human/Mouse/Rat/Chicken A2B5 Alexa Fluor® 488-conjugated Antibody

• Species Reactivity
  Human, Mouse, Rat, Chicken
• Specificity
  Recognizes the A2B5 cell surface ganglioside epitope found on type II astrocytes, cells involved in gliogenesis, and cells committed to the oligodendrocyte lineage (1‑4).
• Source
  Monoclonal Mouse IgM Clone # 105
• Purification
  IgM-specific Affinity-purified from hybridoma culture supernatant
• Immunogen
  Embryonic chicken retinal cells
• Formulation
  Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
• Label
  Alexa Fluor 488

• Flow Cytometry
  0.25-1 µg/10⁶ cells
  See below

Flow Cytometry click image to view larger

Detection of A2B5 in NTera-2 Human Cell Line by Flow Cytometry. NTera‑2 human testicular embryonic carcinoma cell line either treated with 10 μM retinoic acid for 4 days (filled histogram) or untreated (open histogram) was stained with Mouse Anti-Human/Mouse/Rat/Chicken A2B5 Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # FAB1416G). View our protocol for Staining Membrane-associated Proteins.

• Shipping
  The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
• Stability & Storage
  Protect from light. Do not freeze.

  • 12 months from date of receipt, 2 to 8 °C as supplied.

• References:
  1. Eisenbarth, G.S. et al. (1979) Proc. Natl. Acad. Sci. USA 76:4913.
  2. Bottenstein, J.E. et al. (1988) J. Neurosci. Res. 20:291.
  3. Suzumura, A. and D.H. Silberberg (1989) Brain Res. 480:51.
  4. Dubois-Dalcq, M. et al. (1990) Cellular and Molecular Biology of Myelination, Springer-Verlag, pp. 3.

• Alternate Names:
  A2B5

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