Human/Mouse/Rat/Chicken A2B5 Alexa Fluor® 488-conjugated Antibody

Catalog # Availability Size / Price Qty
Detection of A2B5 in NTera-2 Human Cell Line by Flow Cytometry.
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Product Details
Citations (1)
Supplemental Products

Human/Mouse/Rat/Chicken A2B5 Alexa Fluor® 488-conjugated Antibody Summary

Species Reactivity
Human, Mouse, Rat, Chicken
Recognizes the A2B5 cell surface ganglioside epitope found on type II astrocytes, cells involved in gliogenesis, and cells committed to the oligodendrocyte lineage (1‑4).
Monoclonal Mouse IgM Clone # 105
IgM-specific Affinity-purified from hybridoma culture supernatant
Embryonic chicken retinal cells
Supplied in a saline solution containing BSA and Sodium Azide.
Alexa Fluor 488 (Excitation= 488 nm, Emission= 515-545 nm)


Recommended Concentration
Flow Cytometry
0.25-1 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of A2B5 antibody in NTera-2 Human Cell Line antibody by Flow Cytometry. View Larger

Detection of A2B5 in NTera-2 Human Cell Line by Flow Cytometry. NTera‑2 human testicular embryonic carcinoma cell line either treated with 10 μM retinoic acid for 4 days (filled histogram) or untreated (open histogram) was stained with Mouse Anti-Human/Mouse/Rat/Chicken A2B5 Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # FAB1416G). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: A2B5

A2B5 is a cell surface ganglioside epitope expressed in developing thymic epithelial cells, oligodendrocyte progenitors, and neuroendocrine cells (1).

  1. Eisenbarth, G.S. et al. (1979) Proc. Natl. Acad. Sci. USA 76:4913.
  2. Bottenstein, J.E. et al. (1988) J. Neurosci. Res. 20:291.
  3. Suzumura, A. and D.H. Silberberg (1989) Brain Res. 480:51.
  4. Dubois-Dalcq, M. et al. (1990) Cellular and Molecular Biology of Myelination, Springer-Verlag, pp. 3.
Alternate Names
A2B5 marker; A2B5

Product Datasheets

Citation for Human/Mouse/Rat/Chicken A2B5 Alexa Fluor® 488-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Gene Expression Analysis of Astrocyte and Microglia Endocannabinoid Signaling during Autoimmune Demyelination
    Authors: Á Moreno-Gar, A Bernal-Chi, T Colomer, A Rodríguez-, C Matute, S Mato
    Biomolecules, 2020;10(9):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry


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