Human/Mouse/Rat/Chicken A2B5 Alexa Fluor® 488-conjugated Antibody

FAB1416G Datasheet / COA / SDS

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FAB1416G-100UG

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Detection of A2B5 in NTera-2 Human Cell Line by Flow Cytometry.

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Human/Mouse/Rat/Chicken A2B5 Alexa Fluor® 488-conjugated Antibody Summary

Species Reactivity

Human, Mouse, Rat, Chicken

Specificity

Recognizes the A2B5 cell surface ganglioside epitope found on type II astrocytes, cells involved in gliogenesis, and cells committed to the oligodendrocyte lineage (1‑4).

Source

Monoclonal Mouse IgM Clone # 105

Purification

IgM-specific Affinity-purified from hybridoma culture supernatant

Immunogen

Embryonic chicken retinal cells

Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Label

Alexa Fluor 488

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25-1 µg/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry

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Detection of A2B5 in NTera-2 Human Cell Line by Flow Cytometry. NTera‑2 human testicular embryonic carcinoma cell line either treated with 10 μM retinoic acid for 4 days (filled histogram) or untreated (open histogram) was stained with Mouse Anti-Human/Mouse/Rat/Chicken A2B5 Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # FAB1416G). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: A2B5

A2B5 is a cell surface ganglioside epitope expressed in developing thymic epithelial cells, oligodendrocyte progenitors, and neuroendocrine cells (1).

References

Eisenbarth, G.S. et al. (1979) Proc. Natl. Acad. Sci. USA 76:4913.
Bottenstein, J.E. et al. (1988) J. Neurosci. Res. 20:291.
Suzumura, A. and D.H. Silberberg (1989) Brain Res. 480:51.
Dubois-Dalcq, M. et al. (1990) Cellular and Molecular Biology of Myelination, Springer-Verlag, pp. 3.

Alternate Names

A2B5