Human/Mouse/Rat/Chicken A2B5 Alexa Fluor® 488-conjugated Antibody

    
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  • Species Reactivity
    Human, Mouse, Rat, Chicken
  • Specificity
    Recognizes the A2B5 cell surface ganglioside epitope found on type II astrocytes, cells involved in gliogenesis, and cells committed to the oligodendrocyte lineage (1‑4).
  • Source
    Monoclonal Mouse IgM Clone # 105
  • Purification
    IgM-specific Affinity-purified from hybridoma culture supernatant
  • Immunogen
    Embryonic chicken retinal cells
  • Formulation
    Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
  • Label
    Alexa Fluor 488
Product Datasheets

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Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    0.25-1 µg/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Example
Detection of A2B5 in NTera-2 Human Cell Line by Flow Cytometry. NTera‑2 human testicular embryonic carcinoma cell line either treated with 10 μM retinoic acid for 4 days (filled histogram) or untreated (open histogram) was stained with Mouse Anti-Human/Mouse/Rat/Chicken A2B5 Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # FAB1416G). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: A2B5
A2B5 is a cell surface ganglioside epitope expressed in developing thymic epithelial cells, oligodendrocyte progenitors, and neuroendocrine cells (1).
  • References:
    1. Eisenbarth, G.S. et al. (1979) Proc. Natl. Acad. Sci. USA 76:4913.
    2. Bottenstein, J.E. et al. (1988) J. Neurosci. Res. 20:291.
    3. Suzumura, A. and D.H. Silberberg (1989) Brain Res. 480:51.
    4. Dubois-Dalcq, M. et al. (1990) Cellular and Molecular Biology of Myelination, Springer-Verlag, pp. 3.
  • Alternate Names:
    A2B5
Related Research Areas

FAQs

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Isotype Controls
Description Application Cat# Citations Images  

Normal Mouse IgM Alexa Fluor® 488-conjugated Control

Ctrl IC015G
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Staining Reagents
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Flow Cytometry Staining Buffer (1X)

Flow FC001 10
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 5
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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