Detection of A2B5 in RAW 264.7 Mouse Cell Line by Flow Cytometry. NTera‑2 human testicular embryonic carcinoma cell line either treated with 10 μM retinoic acid for 4 days (filled histogram) or untreated (open histogram) was stained with Mouse Anti-Human/Mouse/Rat/Chicken A2B5 Monoclonal Antibody (Catalog # MAB1416), followed by Allophycocyanin-conjugated Anti-Mouse IgM Secondary Antibody (Catalog # F0117). View our protocol for Staining Membrane-associated Proteins.
A2B5 in NTera-2 Human Cell Line. A2B5 was detected in immersion fixed NTera‑2 human testicular embryonic carcinoma cell line (differentiated with retinoic acid for 10 days) using Mouse Anti-Human/Mouse/Rat/ Chicken A2B5 Monoclonal Antibody (Catalog # MAB1416) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgM Secondary Antibody (red; Catalog # NL019) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
A2B5 is a cell surface ganglioside epitope expressed in developing thymic epithelial cells, oligodendrocyte progenitors, and neuroendocrine cells (1).
Eisenbarth, G.S. et al. (1979) Proc. Natl. Acad. Sci. USA 76:4913.
Bottenstein, J.E. et al. (1988) J. Neurosci. Res. 20:291.
Suzumura, A. and D.H. Silberberg (1989) Brain Res. 480:51.
Dubois-Dalcq, M. et al. (1990) Cellular and Molecular Biology of Myelination, Springer-Verlag, pp. 3.
R&D Systems personnel manually curate a database that contains references using R&D Systems products.
The data collected includes not only links to publications in PubMed,
but also provides information about sample types, species, and experimental conditions.
The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.