Human/Mouse/Rat/Chicken A2B5 Antibody

(16 citations)   
  • Species Reactivity
    Human, Mouse, Rat, Chicken
  • Specificity
    Recognizes the A2B5 cell surface ganglioside epitope found on type II astrocytes, cells involved in gliogenesis, and cells committed to the oligodendrocyte lineage (1‑4).
  • Source
    Monoclonal Mouse IgM Clone # 105
  • Purification
    IgM-specific Affinity-purified from hybridoma culture supernatant
  • Immunogen
    Embryonic chicken retinal cells
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    0.25 µg/106 cells
    See below
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
  • Immunocytochemistry
    8-25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of A2B5 in NTera-2 Human Cell Line by Flow Cytometry. NTera‑2 human testicular embryonic carcinoma cell line either treated with 10 μM retinoic acid for 4 days (filled histogram) or untreated (open histogram) was stained with Mouse Anti-Human/Mouse/Rat/Chicken A2B5 Monoclonal Antibody (Catalog # MAB1416), followed by Allophycocyanin-conjugated Anti-Mouse IgM Secondary Antibody (Catalog # F0117). View our protocol for Staining Membrane-associated Proteins.
Immunocytochemistry
A2B5 in NTera-2 Human Cell Line. A2B5 was detected in immersion fixed NTera‑2 human testicular embryonic carcinoma cell line (differentiated with retinoic acid for 10 days) using Mouse Anti-Human/Mouse/Rat/
Chicken A2B5 Monoclonal Antibody (Catalog # MAB1416) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgM Secondary Antibody (red; Catalog # NL019) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: A2B5
A2B5 is a cell surface ganglioside epitope expressed in developing thymic epithelial cells, oligodendrocyte progenitors, and neuroendocrine cells (1).
  • References:
    1. Eisenbarth, G.S. et al. (1979) Proc. Natl. Acad. Sci. USA 76:4913.
    2. Bottenstein, J.E. et al. (1988) J. Neurosci. Res. 20:291.
    3. Suzumura, A. and D.H. Silberberg (1989) Brain Res. 480:51.
    4. Dubois-Dalcq, M. et al. (1990) Cellular and Molecular Biology of Myelination, Springer-Verlag, pp. 3.
  • Alternate Names:
    A2B5
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

16 Citations: Showing 1 - 10
Filter your results:

Species
Applications
Sample Type
  1. Lineage-Specific Differentiation Is Influenced by State of Human Pluripotency
    Authors: JH Lee, S Laronde, TJ Collins, Z Shapovalov, B Tanasijevi, JD McNicol, A Fiebig-Com, YD Benoit, JB Lee, RR Mitchell, M Bhatia
    Cell Rep, 2017;19(1):20-35.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  2. Characterization of oligodendroglial populations in mouse demyelinating disease using flow cytometry: clues for MS pathogenesis.
    Authors: Robinson A, Rodgers J, Goings G, Miller S
    PLoS ONE, 2015;9(9):e107649.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  3. Demyelination causes adult CNS progenitors to revert to an immature state and express immune cues that support their migration.
    Authors: Moyon S, Dubessy A, Aigrot M, Trotter M, Huang J, Dauphinot L, Potier M, Kerninon C, Melik Parsadaniantz S, Franklin R, Lubetzki C
    J Neurosci, 2015;35(1):4-20.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Frozen
  4. Differentiation of human umbilical cord matrix mesenchymal stem cells into neural-like progenitor cells and maturation into an oligodendroglial-like lineage.
    Authors: Leite C, Silva N, Mendes S, Ribeiro A, de Faria J, Lourenco T, dos Santos F, Andrade P, Cardoso C, Vieira M, Paiva A, da Silva C, Cabral J, Relvas J, Graos M
    PLoS ONE, 2014;9(10):e111059.
    Species: Mouse
    Sample Type: Whole Cells
    Application: IHC Fresh
  5. Generation of neuronal progenitor cells in response to tumors in the human brain.
    Authors: Macas J, Ku M, Nern C, Xu Y, Buhler H, Remke M, Synowitz M, Franz K, Seifert V, Plate K, Kettenmann H, Glass R, Momma S
    Stem Cells, 2014;32(1):244-57.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC
  6. Transcription factor-mediated reprogramming of fibroblasts to expandable, myelinogenic oligodendrocyte progenitor cells.
    Authors: Najm, Fadi J, Lager, Angela M, Zaremba, Anita, Wyatt, Krysta, Caprariello, Andrew V, Factor, Daniel C, Karl, Robert T, Maeda, Tadao, Miller, Robert H, Tesar, Paul J
    Nat Biotechnol, 2013;31(5):426-33.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  7. In vivo generation of neural tumors from neoplastic pluripotent stem cells models early human pediatric brain tumor formation.
    Authors: Werbowetski-Ogilvie TE, Morrison LC, Fiebig-Comyn A, Bhatia M
    Stem Cells, 2012;30(3):392-404.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  8. Disrupted-in-Schizophrenia-1 expression is regulated by beta-site amyloid precursor protein cleaving enzyme-1-neuregulin cascade.
    Authors: Seshadri S, Kamiya A, Yokota Y, Prikulis I, Kano S, Hayashi-Takagi A, Stanco A, Eom TY, Rao S, Ishizuka K, Wong P, Korth C, Anton ES, Sawa A
    Proc. Natl. Acad. Sci. U.S.A., 2010;107(12):5622-7.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  9. Musashi1 RNA-binding protein regulates oligodendrocyte lineage cell differentiation and survival.
    Authors: Dobson NR, Zhou YX, Flint NC, Armstrong RC
    Glia, 2008;56(3):318-30.
    Species: Rat
    Sample Type: Whole Cells
    Application: ICC
  10. Tim-2 is the receptor for H-ferritin on oligodendrocytes.
    Authors: Todorich B, Zhang X, Slagle-Webb B, Seaman WE, Connor JR
    J. Neurochem., 2008;107(6):1495-505.
    Species: Rat
    Sample Type: Whole Cells
    Application: ICC
  11. AMPA-mediated excitotoxicity in oligodendrocytes: role for Na(+)-K(+)-Cl(-) co-transport and reversal of Na(+)/Ca(2+) exchanger.
    Authors: Chen H, Kintner DB, Jones M, Matsuda T, Baba A, Kiedrowski L, Sun D
    J. Neurochem., 2007;102(6):1783-95.
    Species: Rat
    Sample Type: Whole Cells
    Application: ICC
  12. Cyclopamine treatment of human embryonic stem cells followed by culture in human astrocyte medium promotes differentiation into nestin- and GFAP-expressing astrocytic lineage.
    Authors: Lee DS, Yu K, Rho JY, Lee E, Han JS, Koo DB, Cho YS, Lee KK, Han YM
    Life Sci., 2006;80(2):154-9.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  13. Efficient induction of oligodendrocytes from human embryonic stem cells.
    Authors: Kang SM, Cho MS, Seo H, Yoon CJ, Oh SK, Choi YM, Kim DW
    Stem Cells, 2006;25(2):419-24.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  14. Excitatory amino acid induced oligodendrocyte cell death in vitro: receptor-dependent and -independent mechanisms.
    Authors: Rosin C, Bates TE, Skaper SD
    J. Neurochem., 2004;90(5):1173-85.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  15. High-level expression of functional chemokine receptor CXCR4 on human neural precursor cells.
    Authors: Ni HT, Hu S, Sheng WS, Olson JM, Cheeran MC, Chan AS, Lokensgard JR, Peterson PK
    Brain Res. Dev. Brain Res., 2004;152(2):159-69.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  16. Netrin 1 mediates spinal cord oligodendrocyte precursor dispersal.
    Authors: Tsai HH, Tessier-Lavigne M, Miller RH
    Development, 2003;130(10):2095-105.
    Species: Chicken
    Sample Type: Whole Cells
    Application: ICC
Expand to show all 16 Citations
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