Human/Mouse/Rat CLEC-2/CLEC1B Alexa Fluor™ Plus 555‑conjugated Antibody

R&D Systems | Catalog # AF1718AFP555

R&D Systems
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot

Label

Alexa Fluor Plus 555 (Excitation = 558 nm, Emission = 572 nm)

Antibody Source

Polyclonal Goat IgG
View all CLEC-2/CLEC1B Primary Antibodies »

Product Specifications

Specificity

Detects human, mouse, and rat CLEC-2/CLEC1B in Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant human CLEC-1 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications

Application
Recommended Usage

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: CLEC-2/CLEC1B

C-type lectin-like receptor 2 (CLEC-2) is a 32 kDa type II transmembrane glycoprotein and member of the C-type lectin-like family of receptors (1-4). CLEC-2 consists of a 33 amino acid (aa) cytoplasmic domain, a 21 aa transmembrane region, and a 175 aa extracellular domain. The cytoplasmic domain contains multiple threonine and serine residues which are sites of potential phosphorylation, and a YXXL (Tyr-Xaa-Xaa-Leu) motif through which CLEC-2 does its signaling (2, 4-5). Ligand binding and cross-linking of CLEC-2 induces Src kinase-dependent tyrosine phosphorylation of the YXXL sequence, inducing activation of the tyrosine kinase Syk and initiation of a signaling pathway that culminates in activation of phospholipase C gamma 2 (2, 5). The extracellular domain contains three potential sites of N-linked glycosylation, and a single carbohydrate recognition domain (CRD) which shows conservation of six cysteine residues (1, 6). Unlike most other members of the
C‑type lectin-like family of receptors, CLEC-2's CRD lacks the amino acid residues that are crucial for Ca2+-dependent carbohydrate binding, making it a
non‑classical C-type lectin receptor (1, 6). A splicing variant at aa 22-55 produces two isoforms for CLEC-2. Isoform 1 is the longer protein, and in isoform 2, an alanine residue is substituted for aa 22-55. Human CLEC-2 shares 63% aa sequence identity with mouse CLEC-2. CLEC-2 is expressed preferentially in liver, and is also detected in myeloid cells (monocytes, dendritic cells, and granulocytes) (1), platelets, and megakaryocytes (4). CLEC-2 is the receptor for the
platelet‑aggregating snake venom protein rhodocytin (3-4) and the molecule podoplanin, a transmembrane sialoglycoprotein that, when bound to CLEC-2, is involved in platelet aggregation, tumor metastasis, and lymphatic vessel formation (2, 7). CLEC-2 has also been shown to enhance infectivity of HIV-1 by mediating HIV-1 attachment and transfer by CLEC-2 transfected cells and platelets (8).

References

  1. Colonna, M. et al. (2000) Eur. J. Immunol. 30:697.
  2. Christou, C.M. et al. (2008) Biochem. J. 411:133.
  3. Watson, A.A. et al. (2007) J. Biol. Chem. 282:3165.
  4. Suzuki-Inoue, K. et al. (2006) Blood 107:542.
  5. Fuller, G.L. et al. (2007) J. Biol. Chem. 282:12397.
  6. Weis, W.I. et al. (1998) Immunol. Rev. 163:19.
  7. Suzuki-Inoue, K. et al. (2007) J. Biol. Chem. 282:25993.
  8. Chaipan, C. et al. (2006) J. Virol. 80:8951.

Long Name

C-type Lectin-like Receptor 2

Alternate Names

CLEC1B, CLEC2

Entrez Gene IDs

51266 (Human); 56760 (Mouse)

Gene Symbol

CLEC1B

UniProt

AAF36777

Additional CLEC-2/CLEC1B Products

Product Documents

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices


This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

For research use only

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