Human/Mouse/Rat Ezrin Alexa Fluor® 594-conjugated Antibody

Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
Product Details

Human/Mouse/Rat Ezrin Alexa Fluor® 594-conjugated Antibody Summary

Species Reactivity
Human, Mouse, Rat
Detects human Ezrin in direct ELISAs and detects human, mouse, and rat Ezrin in Western blots.
Monoclonal Rabbit IgG Clone # 2086C
E. coli-derived recombinant human Ezrin
Accession # P15311
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Alexa Fluor 594


Recommended Concentration
Intracellular Staining by Flow Cytometry
0.25-1 µg/106 cells
HeLa human cervical epithelial carcinoma cell line fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Ezrin

EZRIN (also Cytovillin, Villin2 and p81) is a founding member of the ERM family, Band 4.1 Superfamily of proteins. Although its predicted MW is 69 kDa, it runs anomalously at 77-82 kDa in SDS-PAGE. ERZIN is expressed by epithelial cells where it serves as a linker between the cell membrane and the actin cytoskeleton. Its presence is particularly strong in microvilli where it helps organize this structure. In addition, ERZIN also organizes microtubules in lymphocytes at or near the immunological synapse by interacting with Glg1. Human EZRIN is 585 amino acids (aa) in length. It contains a band 4.1 homology/FERM domain that binds CD44, ICAM-1, EBP50 and ERM family members (aa 1-295), a central alpha -helical region (aa 296-352), and a C-terminal ERM and actin-binding/FERM C domain (aa 353-586). EZRIN exists as either a monomer, or a homo/heterodimer. EZRIN is not constitutively active, but must be phosphorylated and unfolded to bind to cytoplasmic proteins. Over aa 438-562, human EZRIN shares 96% aa identity with mouse EZRIN.

Entrez Gene IDs
7430 (Human); 22350 (Mouse); 54319 (Rat)
Alternate Names
CVIL; CVL; Cytovillin 2; Cytovillin; DKFZp762H157; EZR; Ezrin; FLJ26216; p81; VIL2; VIL2MGC1584; villin 2 (ezrin); Villin 2; villin-2

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