Human/Mouse/Rat Kirrel3/NEPH2 Antibody

Detection of Rat Kirrel3 by Western Blot.

Western blot shows lysates of rat embryonic hippocampal neuron. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse/Rat Kirrel3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4910) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Kirrel3 at approximately 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Detection of Human Kirrel3/NEPH2 by Western Blot

Kirrel3 protein detection in adult human skeletal muscle. A – Western Blot results obtained with anti-human Kirrel3 antibody directed toward intracellular region of Kirrel3 on skeletal muscle lysates obtained from adult human males at baseline, days one and two after completion of a DHR exercise protocol. S = subject B – Western blot results from blocking peptide experiment. The antibody in A was incubated with blocking peptide before being added to membrane containing skeletal muscle lysates obtained from adult human males at baseline and one and two days post completion of a DHR exercise protocol. C – A second commercial antibody directed towards the extracellular domain of Kirrel3 was used on a selection of lysates obtained from adult human male skeletal muscle day1 or 2 post completion of the downhill running exercise bout. Ponceau staining was performed on membranes to demonstrate transfer and equal protein loading. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/25488023), licensed under a CC-BY license. Not internally tested by R&D Systems.