Detection of Rat Kirrel3 by Western Blot.
Western blot shows lysates of rat embryonic hippocampal neuron. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse/Rat Kirrel3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4910) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Kirrel3 at approximately 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Kirrel3 (Kin of IRRE-like protein 3 precursor; also NEPH2) is a 95-120 kDa member of a small family of podocin binding molecules. Kirrel3 is found on podocytes and olfactory sensory neurons and likely plays a role in cellular adhesion. Kirrel3 is 778 amino acids in length. It is a type I transmembrane (TM) glycoprotein that contains a 514 aa extracellular region (aa 22-535) with five C2 type Ig like domains (aa 48-515) plus a 222 aa cytoplasmic tail. Kirrel3 binds itself as well as nephrin, podocin and ZO-1. Kirrel3 is shed by MMPs, generating a soluble urinary fragment that is 25 kDa smaller than the TM form. Two variant isoforms exist. One has an alternate start site at Met34, while the second shows a 35 aa substitution for aa 566-778. Over aa 1-535, human Kirrel3 is 98% aa identical to mouse Kirrel3.
Kin of Irregular Chiasm-like 3
Entrez Gene IDs:
84623 (Human); 67703 (Mouse); 315546 (Rat)
KIAA1867MGC126824; kin of IRRE like 3 (Drosophila); Kin of irregular chiasm-like protein 3; kin of IRRE-like protein 3; KIRRE; Kirrel3; KIRREPRO4502; mKIRRE; MRD4; NEPH2; NEPH2MGC126850; nephrin-like 2; Nephrin-like protein 2
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