Human/Mouse/Rat Kirrel3/NEPH2 Antibody

(1 citations)   
  • Species Reactivity
    Human, Mouse, Rat
  • Specificity
    Detects Kirrel3/NEPH2 in direct ELISAs and Western blots. In direct ELISAs, less than 3% cross-reactivity with recombinant human (rh) Kirrel1 and rhKirrel2 is observed.
  • Source
    Polyclonal Sheep IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human Kirrel3/NEPH2
    Tyr33-Ala535
    Accession # Q8IZU9
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Rat Kirrel3 by Western Blot. Western blot shows lysates of rat embryonic hippocampal neuron. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse/Rat Kirrel3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4910) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Kirrel3 at approximately 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Kirrel3/NEPH2

Kirrel3 (Kin of IRRE-like protein 3 precursor; also NEPH2) is a 95-120 kDa member of a small family of podocin binding molecules. Kirrel3 is found on podocytes and olfactory sensory neurons and likely plays a role in cellular adhesion. Kirrel3 is 778 amino acids in length. It is a type I transmembrane (TM) glycoprotein that contains a 514 aa extracellular region (aa 22-535) with five C2 type Ig like domains (aa 48-515) plus a 222 aa cytoplasmic tail. Kirrel3 binds itself as well as nephrin, podocin and ZO-1. Kirrel3 is shed by MMPs, generating a soluble urinary fragment that is 25 kDa smaller than the TM form. Two variant isoforms exist. One has an alternate start site at Met34, while the second shows a 35 aa substitution for aa 566-778. Over aa 1-535, human Kirrel3 is 98% aa identical to mouse Kirrel3.

  • Long Name:
    Kin of Irregular Chiasm-like 3
  • Entrez Gene IDs:
    84623 (Human); 67703 (Mouse); 315546 (Rat)
  • Alternate Names:
    KIAA1867MGC126824; kin of IRRE like 3 (Drosophila); Kin of irregular chiasm-like protein 3; kin of IRRE-like protein 3; KIRRE; Kirrel3; KIRREPRO4502; mKIRRE; MRD4; NEPH2; NEPH2MGC126850; nephrin-like 2; Nephrin-like protein 2
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citations: Showing 1 - 1

  1. Identification of novel Kirrel3 gene splice variants in adult human skeletal muscle.
    Authors: Durcan, Peter, Conradie, Johannes, Van deVyver, Mari, Myburgh, Kathryn
    BMC Physiol, 2014;14(1):11.
    Species: Human
    Sample Type: Tissue Homogenates
    Application: WB
Isotype Controls
Description Application Cat# Citations Images  

Normal Sheep IgG Control

Ctrl 5-001-A 9
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....
Secondary Antibodies
Description Application Cat# Citations Images  

Sheep IgG HRP-conjugated Antibody

WB, Simple Western HAF016 14  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Donkey Anti-Sheep IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL010 3
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Donkey Anti-Sheep IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL011 5
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Donkey Anti-Sheep IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL012 2
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Sheep IgG (H+L) PE-conjugated Antibody

Flow F0126  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Donkey Anti-Sheep IgG Biotinylated Antibody

WB BAF016 2
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Sheep IgG (H+L) APC-conjugated Antibody

Flow F0127  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Sheep IgG VisUCyte HRP Polymer Antibody

IHC VC006  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Donkey Anti-Sheep IgG (H+L) Fluorescein-conjugated Antibody

Flow F0125 1
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Donkey Anti-Sheep IgG (H+L) PerCP-conjugated Antibody

Flow F0128
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Have you used Human/Mouse/Rat Kirrel3/NEPH2 Antibody?

Submit a review and receive a $25US/€18/£15/$25CAN amazon gift card if you include an image - $10US/€7/£6/$10CAN Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.

Order Details

Contact Information

Calculators

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷
R&D Systems Guarantee

Monoclonal
Antibody
Panels For
Screening

Learn More
Monoclonal Antibodies

Feedback

Customer Information