MIB1 (Mind bomb 1; also DIP-1 and ZZANK2) is a 110-115 kDa E3 ubiquitin ligase. It is widely expressed in both embryo and adult, and appears to have a non-redundant role in Notch receptor signaling. Notch signaling is initiated when a transmembrane (TM) Notch ligand (Jagged or Delta) binds to Notch on an adjacent cell. This ligation induces cleavage of the Notch extracellular domain (ECD) with the creation of a Jagged/Delta:NotchECD complex. This complex is now internalized by the Notch ligand-expressing cell. The displacement of the NotchECD from its TM segment promotes further Notch processing, resulting in Notch signaling. The key internalization step by the ligand-expressing cell is mediated by the MIB1 E3 ubiquitin ligase. Human MIB1 is 1006 amino acids (aa) in length. It contains multiple interspersed Ankyrin repeats, MIB domains, and zinc-finger motifs. There is one potential alternative start site at Met84. Over aa 365-461, human MIB1 shares 99% aa sequence identity with mouse MIB1.
Human/Mouse/Rat Mind Bomb 1/MIB1 Alexa Fluor™ Plus 680‑conjugated Antibody
R&D Systems | Catalog # AF7305AFP680
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Background: Mind Bomb 1/MIB1
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This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
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