Human/Mouse/Rat PBEF/Visfatin Alexa Fluor® 594-conjugated Antibody

Catalog #: FAB40441T Datasheet / COA / SDS

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FAB40441T-100UG

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Human/Mouse/Rat PBEF/Visfatin Alexa Fluor® 594-conjugated Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects human PBEF/Visfatin in direct ELISAs and Western blots.

Source

Monoclonal Mouse IgG1 Clone # 882104

Purification

Protein A or G purified

Immunogen

E. coli-derived recombinant human PBEF/Visfatin
Pro27-His491
Accession # P43790

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 594 (Excitation= 590 nm, Emission= 617 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: PBEF/Visfatin

PBEF (pre-B cell colony-enhancing factor), also known as visfatin and nicotinamide phosphoribosyltransferase, is an approximately 52 kDa member of the NAPRTase family of molecules. It functions both intracellularly and extracellularly, where it participates in NAD synthesis and insulin receptor activation, respectively. Human PBEF is 491 amino acids in length and contains no signal sequence. There is at least one alternative splice form that shows a 5 aa substitution for the C-terminal 128 amino acids (aa 364-491). Over aa 27-491, human PBEF shares 96%, 97%, and 96% aa identity with mouse, porcine, and canine PBEF, respectively.

Long Name

Pre-B-Cell Colony-Enhancing Factor 1

Entrez Gene IDs

10135 (Human); 59027 (Mouse); 297508 (Rat)

Alternate Names

EC 2.4.2.12; MGC117256; NAmPRTase; NAMPT; nicotinamide phosphoribosyltransferase; NMPRTase; PBEF; PBEF1; PBEF1110035O14Rik; PBEF1DKFZp666B131; Pre-B cell-enhancing factor; pre-B-cell colony enhancing factor 1; Pre-B-cell colony-enhancing factor 1; VF; Visfatin