Human/Mouse/Rat PBEF/Visfatin Antibody

Catalog # Availability Size / Price Qty
MAB40441
MAB40441-SP
Detection of Human, Mouse, and Rat PBEF/Visfatin by Western Blot.
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Product Details
Citations (1)
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Human/Mouse/Rat PBEF/Visfatin Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects human PBEF/Visfatin in direct ELISAs and Western blots. 
Source
Monoclonal Mouse IgG1 Clone # 882104
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human PBEF/Visfatin
Pro27-His491
Accession # P43790
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.5-1 µg/mL
See below
Simple Western
5-50 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human, Mouse, and Rat PBEF/Visfatin antibody by Western Blot. View Larger

Detection of Human, Mouse, and Rat PBEF/Visfatin by Western Blot. Western blot shows lysates of 293T human embryonic kidney cell line, RAW 264.7 mouse monocyte/ macrophage cell line, Neuro-2A mouse neuroblastoma cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human/Mouse/Rat PBEF/ Visfatin Monoclonal Antibody (Catalog # MAB40441) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for PBEF/Visfatin at approximately 52 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Western Blot Detection of Human PBEF/Visfatin antibody by Western Blot. View Larger

Detection of Human PBEF/Visfatin by Western Blot. Western blot shows lysates of human heart tissue, 293T human embryonic kidney cell line, and A431 human epithelial carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/ Mouse/Rat PBEF/Visfatin Monoclonal Antibody (Catalog # MAB40441) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for PBEF/Visfatin at approximately 52 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Western Blot Detection of Human PBEF/Visfatin antibody by Western Blot. View Larger

Detection of Human PBEF/Visfatin by Western Blot. Western blot shows lysates of KG-1 human acute myelogenous leukemia cell line and HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/ Mouse/Rat PBEF/Visfatin Monoclonal Antibody (Catalog # MAB40441) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for PBEF/Visfatin at approximately 52 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western Detection of Human PBEF/Visfatin antibody by Simple Western<SUP>TM</SUP>. View Larger

Detection of Human PBEF/Visfatin by Simple WesternTM. Simple Western lane view shows lysates of KG‑1 human acute myelogenous leukemia cell line and HepG2 human hepatocellular carcinoma cell line, loaded at 0.5 mg/mL. A specific band was detected for PBEF/Visfatin at approximately 58 kDa (as indicated) using 50 µg/mL of Mouse Anti-Human/Mouse/Rat PBEF/ Visfatin Monoclonal Antibody (Catalog # MAB40441). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Simple Western Detection of Human and Mouse PBEF/Visfatin antibody by Simple Western<sup>TM</sup>. View Larger

Detection of Human and Mouse PBEF/Visfatin by Simple WesternTM. Simple Western lane view shows lysates of HEK293T human embryonic kidney cell line, RAW 264.7 mouse monocyte/macrophage cell line, and Neuro‑2A mouse neuroblastoma cell line, loaded at 0.2 mg/mL. A specific band was detected for PBEF/Visfatin at approximately 58 kDa (as indicated) using 5 µg/mL of Mouse Anti-Human/Mouse/Rat PBEF/Visfatin Monoclonal Antibody (Catalog # MAB40441). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PBEF/Visfatin

PBEF (pre-B cell colony-enhancing factor), also known as visfatin and nicotinamide phosphoribosyltransferase, is an approximately 52 kDa member of the NAPRTase family of molecules. It functions both intracellularly and extracellularly, where it participates in NAD synthesis and insulin receptor activation, respectively. Human PBEF is 491 amino acids in length and contains no signal sequence. There is at least one alternative splice form that shows a 5 aa substitution for the C-terminal 128 amino acids (aa 364-491). Over aa 27-491, human PBEF shares 96%, 97%, and 96% aa identity with mouse, porcine, and canine PBEF, respectively.

Long Name
Pre-B-Cell Colony-Enhancing Factor 1
Entrez Gene IDs
10135 (Human); 59027 (Mouse); 297508 (Rat)
Alternate Names
EC 2.4.2.12; MGC117256; NAmPRTase; NAMPT; nicotinamide phosphoribosyltransferase; NMPRTase; PBEF; PBEF1; PBEF1110035O14Rik; PBEF1DKFZp666B131; Pre-B cell-enhancing factor; pre-B-cell colony enhancing factor 1; Pre-B-cell colony-enhancing factor 1; VF; Visfatin

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Citation for Human/Mouse/Rat PBEF/Visfatin Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Reduced secretion of neuronal growth regulator 1 contributes to impaired adipose-neuronal crosstalk in obesity
    Authors: E Duregotti, CM Reumiller, U Mayr, M Hasman, LE Schmidt, SA Burnap, K Theofilato, J Barallobre, A Beran, M Grandoch, A Viviano, M Jahangiri, M Mayr
    Nature Communications, 2022-11-25;13(1):7269.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot

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