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Human/Mouse/Rat PKA C Pan Specific Alexa Fluor® 750-conjugated Antibody

Catalog #: AF4175S Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF4175S-100UG

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Rat PKA C Pan Specific Alexa Fluor® 750-conjugated Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects recombinant human PKA C alpha, C beta, and C gamma, and endogenous human PKA C isoforms in Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human PKA Cγ
Met1-Phe351
Accession # P22612
Formulation
Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide
Label
Alexa Fluor 750 (Excitation= 749 nm, Emission= 775 nm)

Applications

Recommended Concentration
Sample
Western Blot
Optimal dilution of this antibody should be experimentally determined.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: PKA C alpha

The cAMP-dependent protein kinase (PKA) holoenzyme is composed of two regulatory and two catalytic subunits, designated PKA R and PKA C, respectively. Three catalytic subunit isoforms, C alpha, C beta, and C gamma, have been identified. Upon PKA R subunit binding to the second messenger cAMP, active PKA C subunits are released, initiating a phosphorylation cascade that regulates such cellular functions as metabolism, ion transport, and gene transcription.

Long Name
cAMP-dependent Protein Kinase Catalytic Subunit alpha
Entrez Gene IDs
5566 (Human); 18747 (Mouse); 25636 (Rat)
Alternate Names
cAMP-dependent protein kinase catalytic subunit alpha; Cs; EC 2.7.11; EC 2.7.11.11; MGC102831; MGC48865; PKA C alpha; PKA C-alpha; Pkaca; PKCA1; PKCD; PRKACA; protein kinase A catalytic subunit; protein kinase, cAMP-dependent, catalytic, alpha

Product Datasheets

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Product Datasheet
COA

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