Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all PKA C (pan) Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human PKA C gamma
Met1-Phe351
Accession # P22612

Specificity

Detects recombinant human PKA C alpha, C beta, and C gamma, and endogenous human PKA C isoforms in Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human/Mouse/Rat PKA C Pan Specific Antibody

Detection of Human PKA C antibody by Western Blot.

Detection of Human PKA C by Western Blot.

Western blot shows lysates of A549 human lung carcinoma cell line, HepG2 human hepatocellular carcinoma cell line, SW480 human colorectal adenocarcinoma cell line, MCF-7 human breast cancer cell line, and MDA-MB-468 human breast cancer cell lines. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat PKA C Pan Specific Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4175) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for PKA C Pan Specific at approximately 42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human/Mouse/Rat PKA C Pan Specific Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: A549 human lung carcinoma cell line, HepG2 human hepatocellular carcinoma cell line, SW480 human colorectal adenocarcinoma cell line, MCF-7 human breast cancer cell line, and MDA-MB-468 human breast cancer cell lines

Reviewed Applications

Read 1 review rated 5 using AF4175 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: PKA C (pan)

The cAMP-dependent protein kinase (PKA) holoenzyme is composed of two regulatory and two catalytic subunits, designated PKA R and PKA C, respectively. Three catalytic subunit isoforms, C alpha, C beta, and C gamma, have been identified. Upon PKA R subunit binding to the second messenger cAMP, active PKA C subunits are released, initiating a phosphorylation cascade that regulates such cellular functions as metabolism, ion transport, and gene transcription.

Long Name

cAMP-dependent Protein Kinase Catalytic Subunit

Alternate Names

CAFD1, PKACA, PPNAD4

UniProt

P22612

Additional PKA C (pan) Products

Product Documents for Human/Mouse/Rat PKA C Pan Specific Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse/Rat PKA C Pan Specific Antibody

For research use only

Customer Reviews for Human/Mouse/Rat PKA C Pan Specific Antibody (1)

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  • Human/Mouse/Rat PKA C Pan Specific Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Endothelial
    Species: Mouse
    Verified Customer | Posted 10/11/2019
    Human/Mouse/Rat PKA C Pan Specific Antibody AF4175

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