Human/Mouse/Rat PTP gamma /PTPRG Antibody

Catalog # Availability Size / Price Qty
MAB4608
MAB4608-SP
Detection of Human and Mouse PTP gamma /PTPRG by Western Blot.
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Product Details
Citations (2)
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Human/Mouse/Rat PTP gamma /PTPRG Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects human, mouse, and rat PTP gamma /PTPRG in Western blots. In Western blots, no cross-reactivity with recombinant human PTPRM, PTPRK, PTPRS, LAR, or DEP1 is observed.
Source
Monoclonal Mouse IgG2B Clone # 481505
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human PTP gamma /PTPRG
Ser111-Ala541
Accession # P23470
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection of Human and Mouse PTP?/PTPRG antibody by Western Blot. View Larger

Detection of Human and Mouse PTP gamma /PTPRG by Western Blot. Western blot shows lysates of CHP-100 human neuroblastoma cell line, SH-SY5Y human neuroblastoma cell line, and Neuro-2A mouse neuroblastoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse/Rat PTP gamma/PTPRG Monoclonal Antibody (Catalog # MAB4608) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for PTP gamma/PTPRG at approximately 160 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PTP gamma/PTPRG

Protein Tyrosine Phosphatase, Receptor type gamma (PTPR gamma ), also called PTPRG and PTPG, is a transmembrane protein. Its extracellular region contains carbonic anhydrase fibronectin III domains, suggesting that it interacts with adhesion molecules, but the only reported activator is an intracellular ATP-dependent factor. During brain development, PTPR gamma is found exclusively in post-mitotic neurons. A wide variety of cancers, including colon, renal clear cell, lung, breast, and cervical cancers, show a high incidence of defects in the PTPR gamma gene, suggesting the protein has a key regulatory function in tumor suppression.

Long Name
Protein Tyrosine Phosphatase Receptor-type Gamma
Entrez Gene IDs
5793 (Human); 19270 (Mouse); 171357 (Rat)
Alternate Names
EC 3.1.3.48; H_RG317H01.1; HPTPG; protein tyrosine phosphatase, receptor type, G; protein tyrosine phosphatase, receptor type, gamma polypeptide; Protein-tyrosine phosphatase gamma; PTP gamma; PTPG; PTPGprotein tyrosine phosphatase gamma; PTPRG; receptor type protein tyrosine phosphatase gamma; receptor tyrosine phosphatase gamma; receptor-type protein phosphatase gamma; receptor-type tyrosine-protein phosphatase gamma; RPTPG; R-PTP-GAMMA

Product Datasheets

Citations for Human/Mouse/Rat PTP gamma /PTPRG Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Protein tyrosine phosphatase receptor type gamma is a JAK phosphatase and negatively regulates leukocyte integrin activation.
    Authors: Mirenda M, Toffali L, Montresor A, Scardoni G, Sorio C, Laudanna C
    J Immunol, 2015;194(5):2168-79.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. Identification of protein tyrosine phosphatase receptor gamma extracellular domain (sPTPRG) as a natural soluble protein in plasma.
    Authors: Moratti E, Vezzalini M, Tomasello L, Giavarina D, Sorio C
    PLoS ONE, 2015;10(3):e0119110.

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Isotype Controls

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Secondary Antibodies

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