Human/Mouse ROR gamma t/RORC2/NR1F3 Alexa Fluor® 647-conjugated Antibody

Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
IC9125R-100UG
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Human/Mouse ROR gamma t/RORC2/NR1F3 Alexa Fluor® 647-conjugated Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects recombinant human ROR gamma t/RORC2/NR1F3 by direct ELISAs and detects human and mouse ROR gamma t/RORC2/NR1F3 in flow cytometry.
Source
Recombinant Monoclonal Rabbit IgG Clone # 1181A
Purification
Protein A or G purified from cell culture supernatant
Immunogen
E. coli-derived recombinant human ROR gamma t/RORC2/NR1F3
Met1-Gln100
Accession # P51449
Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 647 (Excitation= 650 nm, Emission= 668 nm)

Applications

Recommended Concentration
Sample
Intracellular Staining by Flow Cytometry
0.25-1 µg/106 cells
Human peripheral blood mononuclear cells (PBMCs) stimulated to induce Th17 cells and mouse splenoctyes stimulated to induce Th17 cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: ROR gamma t/RORC2/NR1F3

Retinoic acid-related Orphan Receptor gamma (ROR gamma, TOR, RORC; NR1F3) is a member of the orphan nuclear receptor family. ROR gamma is expressed in the muscle, thymus, testis, pancreas, prostate, heart, and liver. ROR gamma plays a role in thymocyte development and homeostasis. RORs bind to DNA as monomers on half-site elements with 5' A/T-rich extensions. An N-terminal isoform of ROR gamma, ROR gamma t, has been shown to be specifically expressed in the thymus.

Long Name
Retinoic Acid-Related Orphan Receptor gamma t
Entrez Gene IDs
6097 (Human)
Alternate Names
IMD42;NR1F3;Nuclear receptor ROR-gamma;RORG;RZRG;RZR-GAMMA;TOR; ROR gamma t; RORC2

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Product Specific Notices


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

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