Human/Mouse ROR gamma t/RORC2/NR1F3 Alexa Fluor® 750-conjugated Antibody

Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
IC9125S-100UG
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Human/Mouse ROR gamma t/RORC2/NR1F3 Alexa Fluor® 750-conjugated Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects recombinant human ROR gamma t/RORC2/NR1F3 by direct ELISAs and detects human and mouse ROR gamma t/RORC2/NR1F3 in flow cytometry.
Source
Recombinant Monoclonal Rabbit IgG Clone # 1181A
Purification
Protein A or G purified from cell culture supernatant
Immunogen
E. coli-derived recombinant human ROR gamma t/RORC2/NR1F3
Met1-Gln100
Accession # P51449
Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 750 (Excitation= 749 nm, Emission= 775 nm)

Applications

Recommended Concentration
Sample
Intracellular Staining by Flow Cytometry
0.25-1 µg/106 cells
Human peripheral blood mononuclear cells (PBMCs) stimulated to induce Th17 cells and mouse splenoctyes stimulated to induce Th17 cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: ROR gamma t/RORC2/NR1F3

Retinoic acid-related Orphan Receptor gamma (ROR gamma, TOR, RORC; NR1F3) is a member of the orphan nuclear receptor family. ROR gamma is expressed in the muscle, thymus, testis, pancreas, prostate, heart, and liver. ROR gamma plays a role in thymocyte development and homeostasis. RORs bind to DNA as monomers on half-site elements with 5' A/T-rich extensions. An N-terminal isoform of ROR gamma, ROR gamma t, has been shown to be specifically expressed in the thymus.

Long Name
Retinoic Acid-Related Orphan Receptor gamma t
Entrez Gene IDs
6097 (Human)
Alternate Names
ROR gamma t; RORC2

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Isotype Controls

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