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Human/Mouse ROR gamma t/RORC2/NR1F3 Alexa Fluor® 750-conjugated Antibody

IC9125S Datasheet / COA / SDS
Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
IC9125S-100UG
Product Details
FAQs
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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse ROR gamma t/RORC2/NR1F3 Alexa Fluor® 750-conjugated Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects recombinant human ROR gamma t/RORC2/NR1F3 by direct ELISAs and detects human and mouse ROR gamma t/RORC2/NR1F3 in flow cytometry.
Source
Recombinant Monoclonal Rabbit IgG Clone # 1181A
Purification
Protein A or G purified from cell culture supernatant
Immunogen
E. coli-derived recombinant human ROR gamma t/RORC2/NR1F3
Met1-Gln100
Accession # P51449
Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 750 (Excitation= 749 nm, Emission= 775 nm)

Applications

Recommended Concentration
Sample
Intracellular Staining by Flow Cytometry
0.25-1 µg/106 cells
Human peripheral blood mononuclear cells (PBMCs) stimulated to induce Th17 cells and mouse splenoctyes stimulated to induce Th17 cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: ROR gamma t/RORC2/NR1F3

Retinoic acid-related Orphan Receptor gamma (ROR gamma, TOR, RORC; NR1F3) is a member of the orphan nuclear receptor family. ROR gamma is expressed in the muscle, thymus, testis, pancreas, prostate, heart, and liver. ROR gamma plays a role in thymocyte development and homeostasis. RORs bind to DNA as monomers on half-site elements with 5' A/T-rich extensions. An N-terminal isoform of ROR gamma, ROR gamma t, has been shown to be specifically expressed in the thymus.

Long Name
Retinoic Acid-Related Orphan Receptor gamma t
Entrez Gene IDs
6097 (Human)
Alternate Names
ROR gamma t; RORC2

Product Datasheets

Product Datasheet
COA
SDS

FAQs

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Isotype Controls

Rabbit IgG Alexa Fluor® 750-conjugated Antibody

IC1051S
Ctrl

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