Human NEDD8 Antibody

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Product Details
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Human NEDD8 Antibody Summary

Antibody does not cross-react with ubiquitn, SUMO, or ISG15 proteins.  Non-functional for immunoprecipitation.
Polyclonal Rabbit IgG
Full-length, highly purified human NEDD8 protein
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Recommended Concentration
Western Blot
Recommended dilution range forWestern blot analysis is 1:100 to 1:1000.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Reconstitution Calculator

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Preparation and Storage

The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

Background: NEDD8

The ubiquitin-like protein NEDD8 is conjugated to targets by the NEDD8-specific E1 activating enzyme (APP-B1/UBA3), the Ubc12 E2 enzyme, and the ROC1/Rbx1 RING FINGER E3 ligase.  NEDD8 plays a critical regulatory role in cell proliferation and development, and modifies nearly all members of the cullin family. 

  1. Gong L. et al. (1999) J. Biol. Chem. 274:12036-12042
  2. Hori T. et al. (1999) Oncogene 18:6829-6834
  3. Kamura T. et al. (1999) Genes. Dev. 13:2928-2933
  4. Kumar S. et al. (1993) Biophys. Biochem. Res. Comm. 195:393-399
  5. Morimoto M. et al. (2003) Biophys. Biochem. Res. Comm. 301:392-398
  6. Wada H. et al. (1999) Biophys. Biochem. Res. Comm. 275:100-105
  7. Whitby F. G. et al. (1998) J. Biol. Chem. 273:34983-34991
Long Name
Neural Precursor Cell Expressed Developmentally Down-Regulated Protein 8
Entrez Gene IDs
4738 (Human); 18002 (Mouse); 25490 (Rat)
Alternate Names
FLJ43224; MGC104393; MGC125896; MGC125897; NEDD8; NEDD-8; Neddylin; Neural precursor cell expressed developmentally down-regulated protein 8; neural precursor cell expressed, developmentally down-regulated 8; Rub1; Ubiquitin-like protein Nedd8

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