Human Neurofascin Alexa Fluor® 750-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB8208S-100UG
R&D Systems Antibodies
1 Image
Product Details
FAQs
Supplemental Products
Reviews

Human Neurofascin Alexa Fluor® 750-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human Neurofascin in direct ELISAs.
Source
Monoclonal Mouse IgG1 Clone # 1018843
Purification
Protein A or G purified from cell culture supernatant
Immunogen
Human embryonic kidney cell, HEK293-derived human Neurofascin
Ile25-Trp1039
Accession # NP_055905
Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 750 (Excitation= 749 nm, Emission= 775 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25-1 µg/106 cells
U87-MG human cell line

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Neurofascin

Neurofascin, a type I transmembrane glycoprotein, is a member of the L1 family of cell adhesion molecules (CAMs) (1-4). L1CAM family members are composed of an extracellular domain (ECD) that contains six immunoglobulin (Ig)-like domains and multiple fibronectin type III repeats, followed by transmembrane and cytoplasmic domains (2, 4, 5). Multiple isoforms of Neurofascin, including NF155, NF166, NF180, and NF186, can be generated by alternative splicing with a predicted range of approximately 70 kDa to 150 kDa (4). These isoforms differ in the combination of fibronectin type III repeats, as well as in the presence of a proline-, alanine-, and threonine-rich segment (PAT domain) located just after the fourth fibronectin type III repeat (4). This recombinant human Neurofascin protein corresponds to rat isoform NF155 and shares 96% amino acid sequence identity with comparable regions of rat and mouse Neurofascin. In rats, NF155 is transiently expressed by oligodendrocytes and Schwann cells during axon myelination (6, 7). NF155 clusters in paranodal regions of oligodendroglia and binds to the Caspr-Contactin complex located on the adjacent axon to form and stabilize paranodal axoglial junctions (8-11). It has been suggested that the ECD of NF155 must be cleaved from oligodendroglia membranes to form and/or stabilize the paranodal structure (12). NF155 has also been shown to promote neuronal adhesion and neurite outgrowth in rats and chickens (12-14). Alterations in NF155 expression have been associated with multiple sclerosis (15).

References
  1. Rathjen, F.G. et al. (1987) Cell 51:841.
  2. Volkmer, H. et al. (1992) J. Cell Biol. 118:149.
  3. Sherman, D.L. and P.J. Brophy (2005) Nat. Rev. Neurosci. 6:683.
  4. Kriebel, M. et al. (2012) Int. J. Biochem. Cell Biol. 44:694.
  5. Liu, H. et al. (2011) J. Biol. Chem. 286:797.
  6. Collinson, J.M. et al. (1998) Glia 23:11.
  7. Basak, S. et al. (2007) Dev. Biol. 311:408.
  8. Charles, P. et al. (2002) Curr. Biol. 12:217.
  9. Schafer, D.P. et al. (2004) J. Neurosci. 24:3176.
  10. Maier, O. et al. (2005) Mol. Cell. Neurosci. 28:390.
  11. Sherman, D.L. et al. (2005) Neuron 48:737.
  12. Maier, O. et al. (2006) Exp. Cell Res. 312:500.
  13. Volkmer, H. et al. (1996) J. Cell Biol. 135:1059.
  14. Koticha, D. et al. (2005) Mol. Cell. Neurosci. 30:137.
  15. Howell, O.W. et al. (2006) Brain 129:3173.
Entrez Gene IDs
23114 (Human); 269116 (Mouse); 116690 (Rat)
Alternate Names
DKFZp686P2250; KIAA0756FLJ46866; neurofascin homolog (chicken); neurofascin homolog; Neurofascin; NF; NFASC; NRCAML

Product Datasheets

You must select a language.

x

Product Specific Notices


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs
Loading...

Reviews for Human Neurofascin Alexa Fluor® 750-conjugated Antibody

There are currently no reviews for this product. Be the first to review Human Neurofascin Alexa Fluor® 750-conjugated Antibody and earn rewards!

Have you used Human Neurofascin Alexa Fluor® 750-conjugated Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review