Human NG2/MCSP Alexa Fluor® 647-conjugated Antibody

    
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  • Species Reactivity
    Human
  • Specificity
    Detects a 220 - 240 kDa cell-surface protein whose N-terminal amino acid sequence is identical to the rat NG2 chondroitin sulfate proteoglycan molecule (1).
  • Source
    Monoclonal Mouse IgG1 Clone # 7.1
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Human transformed stromal cells
  • Formulation
    Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
  • Label
    Alexa Fluor 647
Product Datasheets

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Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    0.25-1 µg/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Example
Detection of NG2/MCSP in G361 Human Cell Line by Flow Cytometry G361 human melanoma cell line was stained with Mouse Anti-Human NG2/MCSP Alexa Fluor® 647‑conjugated Monoclonal Antibody (Catalog # FAB25851R, filled histogram) or isotype control antibody (Catalog # IC002R, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: NG2/MCSP
The clone 7.1 antibody recognizes a cell surface-expressed chondroitin sulfate proteoglycan that is the human homolog of rat NG2 antigen. The NG2 antigen, also known as chrondroitin sulfate proteoglycan 4, can be found on human glial cell population and is also expressed on myeloid and lymphoid cell subsets with chromosomal modifications.
  • References:
    1. Smith, F.O. et al. (1996) Blood. 87:1123.
  • Long Name:
    Chondroitin Sulfate Proteoglycan NG2/Melanoma Associated Chondroitin Sulfate Proteoglycan
  • Entrez Gene IDs:
    1464 (Human); 121021 (Mouse); 81651 (Rat)
  • Alternate Names:
    AN2; chondroitin sulfate proteoglycan 4 (melanoma-associated); chondroitin sulfate proteoglycan 4; CSPG4; EC 3.6.3; HMW-MAA; MCSP; MCSPChondroitin sulfate proteoglycan NG2; MCSPG; MCSPGMelanoma chondroitin sulfate proteoglycan; MEL-CSPG; MSK16Melanoma-associated chondroitin sulfate proteoglycan; NG2; NG2EC 2.7.8
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Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG1 Alexa Fluor® 647-conjugated Antibody

Ctrl IC002R
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Staining Buffer (1X)

Flow FC001 10
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 5
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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