Human NRAGE Alexa Fluor® 594-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB3835T-100UG
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Human NRAGE Alexa Fluor® 594-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human NRAGE in direct ELISAs.
Source
Monoclonal Mouse IgG2b Clone # 353124
Immunogen
E. coli-derived recombinant human NRAGE
Met1-Glu778
Accession # Q9Y5V3
Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 594

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25-1 µg/106 cells
HEK293 human embryonic kidney cell line

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: NRAGE

Neurotrophin receptor-interacting MAGE homolog (NRAGE, also known as MAGE-D1 or Dlxin-1) is a ubiquitously expressed cytosolic protein that is a member of the melanoma-associated antigen (MAGE) family. The 778 aa human NRAGE protein contains a segment with 22 repeats of a W(P/Q)xPxx motif, followed by a MAGE domain. In the brain, NRAGE interacts with p75NTR, blocks cell cycle progression and facilitates NGF-dependent apoptosis. Over amino acids 73‑254, human NRAGE shares 87% and 73% aa identity with mouse and rat NRAGE, respectively.

Long Name
Neurotrophin Receptor-interacting MAGE Homolog
Entrez Gene IDs
9500 (Human); 94275 (Mouse); 84469 (Rat)
Alternate Names
Dlxin-1; MAGE tumor antigen CCF; MAGED1; melanoma antigen family D, 1; melanoma-associated antigen D1; Neurotrophin receptor-interacting MAGE homolog; NRAGE MAGE-D1 antigen; NRAGE

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