Human PAG1 Antibody

R&D Systems | Catalog # MAB5285

R&D Systems
Discontinued Product
MAB5285 has been discontinued. View all PAG1 products.

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Mouse

Applications

Validated:

Western Blot, Simple Western

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 504004
View all PAG1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived human PAG1
Ser38-Leu432
Accession # Q9NWQ8

Specificity

Detects human PAG1 in direct ELISAs and Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human PAG1 Antibody

Detection of Human PAG1 antibody by Western Blot.

Detection of Human PAG1 by Western Blot.

Western blot shows lysates of Daudi human Burkitt's lymphoma cell line and Raji human Burkitt's lymphoma cell line. PVDF Membrane was probed with 1 µg/mL of Human PAG1 Polyclonal Antibody (Catalog # MAB5285) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Specific bands were detected for PAG1 at approximately 70-85 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human PAG1 antibody by Simple WesternTM.

Detection of Human PAG1 by Simple WesternTM.

Simple Western lane view shows lysates of Raji human Burkitt's lymphoma cell line and Daudi human Burkitt's lymphoma cell line, loaded at 0.2 mg/mL. A specific band was detected for PAG1 at approximately 76 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human PAG1 Monoclonal Antibody (Catalog # MAB5285). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Applications for Human PAG1 Antibody

Application
Recommended Usage

Simple Western

10 µg/mL
Sample: Raji human Burkitt's lymphoma cell line and Daudi human Burkitt's lymphoma cell line

Western Blot

1 µg/mL
Sample: Daudi human Burkitt's lymphoma cell line and Raji human Burkitt's lymphoma cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: PAG1

PAG1 (Phosphoprotein-associated with glycosphingolipid enriched microdomains 1; also Cbp/Csk-binding protein) is a 47 kDa protein that runs anomalously at 70‑85 kDa in SDS PAGE. PAG1 is a constitutively phosphorylated and palmitoylated protein that occurs in leukocyte lipid rafts. It negatively regulates Src family kinases by linking Csk to FynT. Human PAG1 is 432 amino acids (aa) in length. It is a type III (no signal sequence) transmembrane protein that contains a 16 aa extracellular region (aa 1-16) and a 395 aa cytoplasmic domain (aa 38-432). PAG1 is palmitoylated at Cys37 and phosphorylated at Tyr317 where it interacts with Csk. There is an FynT binding site at aa 131-137. One splice variant shows a 36 aa substitution for aa 293-432.

Long Name

Phosphoprotein Associated with Glycosphingolipid Microdomains 1

Alternate Names

CBP

Entrez Gene IDs

55824 (Human); 94212 (Mouse)

Gene Symbol

PAG1

UniProt

Q9NWQ8 (Human)

Additional PAG1 Products

Product Documents for Human PAG1 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human PAG1 Antibody

For research use only

Related Research Areas

Mast Cells

Citations for Human PAG1 Antibody

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