Human PAR1 Alexa Fluor® 488-conjugated Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human PAR1 in direct ELISAs and Western blots.
  • Source
    Monoclonal Mouse IgG2B Clone # 731115
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant human PAR1
    Arg27-Thr102, Ser375-Thr425
    Accession # P25116
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Alexa Fluor 488
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    5 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of PAR1 in Human Peripheral Blood Platelets by Flow Cytometry. Human peripheral blood platelets were stained with Mouse Anti-Human Integrin  alpha 2b/CD41 APC‑conjugated Monoclonal Antibody (Catalog # FAB7616A) and either (A) Mouse Anti-Human PAR1 Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # FAB3855G) or (B) Mouse IgG2B Alexa Fluor 488 Isotype Control (Catalog # IC0041G). View our protocol for Staining Membrane-associated Proteins.
Detection of PAR1 in HT‑29 Human Cell Line by Flow Cytometry. HT‑29 human colon adenocarcinoma cell line was stained with Mouse Anti-Human PAR1 Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # FAB3855G, filled histogram) or isotype control antibody (Catalog # IC0041G, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: PAR1

Human Proteinase-Activated Receptor 1 (hPAR1), also known as thrombin receptor, is a 65-70 kDa, 399 amino acid long member of the seven-transmembrane superfamily of cell-surface G protein-coupled receptors. PAR1 is activated by thrombin cleavage of its N-terminal propeptide in the extracellular domain. Human PAR1 is widely expressed in many cell types including endothelial cells, and it has been implicated in a variety of inflammatory responses. Over the regions used as immunogen, human and mouse PAR1 proteins are 58% identical in the region spanning the propeptide and extracellular domains, and 84% identical in the cytoplasmic tail.

  • Long Name:
    Protease-Activated Receptor 1
  • Entrez Gene IDs:
    2149 (Human); 14062 (Mouse); 25439 (Rat)
  • Alternate Names:
    Cf2r; CF2RHTR; coagulation factor II (thrombin) receptor; Coagulation factor II receptor; F2R; HTR; PAR1; PAR1PAR-1protease-activated receptor 1; Protease-Activated Receptor 1; Thrombin receptor; ThrR; TRGPC; TRproteinase-activated receptor 1
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG2B Alexa Fluor® 488-conjugated Isotype Control

Ctrl IC0041G 2  
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Staining Buffer (1X)

Flow FC001 6
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 4
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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