Human PAR1 Alexa Fluor® 488-conjugated Antibody

Catalog # Availability Size / Price Qty
Detection of PAR1 in HT‑29 Human Cell Line by Flow Cytometry.
2 Images
Product Details
Citations (2)
Supplemental Products

Human PAR1 Alexa Fluor® 488-conjugated Antibody Summary

Species Reactivity
Detects human PAR1 in direct ELISAs and Western blots.
Monoclonal Mouse IgG2B Clone # 731115
Protein A or G purified from hybridoma culture supernatant
E. coli-derived recombinant human PAR1
Arg27-Thr102, Ser375-Thr425
Accession # P25116
Supplied in a saline solution containing BSA and Sodium Azide.
Alexa Fluor 488 (Excitation= 488 nm, Emission= 515-545 nm)


Recommended Concentration
Flow Cytometry
5 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of PAR1 antibody in HT‑29 Human Cell Line antibody by Flow Cytometry. View Larger

Detection of PAR1 in HT‑29 Human Cell Line by Flow Cytometry. HT-29 human colon adenocarcinoma cell line was stained with Mouse Anti-Human PAR1 Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # FAB3855G, filled histogram) or isotype control antibody (Catalog # IC0041G, open histogram). View our protocol for Staining Membrane-associated Proteins.

Flow Cytometry Detection of PAR1 antibody in Human Peripheral Blood Platelets antibody by Flow Cytometry. View Larger

Detection of PAR1 in Human Peripheral Blood Platelets by Flow Cytometry. Human peripheral blood platelets were stained with Mouse Anti-Human Integrin a2b/CD41 APC-conjugated Monoclonal Antibody (Catalog # FAB7616A) and either (A) Mouse Anti-Human PAR1 Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # FAB3855G) or (B) Mouse IgG2BAlexa Fluor 488 Isotype Control (Catalog # IC0041G). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: PAR1

Human Proteinase-Activated Receptor 1 (hPAR1), also known as thrombin receptor, is a 65-70 kDa, 399 amino acid long member of the seven-transmembrane superfamily of cell-surface G protein-coupled receptors. PAR1 is activated by thrombin cleavage of its N-terminal propeptide in the extracellular domain. Human PAR1 is widely expressed in many cell types including endothelial cells, and it has been implicated in a variety of inflammatory responses. Over the regions used as immunogen, human and mouse PAR1 proteins are 58% identical in the region spanning the propeptide and extracellular domains, and 84% identical in the cytoplasmic tail.

Long Name
Protease-Activated Receptor 1
Entrez Gene IDs
2149 (Human); 14062 (Mouse); 25439 (Rat)
Alternate Names
Cf2r; CF2RHTR; coagulation factor II (thrombin) receptor; Coagulation factor II receptor; F2R; HTR; PAR1; PAR1PAR-1protease-activated receptor 1; Protease-Activated Receptor 1; Thrombin receptor; ThrR; TRGPC; TRproteinase-activated receptor 1

Product Datasheets

You must select a language.


Product Specific Notices

This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

Citations for Human PAR1 Alexa Fluor® 488-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
Filter your results:

Filter by:

  1. Interplay of Plasmodium falciparum and thrombin in brain endothelial barrier disruption
    Authors: M Avril, M Benjamin, MM Dols, JD Smith
    Sci Rep, 2019;9(1):13142.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Thrombin-Induced Calpain Activation Promotes Protease-Activated Receptor 1 Internalization.
    Authors: Alejandro Alvarez-Ar, Irene Lee-River, Edith López, Arturo Hernández, Ana María López-Col
    International Journal of Cell Biology, 2017;0(0):1687-8876.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry


No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Reviews for Human PAR1 Alexa Fluor® 488-conjugated Antibody

There are currently no reviews for this product. Be the first to review Human PAR1 Alexa Fluor® 488-conjugated Antibody and earn rewards!

Have you used Human PAR1 Alexa Fluor® 488-conjugated Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review